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Analysis by GeneFilters of the effect of radiation on esophageal squamous cell carcinomas

Research Project

Project/Area Number 13671352
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionNippon Medical School

Principal Investigator

BO Hideki  Nippon Medical School, Center for Digestive Diseases, Assistant Professor, 医学部, 助手 (20333122)

Co-Investigator(Kenkyū-buntansha) KAWANAMI Oichi  Nippon Medical School Institute of Gerontology, Department of Molecular Pathology, Professor, 老人病研究所, 教授 (70096973)
GHAZIZADEH Mohammad  Nippon Medical School Institute of Gerontology, Department of Molecular Pathology, Associate Professor, 老人病研究所, 助教授 (30190979)
KURIHARA Yuji  Nippon Medical School, Center for Digestive Diseases, Assistant Professor, 医学部, 助手 (80267161)
MORIYAMA Yukichi  Nippon Medical School, Center for Digestive Diseases, Professor, 医学部, 教授 (40089701)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsEsophageal squamous cell carcinoma / Radiation / cDNA microarray / gene expression
Research Abstract

The mechanism of radiation therapy for esophageal carcinoma is not well understood. To examine the effect of radiation therapy on squamous cell carcinoma at molecular levels, we performed GeneFilters analyses as follows: Three different cell lines comprising of well, moderately, and poorly differentiated squamous cell carcinomas were used. Each cell line was subjected to 2, 4, and 8 Gy radiation. RNA was extracted from each cell line at 0, 15, and 30 min after radiation. Each RNA sample was labeled with 33P and hybridized to GeneFilters (Named Genes; GF-211), filter image was scanned and analyzed using Pathways 3.0 software. Several genes related to cell cycling were down-regulated and several others related to apotosis and matrix synthesis were up-regulated after treatment of the cells with radiation. We also validated some of these genes by a semiquantitative RT-PCR. Overall, we found that radiation at 8 Gy dose gave the best effect at 30 min after radiation. We plan to study the function of these genes in relation to radiation effect in these cell lines.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Fujiwara M, Jin E, Ghazizadeh M, Kawanami O.: "An in vitro model to evaluate regulatory mechanisms of antigen expression by normal pulmonary vessel endothelial cells"Microvasc.Res.. 61・2. 215-219 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai J, Ghazizadeh M, Naito Z, Asano G.: "Immunohistochemical expression of T, Tn and sialyl-Tn antigens and clinical outcome in human breast carcinoma"Anticancer Res.. 21・2B. 1327-1334 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ozaki N, Ishizaki M, Ghazizadeh M, Yamanaka N.: "Apoptosis mediates decrease in cellularity during the regression of Arthus reaction in cornea"Br.J.Ophthalmol.. 85・5. 613-618 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshimura C, Miyamasu M, Nagase H, Iikura M, Kawanami O, et al.: "Morita Y, Iwata T, Yamamoto K, Hirai K. : Glucocorticoids induce basophil apoptosis"J.Allergy Clin.Immunol.. 108・2. 215-220 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jin E, Ghazizadeh M, Fujiwara M, Nagashima M, Kawanami O, et al.: "Angiogenesis and phenotypic alteration of alveolar capillary endothelium in areas of neoplastic cell spread in primary lung adenocarcinoma"Pathol.Int.. 51・9. 691-700 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi T, Azuma A, Abe S, Kawanami O, Ohara K, Kudoh: "Significance of lymphocytosis in bronchoalveolar lavege in suspected ocular sarcoidosis"Eur.Respir.J.. 18・3. 515-521 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jin E, Fujiwara M, Nagashima M, Shimizu H, Kawanami O, et al.: "Aerogenous spread of primary lung adenocarcinoma induces ultrastructural remodeling of the alveolar capillary endothelium"Hum.Pathol.. 32・10. 1050-1058 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamano G, Funahashi H, Kawanami O, Zhao LX, Ban N, Uchida Y, et al.: "ABCA3 is a lamellar body membrane protein in human lung alveolar type II cells(1)"FEBS Lett. 16・508. 