• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of immuno injury on inner ear autoimmune disease

Research Project

Project/Area Number 13671806
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionNippon Medical School

Principal Investigator

TOMIYAMA Shunichi  Nippon medical School, Otorhinolaryngology, Professor, 医学部, 教授 (00094665)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsinner ear / autoimmune / experimental model / nitric oxide / apotosis / antibody / antigen / whole gel eluter / 内耳自己免疫 / western blotting / whole gel eluter / 免疫組織化学 / 血管条血管 / 自己免疫 / iNOS / マウス / 内耳免疫 / リンパ球 / ゲル分離
Research Abstract

Expression of iNOS was seen in several sites of the cochlea and vestibule one day following secondary immune reaction in the endolymphatic sac. Furthermore, apoptotic degeneration was seen in the spiral ligament and stria vascularis by immunohistochemical expression of ss-DNA, CAD and CPP32. Following recurrent sensitization with crude inner ear antigens of bovine, mouse produced antibodies that reacted several proteins of bovine inner ear as well as mouse kidney, brain, lung and liver. In order to investigate antigenicity of purified inner ear fractioned proteins, crude inner ear antigens were separated into 14 fractions eluted on Mini Whole Gel Eluter. Single sensitization with 55-65 kDa proteins fraction induced the highest number of inner ear infiltrating cells among the fractions. Large volume of fractioned inner ear proteins was obtained by Whole Gel Eluter. Recurrent sensitization off 54〜62kDa inner ear proteins fraction produced antibody that showed single bands with 60-70kDa of bovine inner ear proteins, but not reacted to proteins of mouse those organs. IgG and C3 complement localized in the vessels of the stria vascularis. These results suggest that autoimmune inner ear disease is caused by inner ear specific antigens. Hearing kinetics as well as pathological study is further necessary to investigate specific pathogenic inner ear antigen. Furthermore, proteome analysis of the candidates of inner ear specific pathogenic protein will be essential to identify protein genesis.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Tomiyama S: "Th1;Mediator lymphocytes of experimental autoimmune labyrinthitis"Acta Otolaryngol(Stockh). 121. 673-678 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe K: "Expression of nitric oxide synthase in the cochlea followingImmune response in the endolymphatic sac of guinea pigs."ORL. 63. 155-159 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe K: "Apotosis in the hydropic cochlea of guinea pigs following immune reaction of endolymphatic sac immunohistochemical analysis."Eur Arch Otorhinolaryngol. 258. 296-299 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe K: "Expression of inducible nitric oxide synthase (iNOS/NOS II) in thehydropic vestibule after injection of keyhole limpet hemocyanin into the endolymphatic sac of guinea pig."J Vestibular Res. 11. 67-71 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomiyama S: "Experimental autoimmune labyrinthitis ; Assessment of molecular size of autoantigens in the fractions of innerear proteins eluted on the mini whole gel eluter"Acta Otolaryngol(Stockh). 122. 692-697 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe K: "Expreesion of caspase activated deoxyribonuclease (CAD) and caspase 3 (CPP32) in the hydropic cochlea of guinea pigs.second report"Eur Arch Otorhinolaryngol. 259. 257-261 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 富山俊一: "実験的自己免疫性迷路炎モデル-内耳抗体標的組織の免疫組織学化学的検討-"Otol Jpn. 13. 180-184 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 富山俊一: "メニエール病の免疫傷害病因"J Nippon Med Sch. 70. 212-218 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 富山俊一: "実験的自己免疫性迷路炎モデル-内耳粗抗原感作による内耳抗体の臓器特異性-"Otal Jpn. 14(in print). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 富山俊一: "今日の耳鼻咽喉科頭頸外科治療方針 第2版"医学書院. 624 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomiyama S: "Th1;Mediator lymphocytes of Experimental autoimmune labyrinthitis."Acta Otolaryngol (Stockh). 121. 673-678 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe K: "Expression of nitric oxide synthase in the cochlea following immune response in the endolymphatic sac of guinea pigs."ORL. 63. 155-159 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe K: "Apotosis in the hydropic cochlea of guinea pigs following immune reaction of endolymphatic sac immunohistochemical analysis."Eur Arch Otorhinolaryngol. 258. 296-299 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomiyama S: "Experimental autoimmune labyrinthitis ; Assessment of molecular size of autoantigens in the fractions of inner ear proteins eluted on the mini whole gel eluter."Acta Otolaryngol (Stockh). 122. 692-697 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe K: "Expression of inducible nitric oxide synthase (iNOS/NOS□) in the hydropic vestibule after injection of keyhole limpet hemocyanin into the endolymphatic sac of guinea pig."J Vestibular Res. 11. 67-71 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe K: "Expression of caspase activated deoxyribonuclease (CAD) and caspase 3 (CPP32) in the hydropic cochlea of guinea pigs. second report."Eur Arch Otorhinolaryngol. 259. 257-261 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomiyama S: "Experimental autoimmune labyrinthitis-Immunohisotochemical study of inner ear autoantibody-"Otol Jpn. 13. 180-184 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomiyama S: "Experimental autoimmune labyrinthitis-Organ specificity of inner ear autoantibodies following immunization with crude inner ear antigens-"Otol Jpn. 14(in print). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomiyama S: "Immuno-pathogenesis in Meniere's disease"J Nippon Med Sch. 70. 212-218 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 富山俊一: "実験的自己免疫性迷路炎モデル-内耳抗体標的組織の免疫組織化学的検討-"Otol Jpn. 13(3). 180-184 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 富山俊一: "メニエール病の免役傷害病因"J Nippon Med Sch. 70. 212-218 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 富山俊一: "実験的自己免疫性迷路炎-内耳粗抗原感作による内耳抗体の臓器特異性-"Otol Jpn. 14(1)(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 富山俊一: "今日の耳鼻咽喉科頭頚外科治療指針 第2版(森山寛, 岸本誠司, 小林俊光, 川内秀之)編集"医学書院. 624 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Watanabe K: "Expression of inducible nitric oxide synthase (iNOS/NOS II) in the hydropic vestibule after injection of keyhole limpet hemocyanin into the endolymphatic sac of guinea pig"Journal of Vestibular Research. 11. 67-71 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Watanabe K: "Expression of caspase-activated deoxyribonuclease (CAD) and caspase3C (CPP32) in tte hydropic cochlea of guinea pigs-second report"Eur Arch Otorhinolaryngol. 259. 257-261 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomiyama S: "Experimental autoimmune labyrinthitis : Assessment of molecular size of autoantigens in fractions of inner ear proteins eluted on mini whole gel eluter"Acta Otolaryngol. 122. 692-697 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomiyama S: "Th1 ; Mediator lymphocytes of experimental autoimmune labyrinthitis"Acta Otolaryngol(Stockh). 121. 673-8 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watanabe K: "Expression of inducible nitric oxide synthase in the cochlea following immune response in the endolymphatic sac of guinea pigs"ORL. 63. 155-9 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watanabe K: "Apoptosis in the hydropic cochlea of guinea pigs following immune reaction of the endolymphatic sac : immunohistochemical analysis"Eur Arch Otorhinolaryngol. 258. 296-9 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomiyama S: "Experimental autoimmune labyrinthitis ; Assessment of molecular size of autoantigens in the fractions of inner ear proteins eluted on the mini whole gel eluter"Acta Otolaryngol(Stockh). (in print). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 富山俊一: "内耳自己免疫病(AIED). 今日の耳鼻咽喉科・頭頚部外科治療指針"医学書院. (2002)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi