Identification of genes associated with relapse of prostate cancer and gene therapy

Research Project

Project/Area Number	13671640
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Urology
Research Institution	Kanazawa university
Principal Investigator	MIZOKAMI Atsushi University Hospital assistant, 医学部附属病院, 助手 (50248580)
Co-Investigator(Kenkyū-buntansha)	KASHIDA Kiyoshi Obstetrics and Gynecology assistant professor, 医学系研究科, 助教授 (70186667) NAMIKI Mikio Obstetrics and Gynecology professor, 医学系研究科, 教授 (70155985)
Project Period (FY)	2001 – 2002
Project Status	Completed (Fiscal Year 2002)
Budget Amount *help	¥3,600,000 (Direct Cost: ¥3,600,000) Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000) Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywords	prostate cancer / androgen dependency / relapse / COX-2 / 遺伝子治療
Research Abstract	We inoculated LNCaP cells, androgen-sensitive human prostate cancer cells, onto 5-week aged scid mice subcutneously with 50 % matrigel. After tumor was increased visually, we castrated the mouse. First, tumor retarded for 2 weeks. Then it stated to enlarge. We sacrificed the mouse and took the tumor and subcultured. (LN-RECO). Next we inoculated the LN-RECO to the castrated mouse. Again we took the enlarged tumor and subcultured (LN-REC4). We characterized LN-REC4 cells. Although LN-REC4 cells were stimulated by androgen in vitro, the growth speed of those cells in the castrated mouse was faster than LNCaP cells, suggesting that LN-REC4 seemed to be a good model that reflect the relapse of prostate cancer. We also investigated the COX-2 gene since this gene seemed to be related with progression of prostate cancer. Overexpression of COX-2 gene in LNCaP cells induced cell proliferation in vitro and in vivo, and induced VEGF secretion.