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ORALLY ADMINISTERED HPV 16 VACCINE STIMULATING GUT-ASSOCIATED LYMPHOID TISSUES

Research Project

Project/Area Number 13671701
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKanazawa University

Principal Investigator

SASAGAWA Toshiyuki  Kanazawa University, Faculty of Medicine, 医学部, 助教授 (30272975)

Co-Investigator(Kenkyū-buntansha) HAMA Yuko  Asahi Glass Corp. Central Laboratory, 中央研究所, 主幹研究員
SHIMAKAGE Misuzu  Osaka National Hospital, Clinical Research, 臨床研究部, 研究員
INOUE Masaki  Kanazawa University, School of Medicine, 大学院・医学系研究科, 教授 (10127186)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsHuman pgpillomavirus / Prophylactic vaccine / Virus-like particles / Cervical cancer / Oral vaccine / Mouse model system / Cholera Toxin / 診断法 / 分子疫学
Research Abstract

Human papillomavirus type16 (HPV16) is the most important risk factor for cervical cancer. Thus, effective prophylactic vaccine against HPV 16 is expected for prevention of cervical cancer development, which is the secondary common cancer of women worldwide.
Recent study showed that nasal administration of HPV16 virus-like particles (VLP) is able to elicit mucosal antibody neutralizing HPV 16. In the present study, we tested an efficacy of orally administered HPV16 vaccine using yeast synthesizing HPV 16 VLP (HPV 16 VLP-yeast). Preliminary experiments showed that freeze dried yeast was good candidate for delivering antigen to the gut and stimulating the gut-associated lymphoid tissues (GALT), since it was not digested in the stomach but digested in the intestine.
Positive control mice were nasally administered with purified HPV16 VLP (Nasal-Ad), and the others were orally administered with freeze dried yeast (Oral-Ad). Two mice were orally administered with control yeast, two mice with HPV6 VLP-yeast, 6 mice with HPv16 VLP-yeast, and 12 mice with HPV16 VLP-yeast and Cholera toxin (CT). HPV-specific antibody responses were tested with HPV6- and 16-VLP-ELISA. Two Nasal-Ad mice, 3 of 6 Oral-Ad mice with HPV16 VLP-yeast, and 4 of 12 Oral-Ad mice with HPV 16 VLP-yeast + CT elicited HPV 16-specific IgA and IgG serum antibody (Ab), whereas mice administered with control yeast or with HPV6-VLP-yeast had no such responses.
The liters of the serum Ab were as high as those in the mice nasally administered with HPV16 VLP, and no difference in serum Ab titers was observed by administrating CT or not. Weak but HPV 16-specifc IgA responses were detected in vaginal secretions of 2 of 12 Oral-Ad mice with CT. Now we are optimizing the condition able to induce stronger mucosal IgA response.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 笹川寿之, 井上正樹: "妊婦の初診時に発見された子宮疾患の取り扱い:膣部コンジローマ"産婦人科の実際. 50. 1159-1166 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 笹川寿之: "ヒトパピローマウイルス感染症"思春期学別刷. 19. 301-308 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 笹川寿之: "HPV感染と細胞診"産婦人科の治療. 85. 27-34 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Sasagawa, et al.: "High-risk and multiple HPV infection associated with cervical abnormalities in Japanese women"Cancer Epidemiol. Biomerk. Prev.. 10. 45-52 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Yamazaki, T.Sasagawa et al.: "Hybrid capture-II and LCR-E7 PCR assays for HPV typing in cervical cytological samples"Int. J. Cancer. 94. 222-227 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Sasagawa, et al.: "Mucosal immunoglobulin A and-G responses to oncogenic human papilloma virus capsids"Int J Cancer. 1. 324-335 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 笹川寿之: "成人看護学 病態生理・疾病論(II)改訂版"女性生殖器. 24 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Minemoto, J. Gannon, M. Masutani, H. Nakagama, T. Sasagawa, M. Inoue, Y. Masamune and K. Yamashita.: "Characterization of Adriamycin-induced G2-Arrest and Its Abrogation by Caffeine in FL-amnion Cells with or without p53"Exp. Cell Res.. 262. 