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Irsogladine malate : Effect in cataracta diabetica and progress control mechanism from the viewpoint of the gap junction.

Research Project

Project/Area Number 13671843
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionOITA UNIVERSITY

Principal Investigator

FUJIKURA Yoshihisa  OITA UNIVERSITY, ANATOMY, PROFESSOR, 医学部, 教授 (10165368)

Co-Investigator(Kenkyū-buntansha) FUKUMOTO Tetsuo  YAMAGUCHIUNIVERSTIY, ANATOMY, PROFESSOR, 医学部, 教授 (00040171)
KITAMURA Hirokazu  OITA UNIVERSITY, ANATOMY, ASSISTANT, 医学部, 助手 (70115559)
INA Keisuke  OITA UNIVERSITY, ANATOMY, ASSISTANTPROFESSOR, 医学部, 助教授 (20203193)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsRat / Gap junction / Connexin 32 / HAM8 antibody / Intercelluler communication / Streptozotocin / Diabetes mellitus / コネクション32
Research Abstract

1)The establishment of diabetes mellitus model animal.
Of the medicine, Streptozotocin (STZ) which causes the diabetes mellitus to male SD strain rats of the 6〜8 week-old, 6mg/0.1ml/100g, it was injected into vein at the fraction of the body weight The blood glucose level of the animals rose 2 days after the, injection, and the albidus of the crystalline lens was recognized at the 2〜3 month, and the cataract was able to be induced. The macroscope findings were photographed by the newly purchased digital camera, and the results were preserved. The animals were fixed with 3% of paraformaldehyde by low pressure perfusion, and it was made to be post-experimental material that they were frozen or fixed in the alcohol, after retina and crystalline lens were extracted.
2)Experimental groups
Irsogladine malate (IM), 6.7μg/0.3ml/100g body weight of rat, was dissolved in water, and 1 time in every day was administered orally. Diet and water except for the medicine were taken ad libitum and prepared … More following.4 groups as experimental group.
(1)IM was administered every day for I week, then STZ was injected.
(2)STZ was once injected intravenously, then IM was administered every day for 1 week.
(3)STZ was once injected intravenously, then water was administered every day for 1 week.
(4)Physiological saline was once injected intravenously, then water was administered every day for 1 week.
3)Analysis and results
The ocular tissues such as crystalline lens or retina from the experimental or normal rats were made thin sections and they were stained with andi-connexin 32,43 or 50 antibodies. But it was difficult to make sections because of the mixed existance of soft and hard tissues. Now we are trying to make the sentions, therefor we did not get the clear results.
4)Other
The organs which was affected reconstitution of gap junction such as peripheral nerve, liver, kidney, lacrimal gland and so on, were examined. Apart of data was reported in J. Electron Microsc., Prog. Diabetes Research and Med. Electron Microsc. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Keisuke Ina: "Transformation of interstitial fibroblasts and tubulointerstitial fibrosis in diabetic nephropathy."Med.Electron Microsc.. 35. 87-95 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keisuke Ina: "Glomerular podocytte phagocytosis of the diabetic rat."J.Electron Microsc.. 51(4). 275-279 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Noriko Mizutani: "Inflammatory and anti-inflammatory cytokines regulate the recovery from sublethal X irradiation in rat thymus."Radiation Research. 157. 281-289 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuko Tokuda: "MHC class II antigen expression was different from MHC class I antigen expression in irradiated and recovering rat thymus."Acta.Histochemica Cytochemica. 35(2). 101-105 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamani, Arudchelvan: "Spatial relation between major histocompatibility complex-restricted antigen receptor-hearing thymocytes and subtypes of thymic epithelial cells."Journal of Electron Microscopy. 51(3). 173-181 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keisuke Ina: "Progress in Diabetes Research"Nova Science(in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Ina, H.Kitamura, S.Tatsukawa, T.Takayama, Y.Fujikura T.Shimada: "Transformation of interstitial fibroblasts and tubulointerstitial fibrosis in diabetic nephropathy."Med. Electron Microsc.. 35. 87-95 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Ina, H.Kitamura, S.Tatsukawa, T.Takayama, Y.Fujikura: "Glomerular podocytte phagocytosis of the diabetic rat."J. Electron Microsc.. 51(4). 