Study of the molecular pathology of ADPKD

• Project/Area Number: 13671638
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kyorin University (2002); The University of Tokyo (2001)
• Principal Investigator: HORIE Shigeo Kyorin University, School of Medicine, Associate Professor, 医学部, 助教授 (40190243)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥4,100,000 (Direct Cost: ¥4,100,000); Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000); Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
• Keywords: Pkd1 / ADPKD / Knock-out mice / microarray / polycystin1 / PKD1 / Wnt / C-MYC

Research Abstract

We analyzed the comprehensive gene expression analysis of Pkd1^%, using cDNA microarray. We extracted the mRNA from E14.5 fetus kidney, and applied mRNA onto DNA chip containing more than 3000 genes. We identified that POLD1 (DNA polymerase delta catalytic subunit-1) was up-regulted in null mice. Interestingly, apoptosis driving genes such as caspase 1, 7, 11 and IL-1b, Transcription factors such as GATA2, Lbx, 1 and jun D1, a cell cycle related gene were down-regulated in null mice. Based on these data, renal cystogenesis involved the defected apoptosis pathway and stimulated cell cycle turnover.

Research Products

• [Publications] Muto S, Horie S, et al.: "Pioglitazone improves the phenotype and molecular defects of a targeted Pkd1 mutant"Hum Mol Genet.. 11(15). 1731-1742 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Muto S., Horie S., et al.: "Pioglitazone improves the phenotype and molecular defects of a targeted Pkd1 mutant"Hum Mol Genet. 11(15). 1731-1742 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Muto S, et al.: "Pioglitazone improves the phenotype and molecular defects of a targeted Pkd1 mutants"Human Molecular Genetics. 11. 1731-1742 (2002)