• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Active specific immunotherapy with MN\CA IX antigen peptide vaccines for renal cell carcinoma

Research Project

Project/Area Number 13671671
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionNara Medical University

Principal Investigator

UEMURA Hirotsugu  Dept. Urology, Nara Med. University, Assit. Professor, 医学部, 講師 (90213397)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Kazuhiro  2^<nd> Dept. Pathology, Aichi Med. University, Assit. Professor, 医学部, 講師 (60109759)
TSUNODA Takuya  Institute of Medical Science, Tokyo University, Assit. Professor, 医科学研究所, 講師 (30275359)
CHO Masaki  Dept. Urology, Nara Med. University, Stuff, 医学部, 助手 (90285362)
岡島 英二郎  奈良県立医科大学, 医学部, 助手 (70326346)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsrenal cell carcinoma / MN / CA9 / antigen peptide / cancer vaccine
Research Abstract

MN/CA IX antigen is a tumor-associated antigen expressed in approximately 90 % of renal cell carcinomas (RCQ). We have synthesized 9mer antigen peptides restricted to HLA-A24 and employed them as tumor vaccines to investigate the ability to induce this antigen specific responses in mouse syngeneic ROC model. Antigen specific CTL was induced by immunization of MN/CA9 9mer peptide vaccine in mouse system. This finding suggests that vaccination with MN/CA9 antigen peptides may be potential therapeutic approach for RCC patients. We also have investigated the capacity of CTL induction using RCC patient lymphocytes in vitro. In addition, presence of CTL precursor was investigated using PBMC from the patients with metastatic RCC patients. Stimulation of patient lymphocytes with autologons dendritic cell loaded MN/CA9 peptides resulted in antigen specific CTL induction.
Based on these pie-clinical data, we started a phase-I study to investigate MN/CA9 peptide vaccines by subcutaneous administration in patients with disseminated renal cell carcinoma since July 2002. Patients with distant metastases received three sets of MN/CA9 9-mer peptide vaccines 6 times at 2-week intervals. Primary end points of this study are to evaluate the toxicity and immunogenicity of these antigen peptide vaccines. Six patients finished the protocol until now and only low grade toxicities such as fever, pruritus and local reaction (swelling, pain) were observed. Antigen specific cell-mediated cytotoxicity was induced in some patients. In addition, antibodies (IgG) against MN/CA9 peptides vaccines were detected in some patients. These findings suggest that our MN/CA9 peptide vaccines may be safe and applicable for HLA-A24 positive RCC patients. Moreover; we are currently investigating the generation of modified peptide vaccines to obtain more powerful antigenicity.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Y Hirano, K Fujimot, M Yoshii, N Tanaka, Y Hayashi, H Momose, S Samma, E Okajima, H Uemura, et al.: "Non-ischemic Nephron-sparing Surgery for Small Renal Cell Carcinoma : Complete Tumor Enucleation Using a Microwave Tissue Coagulator"Jpn J Clin Oncol. 32・3. 95-102 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K Gramaier, M Deweijert, H Uemura, J Schalken, E Oosterwijk: "Renal cell carcinoma-associated methylation and expression : in vivo and in vitro studies"Urology. 60. 357-362 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 植村天受, 平尾佳彦: "流血中腎癌細胞の検出方法と臨床的意義"癌と化学療法. 29・10. 1712-1718 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M Cho, H Uemura, S-C Kim, Y Kawada, K Yoshida, Y Hirao, N Konishi, S Saga K Yoshikawa: "Hypomethylation of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma"British Journal of Cancer. 85. 563-567 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 植村天受: "腎細胞癌の新しいバイオマーカーMN/CA9"臨床泌尿器科. 55・5. 329-335 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Kawada, M Nakamura, E Ishii, K Shimada, E Oosterwijk, H Uemura, Y Hirano, SC Kim, N Konishi: "Aberrations of the p14^<ARF> and p16^<INK4a> genes in renal cell"Jap J Cancer Res. 92. 1293-1299 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Hirao, K. Fujimoto, M. Yoshii, N. Tanaka, Y. Hayashi, H. Momose, S. Samma, E. Okajima, H. Uemura, et al.: "Non-ischemic nephron-sparing Surgery for Small Renal Cell Carcinoma: Complete Tumor Enucleation Using a Microwave Tissue Coagulator"Jpn J Clin Oncol.. 32. 95-102 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Gramaier, M. Deweijert, H. Uemura, J. Schalken, E. Oosterwijk: "Renal cell carcinoma associated methylation and expression: in vivo and in vitro studies"Urology. 60. 357-362 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Uemura and Y. Hirao:: "Clinical significance for circulating cancer cells in renal cell carcinoma"Jpn J Cancer Chmother. 29. 1712-1718 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Cho, H. Uemura, S-C Kim, Y. Kawada, K. Yoshida, Y. Hirao, N. Konishi, S. Saga and K. Yoshikawa:: "Hypomethylation of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma"British journal of Cancer. 85. 563-567 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Uemura:: "MN/CA9 Novel biomarker for renal cell carcinoma"Rinsho-Hinyokika. 55. 329-335 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Kawada, M. Nakamura, E. Ishii, K. Shimada, E. Oosterwijk, H. Uemura, Y. Hirao, SC Kim, N. Konishi: "Aberrations of the p14^<ARF>and p16^<INK4a>genes in renal cell"Jap J Cancer Res. 92. 1293-1299 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Hirao, K Fujimoto, M Yoshii, N Tanaka, Y Hayashi, H Momose, S Samma, E Okajima, H Uemura, et al.: "Non-ischemic Nephron-sparing Surgery for Small Renal Cell Carcinoma: Complete Tumor Enucleation Using a Microwave Tissue Coagulator"Jpn J Clin Oncol. 32・3. 95-102 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K Gramaier, M deweijert, H Uemura, J Schalken: "Renal cell carcinoma-associated methylation and expression: in vivo and in vitro studies"Urology. 60. 357-362 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 植村天受, 平尾佳彦: "流血中腎癌細胞の検出方法と臨床的意義"癌と化学療法. 29・10. 1712-1718 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M Cho, H Uernura, S-C Kim, K Yoshida, Y Hirao, et al.: "Hypomethylatioin of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma"British Journal of Cancer. 85:563-567,2001. 85. 563-567 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y Kawada, M Nakamura, K Shimada, H Uemura, Y Hirao, et al.: "Aberrations of the p14^<ARF> and pl6^<INK4a> genes in renal cell carcinomas"Japanese Journal of Cancer. 92:1293-1299,2001. 92. 1293-1299 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 仲川嘉紀, 植村天受, 清水一宏, 趙順規, 吉川元祥, 平尾佳彦 他: "泌尿器科腫瘍学にお伐る分子研究の展望:腎細胞癌の発癌・転移とMN/CA9"泌尿器科紀要. 47. 809-814 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 植村天受: "腎細胞癌の新しいバイオマーカーMN/CA9"臨床泌尿器科. 55. 329-335 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi