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EMBRYO-DERIVED PLATELET-ACTIVATING FACTOR MODULATES ENDOMETRIAL AND DECIDUAL EXPRESSION OF ADHESION MOLECULES DURING IMPLANTATION.

Research Project

Project/Area Number 13671732
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionOITA UNIVERSITY

Principal Investigator

NARAHARA Hisashi  OITA UNIVERSITY, DEPT. OF OBSTET. & GYNECOL., PROFESSOR (60211447)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2002: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
Keywordsplatelet-activating factor / implantation / endometrium / decidua / adhesion molecule / ケモカイン
Research Abstract

Platelet-activating factor (PAF) has been implicated in a number of reproductive processes including implantation. To investigate the role of PAF in implantation, we evaluated the expression of various adhesion molecules in endometrial stromal cells (ESC) or decidual cells (DC). We measured the expression of three adhesion molecules in ESC and DC following stimulation with an analogue, carbamyl-PAF (C-PAF). This substrate is not metabolized. These three adhesion molecules are integrin α1, integrin β1, and fibronectin. Immunohystochemical analysis for these integlins and an enzyme-linked immunosorbent assay for fibronectin were used. The non-stimulated ESC constitutively expressed both integrin α1 and β1 weakly. The expression of these adhesion molecules in ESC and DC was increased by the stimulation with C-PAF. The C-PAF-induced stimulation in DC was more evident than that in ESC. C-PAF increased the production of fibronectin in ESC and DC in a dose-dependent manner. These C-PAF-induced effects were abolished following pretreatment with PAF receptor antagonists, Y-24180 and CV2086. It is suggested that the PAF secreted by the decidual tissue and by the developing embryo stimulates the expression of these adhesion molecules at the maternal-fetal interface.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All 2003 2002 2001

All Journal Article (16 results)

  • [Journal Article] Effects of colony-stimulating factors on the secretion of platelet-activating factor acetylhydrolase by human decidual macrophages2003

    • Author(s)
      Narahara H, Mine S, Kawano Y, Johnston JM, Miyakawa I
    • Journal Title

      Am J Obstet Gynecol 188(1)

      Pages: 157-161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids.2003

    • Author(s)
      Kawasaki F, Kawano Y, Kosay Hasan Z, Narahara H, Miyakawa I
    • Journal Title

      Clin Exp Med 3(1)

      Pages: 27-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Possible modulators of IL-8 and GRO-alpha production by granulosa cells2003

    • Author(s)
      Zeineh K, Kawano Y, Fukuda J, Nasu K, Narahara H, Miyakawa I
    • Journal Title

      Am J Reprod Immunol 50(1)

      Pages: 98-103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Platelet-activating factor inhibits the secretion of platelet-activating factor acetylhydrolase by human decidual macrophages2003

    • Author(s)
      Narahara H, Kawano Y, Nasu K, Yoshimatsu J, Johnston JM, Miyakawa I
    • Journal Title

      J Clin Endocrinol Metab 88(12)

      Pages: 6029-6033

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Effects of colony-stimulating factors on the secretion of platelet-activating factor acetylhydrolase by human decidual macrophages2003

    • Author(s)
      Narahara H, Mine S, Kawano Y, Johnston JM, Miyakawa I.
    • Journal Title

      Am J Obstet Gynecol 188(1)

      Pages: 157-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids2003

    • Author(s)
      Kawasaki F, Kawano Y, Kosay Hasan Z, Narahara H, Miyakawa I.
    • Journal Title

      Clin Exp Med 3(1)

      Pages: 27-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Possible modulators of IL-8 and GRO-alpha production by granulosa cells2003

    • Author(s)
      Zeineh K, Kawano Y, Fukuda J, Nasu K, Narahara H, Miyakawa I.
    • Journal Title

      Am J Reprod Immunol 50(1)

      Pages: 98-103

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Platelet-activating factor inhibits the secretion of platelet-activating factor acetylhydrolase by human decidual macrophages2003

    • Author(s)
      Narahara H, Kawano Y, Nasu K, Yoshimatsu J, Johnston JM, Miyakawa I.
    • Journal Title

      J Clin Endocrinol Metab 88(12)

      Pages: 6029-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The effect of epidermal growth factor on vascular endothelial growth factor secretion by endometrial stromal cells2002

    • Author(s)
      Kawano Y, Nakamura S, Nasu K, Narahara H, Miyakawa I
    • Journal Title

      Clin Exp Med 2(2)

      Pages: 69-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The effect of epidermal growth factor on vascular endothelial growth factor secretion by endometrial stromal cells2002

    • Author(s)
      Kawano Y, Nakamura S, Nasu K, Narahara H, Miyakawa I.
    • Journal Title

      Clin Exp Med 2(2)

      Pages: 69-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Effects of platelet-activating factor on cytokine production by human uterine cervical fibroblasts2001

    • Author(s)
      Sugano T, Narahara H, Nasu K, Arima K, Fujisawa K, Miyakawa I
    • Journal Title

      Mol Hum Reprod 7(5)

      Pages: 475-481

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Effect of endotoxin-induced reactive oxygen species on sperm motility.2001

    • Author(s)
      Urata K, Narahara H, Tanaka Y, Egashira T, Takayama F, Miyakawa I
    • Journal Title

      Fertil Steril 76(1)

      Pages: 163-166

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The effect of dexamethasone on expression of mitogen-induced cyclooxygenase-2 mRNA by amnion-derived(WISH)cells2001

    • Author(s)
      Kawano Y, Nakamura S, Narahara H, Miyakawa I
    • Journal Title

      Eur J Obstet Gynecol Reprod Biol 98(1)

      Pages: 40-45

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Effects of platelet-activating factor on cytokine production by human uterine cervical fibroblasts2001

    • Author(s)
      Sugano T, Narahara H, Nasu K, Arima K, Fujisawa K, Miyakawa I.
    • Journal Title

      Mol Hum Reprod 7(5)

      Pages: 475-81

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Effect of endotoxin-induced reactive oxygen species on sperm motility2001

    • Author(s)
      Urata K, Narahara H, Tanaka Y, Egashira T, Takayama F, Miyakawa I.
    • Journal Title

      Fertil Steril 76(1)

      Pages: 163-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The effect of dexamethasone on expression of mitogen-induced cyclooxygenase-2 mRNA by amnion-derived(WISH)cells2001

    • Author(s)
      Kawano Y, Nakamura S, Narahara H, Miyakawa I.
    • Journal Title

      Eur J Obstet Gynecol Reprod Biol 98(1)

      Pages: 40-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary

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Published: 2001-04-01   Modified: 2016-04-21  

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