| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
To elucidate the role of extracelluar matrix components such as aggrecan, fibronectin and laminin in the extraocular muscle of patients with strabismus, this study underwent. Resected tissues of the medial rectos muscle of 47 patients with intermittent exotropia obtained during strabismus surgery, were frozen under liquid nitrogen and pulverized by a Freezer/Mill to solubilize the tissue for enzyme immunoassay. Aggrecan was measured with Human aggrecan Kit, and for fibronectin and laminin measurements, competitive biding assay was applied by using Quanti Matrix Human Fibronectin Elisa Kit and Quanti Matrix Human Laminin Elisa. The amounts in unit length (mm) of aggrecan, fibronectin, and laminin in the resected tissue were used for statistical analysis to find the relationship with clinical data of patients, such as age, exodeviation, refractive error, pre and postoperative eye position, binocular function, type of intermittent exotropia, family history, abnormalities in pregnancy and delivery. The amount of aggrecan decreased significantly with the advance of age (p<0.0001, Spearman rank correlation test), while the amount of laminin or fibronectin had no correlation with age. Patients with basic type intertmittent exotropia showed larger, although not significantly, amounts of aggrecan than those with convergence insufficiency type (p=0.0538, Mann-Whitney U-test). From these results we concluded that the amounts of aggrecan possibly be related to motor aspecis of intermittent exotropia.
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