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THE EFFECT OF INTRINSIC FIBROBLASTS ON REMODELING OF PATELLAR TENDON MATRIX IN RESPONSE TO OVER- STRESSING

Research Project

Project/Area Number 13671480
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

AOKI Yoshimitsu  Hokkaido Univ., Medical Hospital, Lect., 医学部附属病院, 講師 (10192858)

Co-Investigator(Kenkyū-buntansha) TOHYAMA Harukazu  Hokkaido Univ., Grad. School of Medicine, Asso. Prof., 大学院・医学研究科, 助教授 (60301884)
YASUDA Kazunori  Hokkaido Univ., Grad. School of Medicine, Prof., 大学院・医学研究科, 教授 (20166507)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsTendon / Ligament / Stress enhancement / Ligament reconstruction / Collagen / Remodeling / Fibroblasts / Extracellular matrix / 膝蓋腱 / 自家腱移植 / 力学的負荷 / リモデリング / 細胞外マトリクス / 靭帯 / 力学的環境 / スポーツ
Research Abstract

Methods :
1) Japanese White rabbits were divided into four groups.
2) In Group 1, the patellar tendon underwent the in situ freeze-thaw treatment to kill intrinsic fibroblasts of the patellar tendon and the patellar tendon underwent the wrapping treatment with nylon membrane filters to inhibit extrinsic cell infiltration.
3) In Group 2, the medial and lateral portions of the frozen-thawed patellar tendon were resected to enhance the stress, and then the central two-thirds of the patellar tendon underwent the wrapping treatment.
4) In Group 3, the patellar tendon without the freeze/thaw treatment underwent the wrapping treatment.
5) In Group 4, the patellar tendon was narrowed and wrapped in the same manner.
6) All rabbits were killed at 6 weeks after surgery. We examined the in situ frozen-thawed patellar tendon at 6 weeks after the treatment, biomechanically and histologically.
Results :
1) The elastic modulus and the tensile strength of the patellar tendon in Group 2 were significantly less than those in Group 1, while we could not find any significant differences in these parameters between Groups 3 and 4.
2) Histologically, no fibroblasts were observed in Groups 1 and 2, while fibroblasts were found in Groups 3 and 4.
Conclusion:
This study revealed that stress enhancement decreases the elastic modulus and the tensile strength of the extracellular matrix of the patellar tendon and that intrinsic fibroblasts prohibit the detrimental effect of stress enhancement on mechanical properties of the patellar tendon.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Tomita, F.: "Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction"Arthroscopy. 17. 461-476 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tohyama, H.: "The effect of increased stress on the patellar tendon"J. Bone Joint Surg.. 84[Br]. 184-188 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamazaki, S.: "The effect of graft-tunnel diameter disparity on intraosseous healing of the flexor tendon graft in anterior cruciate ligament reconstruction"Am. J. Sports Med.. 30. 498-505 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Numazaki, H.: "The effect of initial graft tension in anterior cruciate ligament reconstruction on the mechanical behaviors of the femur-graft-tibia complex during cyclic loading"Am. J. Sports Med.. 30. 800-805 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai, T.: "Effects of combined administration of transforming growth factor-betal and epidermal growth factor on properties of the in situ frozen anterior cruciate ligament in rabbits"J. Orthop. Res.. 20. 1345-1351 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kimura, S.: "Implantation sites and fiber diameters affect the rate of degradation in absorbable polydioxane fibers"Arthroscopy. 19. 68-74 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hara, N.: "Effects of stress deprivation on mechanical properties of the in situ frozen-thawed semitendinosus tension in rabbits"Clin Biomechanics. 18. 60-68 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Azuma, H.: "Timing of administration of transforming growth factor-beta and epidermal growth factor influences the effect on material properties of the in situ frozen-thawed anterior cruciate ligament"J Biomechanics. 36. 373-381 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tohyama, H.: "Anterior drawer test for acute anterior talofibular ligament injuries of the ankle. How much load should be applied during the test?"Am. J. Sports Med.. 31. 226-232 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tohyama, H.: "The changes in mechanical properties of regenerated and residual tissues in the patellar tendon after removal of its central portion"Clinical Biomechaniocs. 23(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tomita, F.: "Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction"Arthroscopy. 17. 461-476 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tohyama, H.: "The effect of increased stress on the patellar tendon"J. Bone Joint Surg. 84[Br]. 184-188 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamazaki, S.: "The effect of graft-tunnel diameter disparity on intraosseous healing of the flexor tendon graft in anterior cruciate ligament reconstruction"Am. J. Sports Med.. 30. 498-505 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Numazaki, H.: "The effect of initial graft tension in anterior cruciate ligament reconstruction on the mechanical behaviors of the femur-graft-tibia complex during cyclic loading"Am. J. Sports Med.. 30. 800-805 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai, T.: "Effects of combined administration of transforming growth factor-beta1 and epidermal growth factor on properties of the in situ frozen anterior cruciate ligament in rabbits"J. Orthop. Res.. 20. 