221-225 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ghazizadeh M, Kawanami O, et al.: "Assessment of gene expression profile by cDNA microarray analysis"Journal of Nippon Medical School. 68・6. 460-461 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujiwara M, Jine E, Ghazizadeh M, Kawanami O: "Antisense oligodeoxynucleotides against thrombomodulin suppress the cell growth of lung adenocarcinoma cell line A549 cells"Pathol.Int.52 : 204-213. 2002 Apr.. 52・20. 204-213 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Obara K, Ghazizadeh M, Shimizu H, Arai R, Tenjin T, Kawanami O, et al.: "Comprative genomic hybridization study of genetic changes associated with vindesine resistance in esophageal carcinoma"Int.J.Oncology. 20(2) : 255-260. 2002 Feb.. 20・2. 255-260 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurihara Y, Ghazizadeh M, Bo H, Shimizu H, Kawanami O, et al.: "Genome-wide screening of laser capture microdissected gastric signet-ring cell carcinomas"J Nippon Medical School. 69・3. 235-242 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shichinohe K, Shimizu SM, Ghazizadeh M, Ishizaki M.: "Supplementation of heterologous complement induces anti-Thy-1.1 nephritis in the mongolian gerbil (Merones unguiculatus)"Laboratory Animal Science. 64. 463-467 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimizu H, Fukuda T, Ghazizadef M, et al.: "Molecular cytogenetic characterization of drug-resistant leukemia cell lines by comparative genomic hybridization and fluorescence in situ hybridization"Jpn.J.Cancer Res.. 96. 902-910 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jin E, Fujiwara M, Ghazizadeh M, Takemura T, kawanami O: "Protease-activated receptor (PAR)-1 and PAR-2 participate in the cell growth of alveolar capillary endothelium in primary lung adenocarcinomas"Cancer. 97. 703-713 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsushima M, Ghazizadeh M, Konishi H, Araki T: "Association of ovarian tumor epithelium coexpressing HLA-DR and CA-125 Antigens with tumor infiltrating cytotoxic T lymphocytes"J.Nippon Med.Sch.. 70・1. 40-44 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Niyazi M, Ghazizadeh M, Konishi H, Kawanami O, et al.: "Expression of p73 and c-Abl proteins In human ovarian carcinomas"J.Nippon Med.Sch.. 70(In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujiwara M, Jin E, Ghazizadeh M, Kawanami O: "An in vitro model to evaluate regulatory mechanisms of antigen expression by normal pulmonary vessel endothelial cells."Micro vase. Res.. 61. 215-219 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai J, Ghazizadeh M, Naito Z, Asano G: "Immunohistochemical expression of T, Tn and sialyl-Tn antigens and clinical outcome in human breast carcinoma."Anti cancer Res.. 21. 1327-1334 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ozaki N, Ishizaki M, Ghazizadeh M, Yamanaka N: "Apoptosis mediates decrease in cellularity during the regressioniof Arthus reaction in cornea."Br. J. Ophthalmol.. 85. 613-618 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshimura C, Miyamasu M, Nagase H, Iikura M, Yamaguchi M, Kawanami O, Morita Y, Iwata T, Yamamoto K, Hirai K: "Glucocorticoids induce basophil apoptosis."J. Allerg. Clin. Immunol.. 108. 215-220 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi T, Azuma A, Abe S, Kawanami O, Ohara K, Kudoh: "Significance of lymphocytosis in bronchoalveolar lavage in suspected ocular sarcoidosis."Eur. Respir. J.. 18. 515-521 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jin E, Ghazizadeh M, Fujiwara M, Nagashima M, Shimizu H, Ohaki Y, Arai S, Gomibuchi M, Takemura T, Kawanami O: "Angiogenesis and phenotypic alteration of alveolar capillary endothelium in areas of neoplastic cell spread in primary lung adenocarcinoma."Pathol. Int.. 51. 