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The mechanism of PPP2R1B genein colorectal cancers

Research Project

Project/Area Number 13671291
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionFukui Medical University

Principal Investigator

GOI Takanori  Fukui Medical University, Faculty of Medicine, Assistant, 医学部, 助手 (60225638)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Akio  Fukui Medical University, Faculty of Medicine, Professor, 医学部, 教授 (10174608)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsPPP2R1B gene / Protein phosphatase / PP2A protein / colorectal cancer / PPP2R1B / PP2A-A蛋白質 / プロテインフォスファターゼ
Research Abstract

Background : The chromosome region 11q is frequently deleted in colorectal cancers. The PPP2R1B tumor suppressor gene, encoding the β isoform of the A subunit of serine/threonine-specific protein phosphatase 2A (PP2A-Aβ), located at 11q22-23, is inactivated in patients with cancer. The present study determines whether or not PP2A-Aβ is altered in colorectal cancers.
Method : We searched for alterations of the PPP2R1B gene and interactions between PP2A-Aβ and PP2A-C proteins in 50 surgically resected colorectal cancer tissues.
Results : Missense mutations and homozygous deletions of the PPP2R1B gene were found in four of 50 patients (8 %) and in one of 50 patients, respectively, with colorectal cancers. Deletions and/or point mutations within 412-601 amino acid sequences (binding regions of PP2A-C protein) of the PPP2R1B gene derived from colorectal cancer tissues inhibited co-immunoprecipitation of PP2A-Aβ and PP2A-C proteins.
Conclusion : These finding suggest that the PPP2R1B gene functions as a tumor suppressor gene and acts as a molecular switch that becomes active in response to specific upstream signals. Upon activation, the gene alters the activities of specific downstream target proteins for the cell cycle regulations and/or metabolism in some colorectal cancers.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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