1345-1351 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kimura, S.: "Implantation sites and fiber diameters affect the rate of degradation in absorbable polydioxane fibers"Arthroscopy. 19. 68-74 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hara, N.: "Effects of stress deprivation on mechanical properties of the in situ frozen-thawed semitendinosus tension in rabbits"Clin. Biomechanics. 18. 60-68 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Azuma, H.: "Timing of administration of transforming growth factor-beta and epidermal growth factor influences the effect on material properties of the in situ frozen-thawed anterior cruciate ligament"J. Biomechanics. 36. 373-381 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tohyama, H.: "Anterior drawer test for acute anterior talofibular ligament injuries of the ankle. How much load should be applied during the test?"Am. J. Sports Med.. 31. 226-232 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tohyama, H.: "The changes in mechanical properties of regenerated and residual tissues in the patellar tendon after removal of its central portion"Clin. Biomechanics. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 遠山晴一: "前十字靭帯損傷治療に対する生体力学的アプローチ"Monthly Book Orthopaedics. 13(10). 20-27 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 遠山晴一: "膝靭帯再建術のバイオメカニクス-移植腱に加える張力の重要性-"関節外科. 21(10). 155-160 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 遠山晴一: "ヒアルロン酸製剤(関節機能改善剤)をどう用いるか スポーツ外傷への応用"医薬ジャーナル. 38(10). 216-220 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 内田尚哉: "除負荷がラット膝蓋腱の力学的特性とTransforming Growth Factor-βの発現に与える効果に関する解析"日本膝関節学会誌. 26. 184-188 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 沼崎広法: "移植腱初期張力が前十字靭帯再建術における大腿骨-移植腱-脛骨複合体の力学的挙動に与える効果-繰り返し負荷試験を用いた生体力学的検討-"日本膝関節学会誌. 26. 238-242 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 安田和則: "後十字靭帯温存型セラミックス製人工膝関節の開発コンセプトとその2〜8年成績"臨床整形外科. 37. 961-969 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 近藤英司: "PDGF-BBの投与が損傷を受けた膝前十字靭帯の力学特性に与える影響"日本臨床バイオメカニクス学会誌. 23. 19-24 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 工藤敏治: "前十字靭帯再建術における屈筋腱を用いたBone Mulch ScrewとWasherLoc固定法の繰り返し負荷試験による生体力学的評価"日本臨床バイオメカニクス学会誌. 23. 209-214 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tohyama, H.: "The effect of increased stress on the patellar tendon"J. Bone Joint Surg.. 84[Br]. 440-446 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamazaki, S.: "The effect of graft-tunnel diameter disparity on intraosseous healing of the flexor tendon graft in anterior cruciate ligament reconstruction"Am. J. Sports Med.. 30. 498-505 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Numazaki, H.: "The effect of initial graft tension in anterior cruciate ligament reconstruction on the mechanical behaviors of the femur-graft-tibia complex during cyclic loading"Am. J. Sports Med.. 30. 800-805 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tohyama, H.: "Avulsion fracture at the femoral attachment of the anterior cruciate ligament after intercondylar eminence fracture of the tibia"Am. J. Sports Med.. 30. 279-283 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakai, T.: "Effects of combined administration of transforming growth factor-betal and epidermal growth factor on properties of the in situ frozen anterior cruciate ligament in rabbits"J. Orthop. Res.. 20(6). 1345-1351 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kimura, S.: "Implantation sites and fiber diameters affect the rate of degradation in absorbable polydioxane fibers"Arthroscopy. 19(1). 68-74 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hara, N.: "Effects of stress deprivation on mechanical properties of the in situ frozen-thawed semitendinosus tension in rabbits"Clin Biomechanics. 18. 60-68 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tohyama, H.: "Anterior drawer test for acute anterior talofibular ligament injuries of the ankle"Am. J. Sports Med.. 31(In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tohyama, H.: "The changes in mechanical properties of regenerated and residual tissues in the patellar tendon after removal of its central portion g"Clin Biomechanics. 19(In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomita F.: "Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction"Arthroscopy. 17. 461-476 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamazaki S.: "The effect of graft-tunnel diameter disparity on intraosseous healing of the flexor tendon graft in anterior cruciate ligament reconstruction"The American Journal of Sports Medicine. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Tohyama H.: "The effect of stress enhancement on the patellar tendon autograft. An experimental study with the in situ frozen-thawed tendon"Journal of Bone and Joint Surgery [British volume]. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Numazaki H.: "The effect of initial graft tension on mechanical behaviors of the femur-graft-tibia complex with anterior cruciate liament reconstruction during cyclic loading"The American Journal of Sports Medicine. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 内田尚哉: "除負荷がラット膝蓋腱の力学的特性とTransforming Growth Factor-βの発現に与える効果に関する解析"日本膝関節学会誌. 26. 184-188 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 遠山晴一: "線維芽細胞壊死後の膝蓋腱におけるIII型collagenの発現に関する分子生物学的および免疫組織学的検討"日本膝関節学会誌. 26. 86-91 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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