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Mechanisms of mast cell migration into the allergic nasal epithelium.

Research Project

Project/Area Number 13671807
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionNippon Medical School

Principal Investigator

PAWANKAR Ruby  Nippon Medical School, Otolaryngology, Associate Professor, 医学部, 助教授 (00287674)

Co-Investigator(Kenkyū-buntansha) TAKIZAWA Ryuta  Nippon Medical School, Otolaryngology, Assistant, 医学部, 助手 (10271347)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsNasal Allergy / Mast cell / MC(T) and MC(TC) / RANTES / Stem Cell Factor / Mast cells / MC(T) / Allergy / MC(TC) / Nasal allergy / Mast cell / MC(TC), MC(T) / Stem Cell Factor
Research Abstract

Mast cells are increased in the nasal epithelium of patients with allergic rhinitis (AR). Yet, the precise mechanism is unclear. SCF is considered to be an important chemotactic factor for mast cells. Here we examined the levels of SCF, and other chemokines like RANTES, eotaxin, IL-8 in nasal epithelial layer and deep lamina propria. We also examined the levels released by cultured nasal epithelial cells (CNEC) and fibroblasts. We then analyzed the chemotactic activity of each of these chemokines for mast cells.
By ELISA, we examined the levels of RANTES, eotaxin and SCF in homogenized nasal scrapings and LP of AR patients as well as CNEC and fibroblast culture supernatants. We studied the chemotactic activity by chemotaxis assay.
The levels of RANTES, but not Eotaxin and SCF was greater in the epithelium than in the LP. Moreover CNEC produced higher levels of RANTES but fibroblasts produced higher levels of eotaxin, SCF and IL-8. At the 1 hr time point, RANTES induced more profound chemotasis of mast cells as compared to other chemokines and SCF.
These results strongly suggest that RANTES is a critical factor regulating mast cell migration into the allergic nasal epithelium.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All 2002 2001 Other

All Journal Article (7 results) Publications (14 results)

  • [Journal Article] 超高速回転対応エンドミルチャッキング機構の提案2002

    • Author(s)
      清水伸二 ほか3名
    • Journal Title

      2002年度精密工学会春季大会学術講演会講演論文集

      Pages: 10-10

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Epithelial cells as immunoregulators in allergic airway diseases.2002

    • Author(s)
      Pawankar R.
    • Journal Title

      Curr.Opin.Allergy Immunol. 2(1)

      Pages: 1-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] ミーリンチャック高速回転時の把持力低下防止機構の提案2001

    • Author(s)
      清水伸二 ほか2名
    • Journal Title

      日本機会学会第3回生産加工・工作機械部門講演会講演論文集 01-20

      Pages: 73-74

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Mast cells in Rhinitis2001

    • Author(s)
      Pawankar R., Yamagishi S
    • Journal Title

      Elselvier Publications (Amsterdam)

      Pages: 358-364

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Novel concepts in the pathogenesis of nasal polyps

    • Author(s)
      Pawankar R., Yamagishi S
    • Journal Title

      Proceedings IFOS consensus meeting 2001 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Histamine & Thyptare apregate epithelial cell

    • Author(s)
      Pawankar R., Yamagishi S
    • Journal Title

      Int.Arch.Allergy Immunol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] RANTES regulates mast cell migration in allergic

    • Author(s)
      Pawankar R., Yamagishi S
    • Journal Title

      J.Allergy Clin.Immunol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Pawankar R: "Epithelial Cells as immuno regnlelin allergy airway disease"Curr Opin Allergy Immunol. 2(1). 1-5 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Pawankar R: "Mast cells arches lrators of the allergy reaction"Curr Opin Allergy Immunol. 1(1). 3-6 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nomura I, Katsunuma T, Matsumoto K, Iida M, Tomikawa H, Kawahara H, Tomita H, Akasawa A, Pawankar R, Saito H: "Human mast cell progenitors in peripheral blood from atopic subjects with high IgE levels"Clinical Experimental Allergy. 31(9). 1424-1431 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Pawanker R: "RANTES can regulate mast cell migration …"J. Allergy Clinical Immunology. 107. S167 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Pawankar R: "Synergistic Induction of TADe in nasel eperth cells and fibroblems :(Role of Mest cells)"J. Allergy Clinical Immunol. 111(2). S348 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Pawankar R: "Novel Roles of Mast Cells in Modulating IgE-Mediated Allergic Inflammation"International Archives of Allergy and Immunology. 124. 166-168 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Pawanker R: "Histamine Research in the New Millenium"Elsevier Science. 369-374 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ahn K., Pawankar R: "Regulation of chymase production in human mast cell progenitors"J.Allergy Clinical Immunol.. 106(2). 321-328 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Pawankar R, Yamagishi S: "Novel concepts in the patrogeuesn of nasal polyp"Proceedings of IFOS Consensus Meeting 2001. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Pawankar R, Saito H.: "Histamine & trypsin apregnte epitheliah cell function"Int.Arch.Allergy Immunol. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Pawankar R, Yamagishi S: "Role of scf in regulating mast cell pleuro type"J Allergy Clin.Immunol.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Pawankar R, Yamagishi S.: "RANTES reguletis mast cell migration in allergic …"J.J Allergy Clin.Immunol.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Pawankar R: "Epithelial cells as immunoregulators in allergic airway diseases"Curr.Opin.Allergy Immunol. 2(1). 1-5 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Pawankar R., Yamagishi S: "Mast cells in phinites"Elseivier publicenous (Amsterdam). 358-364 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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