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PROMOTING EFFECTS OF JUVENILE ESTROGEN TREATMENT ON DEVELOPMENT OF AUTOIMMUNE PROSTATITIS IN NEONATALLY THYMECTOMIZED MICE

Research Project

Project/Area Number 13671684
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionAICHI CANCER CENTER RESEARCH INSTITUTE

Principal Investigator

HAYASHI Norio  AICHI CANCER CENTER RESEACH INSTITUTE RESARCHER, 研究所, 研究員 (70198852)

Co-Investigator(Kenkyū-buntansha) TAGUCHI Osamu  AICHI CANCER CENTER RESEACH INSTITUTE, DIVISION OF MOLECULAR PATHOLOGY SECTION HEAD, 分子病態学部, 室長 (00142167)
SUGIMURA Yoshiki  AICHI CANCER CENTER RESEACH INSTITUTE RESEARCHER, 研究所, 研究員 (90179151)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsESTROGEN / PROSTATE / MOUSE / THYMECTOMY / PROSTATITIS / AUTO IMMUNE / NEONATAL
Research Abstract

Neonatal treatment of C57BL/6 male mice with estradiol-17β (E_2) (8mg/kg body weight) induces prostate epithelial hyperplasia and/or dysplasia with inflammatory cell infiltration. Five-consecutive-treatment from the day of birth (E_2-0)induced lesions in all mice. When the treatment was started on day 7 after birth (E_2-7), similar epithelial abnormalities with a lesser exert of inflammatory cell infiltration were caused in about the half mice. Few prostatic lesions were found when E_2 injection was started on day 14 (E_2- 14). Autoimmune prostatitis developed spontaneously in approximately 35% of C57BL/6 mice after thymectomy (Tx) on day 3 (Tx-3) after birth. When Tx-3 mice received E_2 injections from day 7 (Tx-3 + E_2-7) or day 14 (Tx-3 + E_2-14) but not day 21 (Tx-3 + E_2-21), a high incidence (around 80%) of severe prostatitis developed. Injections of testosterone propionate (TP) were without effect, even when given at young age. Autoantibodies against prostate epithelial cells were detected in the sera of Tx-3 mice with prostatitis, irrespective of whether given E_2 injections but never neonatal E_2 exposure alone. Transplantation of newborn prostate under the kidney capsules of Tx-3 or Tx-3 + E_2-14 mice with prostatitis evoked intense inflammation around the grafts. Such an inflammatory reaction was never seen with transplants into the E_2-0 mice. Development of prostatitis in Tx-3 and Tx-3 + E_2-14 but not E_2-0 mice could be prevented by the intraperitoneal injection of spleen cells from normal male mice at day 7. We conclude that effector belongs to the CD4^+CD25^+ class of lymphocytes.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Ishii, K., et al.: "Aminopeptidase N regulated by zinc in human prostate participates in tumor cell invasion"Int J Cancer. 92. 49-54 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishii, K., et al.: "Decreases of metallothionein and aminopeptidase N in renal cancer tissues"J Biochem. 129. 253-258 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sugimura, Y., et al.: "An anterior urethral stitch improves urinary incontinence following radical prostatectomy"Int J Urol. 8. 153-157 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue, T., et al.: "Preoperative predictors of cancerous involvement of the neurovascular bundles in patients with localized prostate cancer"Int J Urol. 9. 47-53 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, S., et al.: "Down-regulation of Lsm1 is involved in human prostate cancer progression"Br J Cancer. 86. 940-946 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tei, K., et al.: "Roles of cell adhesion molecules in tumor angiogenesis induced by co-transplantation of cancer and endothelial cells to nude rats"Cancer Res. 62. 6289-6296 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishii, K., Usui, S., Sugimura, Y., Yoshida, S., Hioki, T., Tatematsu, M., Yamamoto, H., Hirano, K.: "Aminopeptidase N regulated by zinc in human prostate participates in tumor cell invasion"Int J Cancer.. 92. 49-54 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishii, K., Usui, S., Sugimura, Y., Yamamoto, H., Yoshikawa, K., Hirano, K.: "Inhibition of aminopeptidase N (AP-N) and urokinase-type plasminogen activator (uPA) by zinc suppresses the invasion activity in human urological cancer cells"Biol Pharm Bull.. 24. 