| Project/Area Number |
13671836
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
IKUNO Yasushi (2002-2003) Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (50294096)
日下 俊次 (2001) 大阪大学, 医学系研究科, 講師 (60260387)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSHITA Kenji Osaka University Graduate School of Medicine, Medical Staff, 医学部附属病院, 医員(臨床研究)
OHJI Masahito Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (90252650)
生野 恭司 大阪大学, 医学系研究科, 助手 (50294096)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Retinal pigment epithelium / Kir7.1 / K+ channel / Kir4.1 / Anchoring protein / Patch cramp method / Na+K+pump / Na+-K+チャンネル / K+チャンネル / 内向き整流性K+チャネル / バッチランプ法 / K+チャネル |
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| Research Abstract |
We investigated if Kir7.1 potassium channel in RPE cells. We first performed RT-PCR method to detect mRNA of Kir7.1, and found its expression in cultured RPE cells. We immunized rabbits and prepared polyclonal anti-Kir7.1 antibody. Using this antibody, we detected protein expression of Kir7.1 by western-blotting. We then investigated the localization of Kir7.1 in RPE cells. Immunohistochemistiry was performed, and we found Kir7.1 was localized at the foot of microvilli of the apical base.
We then investigated the localization of other Kir family including Kir4.1 and Kir5.1 in the retina using immunohistochemistry. Kir5.1 was localized in the Muller cells and a part of neural cells of the retina. Its localization in Muller cells was diffuse in the cell body. While, Kir4.1 was localized at the end foot or around the vessel of Muller cells. Immunoprecipitation of Kir4.1 presented Kir5.1, indicating that Kir4.1 and Kir5.1 forms heterogenic tetramer at the cell body and the distal side of Muller cells.
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