| Project/Area Number |
13671353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
YOSHIDA Keisuke The Nippon Dental University School of Dentistry at Niigata, Department of Surgery, Professor, 新潟歯学部, 教授 (90018680)
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| Co-Investigator(Kenkyū-buntansha) |
KUROSAKI Isao Niigata University, Department of Surgery of Niigata University Hospital, Associate Professor, 医学部附属病院, 講師 (50293220)
SHIRAI Yoshio Div. of Digestive and General Surgery, Department of Regeneration and Transplant Medicine Course for Biological Functions and Medical Control, Niigata University Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 講師 (50216173)
OHTAKE Masahiro The Nippon Dental University School of Dentistry at Niigata, Department of Surgery, Associate Professor, 新潟歯学部, 講師 (30328869)
IWAFUCHI Mitsuya Department of Medical Technology, School of Health Sciences Faculty of Medicine, Niigata University, Professor, 医学部, 教授 (70143766)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Gallbladder cancer / Hericobacter / Hericobacter infection of the biliary tract |
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| Research Abstract |
To clarify the role of helicobacter infection in the pathogenesis of biliary tract cancer, evidence of helicobacter infection in patients who had cholecys^ectomy for the treatment of biliary tract diseases was investigated.
Sixty-six patients who had biliary surgery between June 2001 and August 2002 were studied.
Using removed gallbladder tissue and bile, Helicobacter specific genomic DNA was examined by Polymerase Chain Reaction (PCR) for 16S rRNA and by nested PCR for 860 bp genomic DNA fragment. 16S rRNA was positive in only one gallbladder tissue and helicobacter pylori specific 860 bp DNA fragment was detected hi 12 patients (18.2%). However, culture of gallbladder tissue and bile for helicobacter was negative in all. Histological examination revealed Helicobacter-4ikerod^brm microorganism in only one gallbladder specimen.
Conclusion: Even though DNA fragment of helicobacer (probably of H.pylori) was detected in 18 % of the patients, our results did not support the presumption that we have significantly high prevalence of biliary tract helicobacter infection among patients of Niigata district where was well known as the high risk area for biliary tract cancer. However, a recent report from Tokyo described the surprisingly high incidence of helicobacter bilis specific 16S rRNA in bile of Japanese and Thai patients with biliary diseases. It was considered important to reconstruct research program focusing on H.bilis using H.bilis specific culture method and H.bilis specific PCR system.
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