226-230 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishii, K., Usui, S., Sugimura, Y., Yamamoto, H., Yoshikawa, K., Hiran, K.: "Extract from Serenoa repens suppresses the invasion activity of human urological cancer cells by inhibiting urokinase-type plasminogen activator"Biol Pharm Bull. 24. 188-190 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishii, K., Usui, S., Yamamoto, H., Sugimura, Y, Tatematsu, M., Hirano, K.: "Decreases of metallothionein and aminopeptidase N in renal cancer tissues"J Biochem.. 129. 253-258 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sugimura, Y., Hioki, T., Yamada, Y, Fumino, M., Inoue, T.: "An anterior urethral stitch improves urinary incontinence following radical prostatectomy"Int J Urol.. 8. 153-157 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kontani, K., Taguchi, O., Narita, T., Izawa, M., Hiraiwa, N., Zenita, K., Takeuchi, T., Murai, H., Miura, S., Kannagi, R.: "Modulation of MUC1 mucin as an escape mechanism of breast cancer cells from autologous cytotoxic T-lymphocytes"Br. J. Cancer. 84. 1258-1264 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirose, F., Ohshima, N., Shiraki, M., Inoue, Y. H., Taguchi, O., Nishi, Y., Matsukage, A. and Yamaguchi, M.: "Ectopic expression of DREF induces DNA synthesis, apoptosis, and unusual morphogenesis in the drosophia eye imaginal disc : possible interaction with polycomb and trithorax group proteins"Mol. Cell. Biol.. 21. 7231-7242 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue, T., Hioki, T., Hayashi, N., Takahashi, S., Shirai, T., Sugimura, Y.: "Preoperative predictors of cancerous involvement of the neurovascular bundles in patients with localized prostate cancer"Int J Urol.. 9. 47-53 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, S., Suzuki, S., Inaguma, S., Cho, Y.M., Ikeda, Y., Hayashi, N., Inoue, T., Sugimura, Y., Nishiyama, N., Fujita T, Ushijima, T., Shirai, T.: "Down-regulation of Lsm1 is involved in human prostate cancer progression"Br J Cancer.. 86. 940-946 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kontani, K., Taguchi, O., Ozaki, Y., Hanaoka, J., Tezuka, N., Sawai, S., Inoue, S., Fujino, S., Maeda, T., Itoh, Y., Ogasawara, K., Sato, H., Ohkubo, I., Kudo, T.: "Novel vaccination protocol consisting of injecting MUC1 DNA and non-primed dendritic cells at the same region greatly enhanced MUC1 -specific anti-tumor immunity in a murine model"Cancer Gene Ther.. 9. 330-337 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tei, K., Kawakami-Kimura, N., Taguchi, O., Kumamoto, K., Higashiyama, S., Taniguchi, N., Toda, K., Kawata, R., Hisa, Y., and Kannagi, R.: "Roles of cell adhesion molecules in tumor angiogenesis induced by co-transplantation of cancer and endothelial cells to nude rats"Cancer Res.. 62. 6289-6296 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kontani, K. et al.: "Novel vaccination protocol consisting of injecting MUC1 DNA and non-primed dendritic cells at the same region greatly enhanced MUCI-specific anti-tumor immunity in a murine model"Cancer Gene Ther.. 9. 330-337 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tei, K.et al.: "Roles of cell adhesion molecules in tumor angiogenesis induced by co-transplantation of cancer and endothelial cells to nude rats"Cancer Res.. 62. 6289-6296 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sugimura, Y. et al.: "An anterior urethral stitch improves urinary incontinence following radical prostatectomy"Int.J.Urol.. 8. 153-157 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirose, F. et al.: "Ectopic expression of DREF induces DNA synthesis, apoptosis, and unusual morphogenesis in the dorosophia eye imaginal disc"Mol.Cell.Biol.. 21. 7231-7242 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kontani, K., Taguchi, O.et al.: "Novel vaccination protocol consisting of injecting MUC1 DNA and non-primed dendritic cells at the same region greatly enhanced MUC-specific anti-tumor immunity in a murine model"Cancer Gene Ther. (in press).

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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