275-279 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Mizutani, Y.Fujikura, YH.Wang, M.Tamechika, N.Tokuda, T.Sawada, T.Fukumoto: "Inflammatory and anti-inflammatory cytokines regulate the recovery from sublethal X irradiation in rat thymus."Radiat. Res.. 157(3). 281-289 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Tokuda, K.Katsube, A.Sakuragi, S.Nagat, D.Harada, Y.Arudchelvan, N.Mizutani, Y-H.Wang, T.Sawada, Y.Fujikura, T.Fukumoto: "MHC class II antigen expression was different from MHC class I Antigen expression in irradiated and recovering rat thymus."Acta Histochem. Cytochem.. 35(2). 101-105 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Arudchelvan, Y.Nishimura, N.Tokuda, T.Sawada, E.Shinozaki, T.Fukumoto: "Differential expression of MI-IC class II antigens and cathepsin L by subtypes of cortical epithelial cells in the rat thymus : an immunoelectron microscopic study"J Electron Microsc (Tokyo). 51. 173-181 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keisuke Ina: "Transformation of interstitial fibroblasts and tubulointerstitial fibrosis in diabetic nephropathy"Med.Electron Microsc.. 35. 87-95 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keisuke Ina: "Glomerular podocytte phagocytosis of the diabetic rat"J.Electron Microsc.. 51(4). 275-279 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Noriko Mizutani: "Inflammatory and anti-inflammatory cytokines regulate the recovery from sublethal X irradiation in rat thymus"Radiation Research. 157. 281-289 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nobuko Tokuda: "MHC class II antigen expression was different from MHC class I antigen expression in irradiated and recoyering rat thymus"Acta.Histochemica Cytochemica. 35(2). 101-105 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamani, Arudchelvan: "Spatial relation between major histocompatibility complex-restricted antigen receptor-bearing thymocytes and subtypes of thymic epithelial cells"Journal of Electron Microscopy. 51(3). 173-181 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 福本哲夫: "胸腺の機能形態学 Functional morphology of the thymus"山口医学. 51(6). 189-192 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keisuke Ina: "Progress in Diabetes Research"Nova Science (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keisuke Ina: "Transformation of interstitial fibroblasts and tubulointerstitial fibrosis in diabetic nephropathy"Medical Electron Microscopy. 35. 87-95 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keisuke Ina: "Glomerular podocytte phagocytosis of the diabetic rat"Journal of Electron Microscopy. 51(4). 275-279 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Noriko Mizutani: "Inflammatory and anti-inflammatory cytokines regulate the recovery from sublethal X irradiation in rat thymus"Radiation Research. 157. 281-289 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nobuko Tokuda: "MHC class II antigen expression was different from MHC class I antigen expression in irradiated and recovering rat thymus"Acta. Histochemica Cytochemica. 35(2). 101-105 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamani, Arudchelvan: "Spatial relation between major histocompatibility complex-restricted antigen receptor-bearing thymocytes and subtypes of thymic epithelial cells"Journal of Electron Microscopy. 51(3). 173-181 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 福本哲夫: "胸腺の機能形態学Functional morphology of the thymus"山口医学別冊. 51(6). 189-192 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takayama T.: "Characteristics Distribution of gap junction in rat lacrimal gland in vivo and reconstruction of gap junction in vitro model"J.Electron Microsc.. 51(1)(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Liu Z.Y.: "Analysis of gap junction formation in rat hepatocytes by intravenous injection of an anti-connexin 32 monoclonal antibody (HAM8)"Anat.Rec.. 262. 147-152 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ikebe T.: "The three-dimensional architecture of renal blood vessels in KK mice, with special reference to the smooth muscle cells and pericytes"J.Electron Microsc.. 50(2). 125-132 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Honda K.: "Molecular basis for h ematopoietic / mesenchymal interaction during initiation of Pever's patch organogenesis"J.Exp.Med.. 193. 621-630 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mizutani N.: "Inflammatory and anti-inflammatory cytokines regulate the recovery from sublethal x-ray irradiation in at thymus"Radi.Res.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 島田達生: "心筋におけるgap junction領域の立体構築"心臓. 32(3). 226-232 (2000)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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