A human monoclonal antibody (10-9D) that selectively recognizes human uterine endometrial carcinoma

• Project/Area Number: 13671745
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: TSUKAZAKI Katsumi Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (40118972)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,900,000); Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2001: ¥3,400,000 (Direct Cost: ¥3,400,000)
• Keywords: human monoclonal antibody / cross-bred trans-chemosomic mouse / human endometrial carcinoma / 免疫組織化学染色 / TCマウス / 免疫複合体 / ミサイル療法

Research Abstract

Our newly established human monoclonal antibody termed 10-9D (of IgM subclass) was found to recognized human uterine endometrial cancer carcinoma. Immunohistochemical staining revealed that the reactivity of 10-9D was position in 54.6% of uterine endometrial adenocarcinoma specimens furthermore , the monoclonal antibody does not react with either proliferative or secretory phase normal endometrium. Although slight positivity was observed in the epithelium of the collecting duct in the kidney and gallbladder, no staining was observed in a wide variety of other normal and malignant tissues. Carbohydrate analysis determined that the epitope structure for this antibody is a disulfated mucin-type Olinked carbohydrate structure. These characteristics implicate a possible future diagnostic as well as therapeutic role using the antibody

Research Products

• [Publications] 藤井 多久麿, 塚崎 克己, 野澤 志朗: "ヒトパピローマウィルス感染症の新しい分子標的治療への展望-E2蛋白質をターゲットとして-"産婦人科の実際. 50(1). 105-114 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 久布白 兼行, 岩田 卓, 塚崎 克己: "Ia期FIGO分類とその取扱い"産婦人科治療. 82(2). 169-174 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 久布白 兼行, 舛本 暢生, 岩田 卓, 塚崎 克己, 野澤 志朗: "子宮頸部円錐切除術における妊孕能温存"産科と婦人科. 68(8). 1024-1030 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kyoko Takehara, Kaneyuki Kubushiro, Yuriko Iwamori, Katsumi Tsukazaki, Shiro Nozawa, Masao Iwamori: "Expression of an isoform of the tesis-specific estrogen sulfortransferase in the rine placenta during the late gestational period"Arch. Biochem. Biophys. 394(2). 201-208 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hiroyuki Nakagawa, Kokichi Sugano, Takuma Fujii, Kaneyuki Kubushiro, Katsumi Tsukazaki, Shiro Nozawa: "Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational"Anticancer Research. 22. 1655-1660 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kyoko Takehara, Kaneyuki Kubushiro, Kazushige Kiguchi, Isamu Ishikawa, Katsumi Tsukazaki, Shiro Nozawa, et al.: "Expression of glycolipids bearing Lewis phenotypes in tissues and cultured cells of human gynecological cancers"Jpn. J. Cancer. Res.. 93. 1129-1137 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kyoko,Takehara, Kaneyuki,Kubushiro, Yuriko,Iwamori, Katsumi,Tsukazaki, Shiro,Nozawa, Masao,Iwamori: "Expression of an isoform of the testis-specific estrogen sulfotransferase in the rine placenta during the late gestational period."Arch. Biochem. Biophys. 394(2). 201-208 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hiroyuki,Nakagawa, Kokichi,Sugano, Takuma,Fujii, Kaneyuki,Kubushiro, Katsumi,Tsukazaki, Shiro,Nozawa: "Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism Analysis."Anticancer Research. 22. 1655-1660 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kyoko,Takehara, Kaneyuki,Kubushiro, Isamu,Ishikawa, Katsumi,Tsukazaki, Shiro,Nozawa, Masao,Iwamori: "Expression of glycolipids bearing Lewis phenotypes in tissues and cultured cells of human gynecological cancers."Jpn. J. Cancer Res.. 93. 1129-1137 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hiroyuki Nakagawa, Kokichi Sugano, Takuma Fujii, Katsumi Tsukazaki: "Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism Analysis"Anticancer Research. 22. 1655-1660 (2002)
• [Publications] Kyoko Takehara, Kaneyuki Kubushiro, Katsumi Tsukazaki, Shiro Nozawa: "Expression of glycolipids bearing Lewis phenotypes in tissues and cultured cells of human gynecological cancer"Jpn. J. Cancer Res.. 93. 1129-1137 (2002)
• [Publications] 福地 剛, 久布白 兼行, 塚崎 克己, 野澤 志朗: "加齢と妊娠合併症-子宮頸癌合併妊娠-"産婦人科の実際. 50(12). 1977-1980 (2002)
• [Publications] 進 信幸, 青木 大輔, 塚崎 克己, 野澤 志朗: "病気の形態学"学際企画. 265 (2002)
• [Publications] Yoshibumi Kaneta, Katsumi Tukazaki, Kaneyuki Kubushiro, et.al.: "Selective cytotoxicity of adriamycin immunoconjugate of monoclonal antibody MSN-1 to endometrial adenocarcinoma in vitro and in vivo"Oncology Reports. 7. 1099-1106 (2000)
• [Publications] Kaneyuki Kubushiro, Yuichi Sakuma, Ktsumi Tsukazaki, et.al.: "Biological characteristics of human uterine endometrial cancer variant cells selected for blood group H type 1 antigen"Adhesion to vascular endothelial cells. Acta Histochem Cytochem.. 33(3). 209-213 (2000)
• [Publications] Takuya Irie, Kaneyuki Kubushiro, Katsumi Tsukazaki, et.al.: "Inhibision of attchment and chemotactic invasion of uterine endometrial cancer cells by a new vinca alkaloid,conophylline"Anticancer Res.. 19(4B). 3061-3066 (1999)
• [Publications] Koji Kobiki, Katsumi Tsukazaki, Shiro Nozawa, et.al.: "Detection of endometrial cancer by flow cytometry using two monoclonal antibodies"Cytometry. 36. 150-156 (1999)
• [Publications] Kaneyuki Kubushiro, Jun Ma, Katsumi Tsukazaki, et.al.: "Changes of β-1-4-Galactosyltransferase with the development of endometrial cancer"Gynecol.Obstet.Invest. 48(3). 211-14 (1999)
• [Publications] Yoshibumi kaneta, Katsumi Tsukazaki, Shiro Nozawa, et.al.: "Effect of Gelonin immunoconjugate with monoclonal antibody MSN-1 to endometrial adenocarcinoma on antigen-producing tumor cells in vitro"Jpn.J.Cancer Res.. 89. 583-588 (1998)