Functional Properties and Regulation of Retinal Pigment Epithelial
| Project/Area Number |
13671816
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Tohoku University |
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Principal Investigator |
SHIMURA Masahiko Tohoku University Hospital, Lecturer, 医学部附属病院, 講師 (20302135)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Katsuhiro Tohoku University Hospital, Associate Professor, 医学部附属病院, 助教授 (20200610)
TOMITA Hiroshi Tohoku University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (40302088)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Retinal pigment epithelium / inward rectifier potassium (Kir) channel / Patch clamp recordings / Ion channel / permeability / Iris pigment epithelium / 毛様体無色素上皮細胞 / Kir7.1チャネル / 硝子体手術 / 浮遊細胞 |
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| Research Abstract |
In the clinical treatment for retinal degenerative disease, iris pigment epithelial cells (IPE) are possible substitutes for retinal pigment epithelial cells (RPE) for transplantation into the subretinal space. It was known that inward rectifier potassium (Kir) channel subunit Kir7.I is functionally expressed in the RPE, plays a central role for maintaining subretinal environment, and has some unique properties which differ from other retinal cells. In the present study, the expression and functional properties of the porcine IPE were investigated, and compared with those in the RPE.
IPE and RPE were acutely dissociated from porcine eyes. Functional properties of Kir channels were characterized using, whole cell patch clamp recording techniques To confirm the unique Kir7.1 electrophysiological properties, permeability (P^<RB>/P^K) and inward slope conductance (g^<Rb>/g^k sequence for monovalent K^+ and Rb^+ were determined. Expression of Kir7.1 mRNA in both cells was detected by reverse
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transcription-polymerase chain reaction (RT-PCR). Whole cell current in the IPE exhibited a mild inward K^+ rectification, and showed little dependence on [K^+]_o. Unusual high (7.04 ± 1.7) Rb^+ t K^+ inward conductance ratio indicated that Kir7.1 subunit was expressed in the IPE as the same as RPE cells. Also, Kir7.1 mRNA was detected in both porcine IPE and RPE by RT-PCR. However, functional expression of Kir conductance in IPE cells (21.7 S/F) was much smaller than that in RPE cells (205.6 S/F). Kir7.1 subunit was predominantly expressed In the acutely dissociated porcine IPE and its functional properties are similar to those in the RPE, however the current density seems too small to fulfill the task of the Kir function of RPE. These results suggested that IPE may have some similar functions of the RPE, and thus supported the idea that IPE cells could be transplanted into the subretinal space to substitute for RPE cells. However before putting the IPE transplantation to practical use, i.e., the treatment for retinal degenerative diseases such as age-related macular degeneration, the amplification of Kir function in the IPE should be considered. Less
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Report
(3 results)
Research Products
(8 results)
