Biophysic study of maturation and strength of bone induced by callotasis
| Project/Area Number |
13671515
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | THE UNIVERSITY OF TOKUSHIMA |
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Principal Investigator |
TAKATA Shinjiro The University of Tokushima, University Hospital, Assistant Professor, 医学部附属病院, 講師 (20284292)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Akira The University of Tokushima, Faculty of Pharmaceutical Science, Associate Professor, 薬学部, 助教授 (40035556)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | callotasis / bone mineral metabolism / Fourier transform infrared spectroscopy / solid-state NMR / femur / bone strength / Pqct / コラーゲン / 熱分析法 / 固体NMR |
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| Research Abstract |
We studied maturation and strength of bone during distraction osteogenesis in callotasis. Under general anesthesia, a longitudinal skin incision was made of the lateral aspect of the femur to expose femoral shaft. Four self -tapping screws were placed at right angles to the longitudinal axis of the femoral shaft. The screws were clamped to the external fixator. After that, transverse osteotomy was done between 3rd and 4th screws using a hand saw. Distraction started 7 days after operation at a rate of 0.25 mm every 12 hours (0.5mm/day) for 21 days. Biophysic analysis, measurement of bone strength and bone mineral density were performed just after 21 days of distraction, 3 weeks, 6 weeks, 9 weeks, 12 weeks, 15 weeks after distraction.
The results obtained in this study showed that bone strength, bone mineral density of both cancellous and cortical bones, periosteal circumference increased with time during callotasis. As for biophysic changes, T1 relaxation time mesured by phosphorus-31 solid-state nuclear magnetic resonance (NMR) increased after 21 days of distraction, which means an increase in bone strength. In addition, mineral to matrix ratio measured by Fourier transform infrared spectroscopy (FTIR) increased during distraction, which suggests that mineral to matrix ratio may be one of important indices of strength and maturation of bone in distraction osteogeneis.
Based on these facts, T1 relaxation time by phosphrus-31 solid state NMR and mineral to matrix ratio by FTIR become promising predictors of maturation and strength of bone during distraction osteogenesis.
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Report
(3 results)
Research Products
(12 results)
