• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The protective effect of ischemic preconditioning on small intestinal ischemia-reperfusion injury

Research Project

Project/Area Number 13671315
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKagoshima University

Principal Investigator

HAMADA Nobuo  Kagoshima University, University Hospital, Faculty of Medicine, assistant professor, 医学部, 講師 (30253868)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Noboru  Kagoshima University, University Hospital, Faculty of Medicine, Research Associate, 医学部附属病院, 助手
KOMOKATA Teruo  Kagoshima University, University Hospital, Faculty of Medicine, Research Associate, 医学部附属病院, 助手
石崎 直樹  鹿児島大学, 医学部・附属病院, 助手
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsSmall intestine / Ischemia-reperfusion injury / Ischemic preconditioning / K_<ATP> channel / Ischemic Preconditioning / ATP感受性Kチャンネル
Research Abstract

While ischemic preconditioning (IP) has been shown to have protective effect for ischemia-reperfusion injury (IRI) in several organ systems, little is known about the effect on intestinal IRI. In this study, we investigated the immediate protective effect of IP on intestinal IRI.
The entire canine small intestine was isolated on a vascular pedicle that consisted of the proximal superior mesenteric artery and vein. Complete intestinal warm ischemia was induced by clamping these vessels. The dogs were randomized into four groups (each n=5) : Group I -control group, animals subjected to 120 min of intestinal ischemia, followed by 240 min of reperfusion. Group II -ischemic preconditioning group (IP) : Before the ischemia reperfusion period (as in group I), animals were subjected to previous ischemic preconditioning with 15 min of ischemia and 15 min of reperfusion. Group III -nicorandil (Nic) treated group, same as group I, but nicorandil, a selective K_<ATP> channel opener, was given to animals with bolus injection of 100 μg/kg/min during the reperfusion. Group IV -glibenclamide (Gli) treated group, same as group II, but 0.3 mg/kg of glibenclamide, selective K_<ATP> channel blocker, was given intravenously 15 min before IPC.
Compared to the control, the preconditioned group reduced CPK and lactate levels in the portal vein, maintained the intestinal mucosal pH (pHi) and attenuated the histological tissue damage after reperfusion. Nic treatment showed better outcome of pHi, and Gli administration attenuated the ischemic preconditioning effect.
These results suggest that IP can protect the intestine from the prolonged ischemia and reperfusion injury and that the K_<ATP> channel plays an important role in this protection mechanism.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 海江田 衛, 濱田信男, 石崎直樹, 福枝幹雄, 坂田隆造, 吉田浩巳: "小腸に対するIschemic Preconditioningの作用"ICUとCCU. 26. 1035-1039 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 海江田 衛, 濱田信男, 石崎直樹, 福枝幹雄, 坂田隆造, 吉田浩巳: "小腸虚血再灌流障害に対するIschemic Preconditioning効果およびその機序の実験的検討"日本消化器外科学会雑誌(学会抄録). 34・7. 365 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kaieda M, Hamada N, Ishizaki N, Hukueda M, Sakata R, Yoshida H: "The protective effect of ischemic preconditioning on small intestinal ischemia-reperfusion injury"ICU&CCU. 26. 1035-1039 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kaieda M, Hamada N, Ishizaki N, Hukueda M, Sakata R, Yoshida H: "Mechanism of the protective effect of ischemic preconditioning on small intestinal ischemia-reperfusion injury"Jpn J Gastroenterol Surg. 34(7). 365 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 海江田 衛: "小腸に対するIschemic Preconditioningの作用"ICUとCCU. 26・12. 1035-1039 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi