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Neuroprotective Effects of some drugs on adult rat brain following central auditory tract axotomy

Research Project

Project/Area Number13671774
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKanazawa University

Principal Investigator

ITO Makoto  Kanazawa University, Graduate School of Medical Science, Assistant, 医学系研究科, 助手 (50283106)

Project Period (FY) 2001 – 2004
Project Status Completed(Fiscal Year 2004)
Budget Amount *help
¥3,900,000 (Direct Cost : ¥3,900,000)
Fiscal Year 2004 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 2003 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 2002 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 2001 : ¥1,800,000 (Direct Cost : ¥1,800,000)
KeywordsCommissure of Probst / Facial nerve axotomy / erythropoietin / Toki-shakuyaku-San / G-CSF / Free radical scavenger / ビタミンE / エリスロポエイン / TJ-23 / 当帰芍薬散(TJ-23) / プロブスト交連 / 外側毛帯背側核 / 中枢性聴覚障害 / 神経再生・変性
Research Abstract

oxidative stress and massive production of oxygen-derived free radicals such as nitric oxide(NO) might be one of the most important mechanisms of neuronal cell death following central and/or peripheral nerve injury. This study was aimed to investigate whether recombinant human erythropoietin(rhEPO) and granulocyte colony stimulating factor(G-CSF) exert their neuroprotective effect on the lesioned facial nucleus(FMN) and the dorsal nucleus of lateral lemniscus(DNLL) after nerve injury in the brainstem.
1.After the facial nerve axotomy in the brainstem according to stereotaxic coordinates, the survival ratio of motoneurons in the FMN was 25.0±2.4% on day 14 and 2.8±1.4% on day 28 compared to the contralateral intact side. The survival ratio of motoneurons in the FMN with the rhEPO administration (5000 U/kg daily) was 42.6±6.7% on day 14 and 8.2±0.5% on day 28.
2.The commissure of Probst was transected through its midline decussation with micro knife inserted into the brain according to stereotaxic coordinates. Most of the contralaterally projecting neurons of the DNLL underwent retrograde degeneration on day 14. The survival ratio of the DLL nuerons with the G-CSF administration (50ug/kg daily) was 50% on day 14.
These findings suggest that rhEPO and G-CSF have neuroprotective property to the retrograde degeneration of the FMN and the DNLL after axotomy in the brainstem.

Report

(5results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report

Research Products

(7results)

All 2005 2004 2002 2001

All Journal Article

  • [Journal Article] Neuroprotective effects of recombinant human erythropoietin on adult rat facal motoneuronal death after axotomy in brainstem2005

    • Author(s)
      Ryo Higashi
    • Journal Title

      Neurotrauma Res Vol.16

      Pages : 6-9

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Successful Treatment of Hemifacial Spasm With Selective Facial Nerve Block Using Doxorubicin(Adriamycin) Under Local Anesthesia2004

    • Author(s)
      Makoto Ito
    • Journal Title

      Acta Otolaryngol Vol.124

      Pages : 217-220

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neuroprotective effects of recombinant human erythropoietin on adult rat facial nucleus following nerve transection in the brainstem2004

    • Author(s)
      Ryo Higashi
    • Journal Title

      J.Juzen Med Soc Vol.113

      Pages : 34-44

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ラット脳幹内軸索損傷により惹起される顔面神経の逆行性変性に対するエリスロポエチンの神経保護効果の検討2004

    • Author(s)
      東 良
    • Journal Title

      金沢十全医学会雑誌 113(1)

      Pages : 34-44

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Successful Treatment of Hemifacial Spasm With Selective Facial Nerve Block Usine Doxorubicin(Adriamycin) under Local Anesthesia2004

    • Author(s)
      Makoto Ito
    • Journal Title

      Acta Otolaryngol 124

      Pages : 217-220

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Degeneration and Preservation of the Rat Facial Nucleus Following Axotomy in the Brainstem2002

    • Author(s)
      Tsuyoshi Mashitani
    • Journal Title

      J.Juzen Med Soc Vol.111

      Pages : 348-361

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Degeneration of facial nucleus following the nerve transection in brainstem2001

    • Author(s)
      Tsuyoshi Mashitani
    • Journal Title

      Neurotrauma Res Vol.13

      Pages : 1-3

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary

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Published : 2001-04-01   Modified : 2016-04-21  

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