|Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
The sensitivity of chemotherapeutic drugs on clear cell carcinoma cells of the ovary (HAC-2, KK-92, KOC-5C, RMG-1, and HCH-1) was evaluated by in vitro colorimetric test, and compared with serous adenocarcinoma cells of the ovary (SHIN-3, SKOV-3, A2780, KF-1, and KOC-2S). Clear cell carcinoma cells showed decrease in sensitivity against docetaxel (P<0.0001), paclitaxel, etoposide, mitomycin, gemcitabine, and cysplatin, compared with serous adenocarcinoma cells. Clear cell carcinoma cells contained 2.1-fold glycogen in the cytoplasm (148.6μg/mg protein vs. 71.1μg/mg protein), and 1.4-fold longer doubling time (40.3hr vs. 28.7hr), compared with serous adenocarcinoma cells ; glycogen content and sensitivity of chemotherapeutic drugs did not show direct relations. In RT-PCR, all clear cell carcinoma cell lines expressed glycogen phosphorylase, glycogen synthetase, and glycogen-6-phosphatase. In glucose deficient condition, doubling time on clear cell carcinoma cells and serous adenocarcinoma cells were elongated for 1.4- and 2.0-times, respectively ; clear cell carcinoma cells were more tolerant in hypoglycemic condition. These results indicate that the poor prognosis in clear cell carcinoma cells is not directly related to resistance to chemotherapeutic agents but seems to be due to the characteristic of cell environment rich in glycogen content.