| Project/Area Number |
13671507
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
HASHIMOTO Jun Osaka University Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (40237938)
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| Co-Investigator(Kenkyū-buntansha) |
MYOUI Akira Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (10263261)
NAKASE Takanobu Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (00283755)
TORITSUKA Yukiyoshi Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (20324775)
KANEDA Yasushi Osaka University Graduate School of Medicine, Professor, 医学系研究科, 教授 (10177537)
YOSHIKAWA Hideki Osaka University Graduate School of Medicine, Professor, 医学系研究科, 教授 (60191558)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | joint contracture / immobilization / proliferation of synoviocyte / transcription factor / decoy / E2F / HVJ-envelope / gene therapy / HVJ-evelopeベクター |
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| Research Abstract |
Joint contracture followed by prolonged joint immobilization is a serious problem in orthopaedic treatment. The cause of that was reported as that proliferation of fibrous synovial tissue in the joint cavity was characteristic. To prevent the contracture followed by immobilization, we planned to suppress the proliferation of synoviocytes by gene transfer of transcription factor E2F decoy into synoviocytes. As the animal model of joint immobilization, we used Wilson's rat model (Wilson et al Clin Orthop 1988). At 1, 2, 3 and 4 weeks after joint immobilization, we examined the Hematoxylin and eosin staining of the sagittal section of knee joint and confirmed the proliferation of synovial tissue and adhesion of extracellular matrix in the joint reproductively. In vitro study, we examined the semi-quantitative transfection efficiency of gene transfer into fibroblast-like cell line (3Y1-B and NRK) and primary generated rat synoviocyte using FITC-labeled oligonucleotide (FITC ODN) included in HVJ-envelope vector, and determined the optimal condition of gene transfer. In vivo, we observed the high efficient gene transfer into synovial tissue by intraarficular injection of FITC ODM included in HVJ envelope vector, and almost no transfer into other tissue such as articular cartilage, meniscus and ligament. Now we examine in vivo gene transfer using E2F decoy included in HVJ envelope and analyze the proliferation of synovial tissue and production of extracellular matrix by molecular biologically and histologically.
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