691-700 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jin E, Fujiwara M, Nagashima M, Shimizu H, Ghazizadeh M, Pan X, Arai S, Ohaki Y, Gomibuchi M, Takemura T, Kawanami O: "Aerogenous spread of primary lung adenocarcinoma induces ultrastructural remodeling of the alveolar capillary endothelium."Hum. Pathol.. 32. 1050-1058 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamano G, Funahashi H, Kawanami O, Zhao LX, Ban N, Uchida Y, Morohoshi T, Ogawa J, Shioda S, Inagaki N: "ABCA3 is a lamellar body membrane protein in human lung alveolar type II cells(1)"FEBS Lett.. 16:508. 221-225 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ghazizadeh M, Kawanami O, Araki T: "Assessment of gene expression profile by cDNA microarray analysis."J. Nippon Med. Sch.. 68. 460-461 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Obara K, Ghazizadeh M, Shimizu H, Arai R, Tenjin T, Suzuki S, Moriyama Y, Kawanami O: "Comprative genomic hybridization study of genetic changes associated with vindesine resistance in esophageal carcinoma."Int. J. Oncol.. 20. 255-260 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujiwara M, Jin E, Ghazizadeh M, Kawanami O: "Antisense oligodeoxynucleotides against thrombomodulin suppress the cell growth of lung adenocarcinoma cell line A549 cells."Pathol. Int.. 52. 204-213 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurihara Y, Ghazizadeh M, Bo H, Shimizu H, Kawanami O, Moriyama Y, Onda M: "Genome-wide screening of laser capture microdissected gastric signet-ring cell carcinomas."J. Nippon Med. Sch.. 63. 235-242 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shichinohe K, Shimizu SM, Ghazizadeh M, Ishizaki M: "Supplementation of heterologous complement induces anti-Thy-1.1 nephritis in the mongolian gerbil (Merones unguiculatus)."Lab. Animal Sci.. 64. 463-467 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimizu H, Fukuda T, Ghazizadeh M: "Molecular cytogenetic characterization of drug-resistant leukemia cell lines by comparative genomic hybridization and fluorescence in situ hybridization."Jpn. J. Cancer Res.. 96. 902-910 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Feng H, Ghazizadeh M, Konishi H, Araki T: "Expression of MUC1 and MUC2 mucin gene products in human ovarian carcinomas."Jpn. J. Clin. Oncol.. 32. 525-529 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jin E, Fujiwara M, Ghazizadeh M, Takemura T, Kawanami O: "Protease-activated receptor (PAR)-1 and PAR-2 participate in the cell growth of alveolar capillary endothelium in primary lung adenocarcinomas"Cancer. 97. 703-713 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsushita M, Ghazizadeh M, Konishi H, Araki T: "Association of ovarian tumor epithelium coexpressing HLA-DR and CA-125 antigens with tumor infiltrating cytotoxic T lymphocytes."J. Nippon Med. Sch.. 70. 40-44 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Niyazi M, Ghazizadeh M, Konishi H, Kawanami O, Sugisaki Y, Araki T: "Expression of p73 and c-Abl proteins In human ovarian carcinomas."J. Nippon Med. Sch.. 70(in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ghazizadeh Mohammad: "Assessment of gene expression profile by cDNA microarray analysis"Journal of Nippon Medical School. 68・6. 460-461 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kurihara Y, Ghazizadeh M, et al.: "Genome-wide screening of laser capture microdissected gastric signet-ring cell carcinomas"J Nippon Medical School. 69・3. 235-242 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimizu H, Fukuda T, Ghazizadeh M, et al.: "Molecular cytogenetic characterization of drug-resistant leukemia cell lines by comparative genomic hybridization and fluorescence in situ hybridization"Jpn. J. Cancer Res.. 96. 902-910 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuji Kurihara: "Genome-Wide Screening of Laser Capture Microdissected Gastric Signet-Ring Cell Carcinomas"Journal of Nippon Medical School. Vol.69 No3. (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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