Studies on the invasive potential of malignant gliomas
| Project/Area Number |
13671469
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Teikyo University |
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Principal Investigator |
NAGASHIMA Tadashi Teikyo University, Shool of Medicine, Professor (70217991)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUNO Akira Teikyo University, School of Medicine, Associate Professor (00242058)
TANAKA Hideki Teikyo University, School of Medicine, Staff Assistant (50276713)
KATAYAMA Haruko Teikyo University, School of Medicine, Staff Assistant (40307187)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | invasion / fast blue / HGF / MIB-1SI / P53 / GEAP / fibronectin / グリオーマ / 多発性腫瘍 / 弧発性腫瘍 / MIB-1 / ファイブロネクチン / ラミニン / ヌードラット / 蛍光染色 / トグリオーマ株細胞 / 腫瘍細胞浸潤 / U251MG / U87MG |
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| Research Abstract |
5×10^5U87MG cells stained with fast blue were inoculated in to the right basal ganglia of nude rat (F344/Njcl-mu). Five and 10 days after inoculation, hepatocytic growth factor (HGF) was injected into the brain of each nude rat. Two to three weeks after tumor inplantation, the brain of each nude rat were excised, fixed, and cut into 6 micro mm frozen sections.We observed the brain tumor and its surrounding brain tssue of each frozen section with fluorescein microscope. We found that tumor cells were invaded into the sorrounding brain tissue markedly as compared with control section which prepared from the nude rat without HGF administration. This result suggests that HGF increase the invasive potential of glial tumor cells.
We investigated the biological characteristics such as proliferative potential estimated from MIB-1 SI, and expression of P53 GFAP, fibronectin of multicentric glioblastomas, and compare them with those of singlecentric glioblastomas. In this study, paraffin enbedded sections of four multicentric glioblastomas and five singlecentric glioblastomas were used for immunohistochemical staining of MIB-1, P53, GEAP and fibronectin. The value of MIB-1SI and the degree of expression of P53, GFAP, fibronectin appere to be no significant difference between multicentric glioblastomas and singlecentric glioblastomas. These results suggest that some other intrinsic factors, such as MMP-2 and VEGF, may participate in invasion of glioblastomas.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] 腫瘍マーカー2001
Author(s)
長島正
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Journal Title
Clinical : Neuroscience 19
Pages: 307-309
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Confocal Laser Scaning Microscopy2001
Author(s)
Matsuno A, Itoh J, Nagashima T, Osamura Y, Watanabe K
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Journal Title
Morphology Metuods Cell and Molecular Biology Techniques (Lloyd RV ed) (Human Press) 10
Pages: 165-180
Description
「研究成果報告書概要(欧文)」より
Related Report
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