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Searching of factors facilitate spermatogonial stem cell proliferation (Investigation with transplantation and in vitro analysis)

Research Project

Project/Area Number 13671663
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionYOKOHAMA CITY UNIVERSITY

Principal Investigator

OGAWA Takehiko  Yokohama City University School of Medicine lecturer, 医学部, 講師 (50254222)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsspermatogenesis / spermatogonia / transplantation / stem cell
Research Abstract

1) Fertilization of recipient mice with donor germ cells
Pup mice, aged 8 〜 20 days old, were treated with regional radiation to eliminate innate testis germ cells. Spermatogonial transplantation was performed on those mice. Donor spermatogenesis took place in the host testes pervasively at 1 〜 2 months later. Mating with regular females produced donor germ cell derived progeny. This result indicates that spermatogonial stem cells in the pup testis expanded more rapidly than when they were transplanted in the about recipients.
2) GnRH analogue effects on spermatogonial cell proliferation
We analyzed proliferation of spermatogonia using transplantation and confocal laser microscopic analysis. In GnRH analogue treated mice, the number of spermatogonia significantly increased compared to those in control mice, indicating that GnRH analogue enhanced proliferation or suppressed apoptosis of the spermatogonia. Another experiment using S1/S1^d mice as recipients showed that the effect of GnRH analogue is independent from SCF signal.
3) Culture of spermatogonial stem cells
Testis cells of GFT mice were cultured for 2 to 3 weeks. To confirm the presence of spermatogonial stem cells, they were transplanted to the testes of pup mouse. Several different culture conditions were tested, including feeder cells (STO, 15P-1), coated dishes with lamina or collagen, madrigal matrix, and so on. Among them cultured cells on the madrigal matrix showed they contained spermatogonial stem cells for such periods.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Udagawa K et al.: "GmRH analogue, leuprorelin acetate, promotes regeneration of rat spermatogenesis after severe chemical damage"International Journal of Urology. 8. 615-622 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Udagawa K et al.: "Recovery of spermatogenesis by high dose gonadotropin-releasing hormone analogue treatment in rat cryptorchid testis after orchidopexy"Journal of Urology. 168. 1279-1283 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小川毅彦: "精原細胞を用いた応用"分子細胞治療. 1. 412-415 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogawa T et al.: "Expansion of murine spermatogonial stem cells through serial transplantation"Biology of Reproduction. 68. 316-322 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohmura M et al.: "Increment of murine spermatogonial cell number by gonedotropin-releasigng hormone analogue is independent of stem cell factor-c-kit signal"Biology of Reproduction. (in print).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Udagawa K, Ogawa T, Watanabe T, Yumura Y, Takeda M, Hosaka M: "GnRH analogue, leuprorelin acetate, promotes regeneration of rat spermatogenesis after severe chemical damage."Int J Urol. 8. 615-622 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Udagawa K, Takeda M, Hosaka M, Kubota Y, Ogawa T: "Recovery of spermatogenesis by high dose gonadotropin-releasing hormone analogue treatment in rat cryptorchid testes after orchiopexy."J Urol.. 168. 1279-1283 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogawa T: "Application of spermatogonia"Bunshi Saibou Chiryo. 1. 412-415 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogawa T, Ohmura M, Yumura Y, Sawada H, Kubota Y: "Expansion of murine spermatogonial stem cells through serial transplantation."Biol Reprod. 68. 316-322 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohmura M, Ogawa T, Ono M, Dezawa M, Hosaka M, Kubota Y, Sawada H.: "Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor-c-kit signal."Biol Reprod.[epub ahead of print]. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Udagawa K, Ogawa T, et al.: "GnRH analogue, leuprorelin acetate, promotes regeneration of rat spermatogenesis after severe chemical damage"Int J Urol. 8・11. 615-622 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Udagawa K, Ogawa T, et al.: "Recovery of Spermatogenesis by High dose gonadotropin-releasing hormone analogue treatment in rat cryptorchid testis after orchiopexy"J Urol. 168・3. 1279-1283 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ogawa T, et al.: "Expansion of Murine Spermatogonial Stem Cells through Serial Transplantation"Biol Reprod. 68・1. 316-322 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohmura M, Ogawa T, et al.: "Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor -c-kit signal"Biol Reprod. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 小川毅彦: "精原細胞移植-男性不妊症への応用-"泌尿器科外科. 14 suppl. 423-425 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Dobrimski, Ogawab T, et al.: "Effect of the GmRH-agonist leuprolide on colonization of rocipient taites"Tissue Cell. 33・2. 200-207 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogawa T: "Spermatogovial trdurplantction : the principle and possible applications"J Mol Med. 79・7. 368-374 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Llolagawa K, Ogawa T, et al.: "GmRH analogue, leuprorclin acetate, promotes regenerarion of rat spermatogenesis after severe chemical damage"Int J Llrol. 8・11. 615-622 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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