37-48 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Sasagawa, W. Basha, H. Yamazaki, M. Inoue.: "High-risk and multiple HPV infection associated with cervical abnormalities in Japanese women"Cancer Epidemiol. Biomerk. Prev.. 10. 45-52 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Shimakage, T. Sasagawa.: "Detection of Epstein-Barr virus-determined nuclear antigen-2 mRNA hybridization"J. Virological Methods. 93. 23-32 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Shimakage, T. Sasagawa.: "Expression of Epstein -Barr virus in cutaneous T-cell Lymphoma mycosis fungoides"Int J Cancer. 92. 26-231 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Higashi, T. Sasagawa, M. Inoue, R. Oka, L. Shuangying, K. Saijoh.: "Overexpression of latent transforming growth factor-_1(TGF-_1) binding protein in association with TGF-_1 in ovarian carcinoma"Jpn. J. Cancer Res.. 92. 506-515 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] R. Oka, T. Sasagawa, I. Ninomiya, K. Miwa, H. Tanii, K. Saijoh: "Reduction of the local expression of complement component 6 (C6) and 7 (C7) mRNAs in oesophageal carcinoma"Euro. J. Cancer. 37. 1158-1165 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Yamazaki, T. Sasagawa, W. Basha, T. Segawa, M. Inoue: "Hybrid capture-II and LCR-E7 PCR assays for HPV typing in cervical cytological samples"Int. J. Cancer. 94. 222-227 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimakage M, Horii K, Tempaku A, Kakudo K, Shirasaka T, Sasagawa T.: "Association of Epstein-Barr virus with oral cancers."Hum Pathol.. 33(6). 608-614 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasagawa T, Rose RC, Azar KK, Sakai A, Inoue M.: "Mucosal immunoglobulin-A and -G responses to oncogenic human papilloma virus capsids."Int J Cancer.. 1;104(3). 328-335 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Qi, B., Qi, Y., Watari, A., Yoshioka, N., Inoue, H., Minemoto, Y., Yamashita, K., Sasagawa, T. and Yutsudo, M: "Pro-apoptotic ASY/Nogo-B protein associates with ASYIP."J. Coll. Physiol.. in print.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minemoto, Y, Uchida, S., Ohtsubo, M., Shimura, M., Sasagawa. T., Hirata, M., Nakagama, H., Ishizaka, Y., and Yamashita, K.: "Loss of p53 induces M-phase retardation Following G2 DNA damage checkpoint abrogatione"Arch. Biochem. Biophys.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 笹川寿之, 井上正樹: "妊婦の初診時に発見された子宮疾患の取り扱い:膣部コンジローマ"産婦人科の実際. 50. 1159-1166 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 笹川寿之: "ヒトパピローマウイルス感染症"思春期学別刷. 19. 301-308 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 笹川寿之: "HPV感染と細胞診"産婦人科の治療. 85. 27-34 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Sasagawa, et al.: "High-risk and multiple HPV infection associated with cervical abnormalities in Japanese women"Cancer Epidemiol. Biomerk. Prev.. 10. 45-52 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Yamazaki, T.Sasagawa et al.: "Hybrid capture-II and LCR-E7 PCR assays for HPV typing in cervical cytological samples"Int. J. Cancer. 94. 222-227 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Sasagawa, et al.: "Mucosal immunoglobulin-A and -G responses to oncogenic human papilloma virus capsids"Int J Cancer. 1. 324-335 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 笹川寿之: "成人看護学病態生理・疾病論(II)改訂版"女性生殖器. 24 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 笹川 寿之: "ヒトパピローマウィルス感染症"思春期学. 19・3. 301-308 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroghi Yamazaki, Toshiyuki Sasagawa etc.: "Hybrid Capture-II and LCR-E7 PCR Assays for HPV typing in cervical cytologic samples"Int. J. Cancer. 94. 222-227 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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