Inhabitation of type insulin-like growth factor receptor stimulates apoptosis induced by anticancer drugs in a human neurobustoma cell live without N-mycamplitication

• Project/Area Number: 13671866
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Osaka University
• Principal Investigator: WASA Masafumi Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (10240467)
• Co-Investigator(Kenkyū-buntansha): KUSAFUKA Takeshi Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助手 (70263267) ; 岡田 正 大阪大学, 医学系研究科, 教授 (40028569)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: Neuroblastoma / N-myc / IGF-1 receptor / 癌増殖

Research Abstract

We investigated whether the blockage of IGF-I receptor (IGE-IR) by a monoclonal antibody against IGF-IR (αIR3) may be advantageously combined with conventional cytotoxic drugs for the design of more effective therapeutic regimens. Here, we used the well-characterized neuroblastoma cell line, SK-N-SH, that is known to express low level of N-myc amplification. Our findings indicate that combined treatment using αIR3 with cisplatin and doxorubicin significantly enhance in vitro cytotoxic effects of chemotherapeutic drugs by stimulating apoptotic process. Therapies that target the IGF-IR combined with traditional anticancer drugs should improve the effectiveness of the chemotherapy and probably even allow the usage of considerably lower doses of toxic drugs.

Research Products

• [Publications] Wasa M, Wang HS, Okada A: "Characterization of L-glutamine transport by a human neuroblastoma cell line"Am J Physiol -Cell Physiol. 282(6). 1246-1253 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Wang HS, Wasa M, Okada A: "Amino acid transport in a human neuroblastoma cell line is regulated by the type I insulin-like growth factor receptor"Life Sci. 71(2). 127-137 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Wang HS, WASA M, Okada A: "Amino Acid transport in a human neuroblastoma cell line is regulated by the typeI insulin-like growth factor receptor"Life Sci. 71(2). 127-137 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Wang HS, WASA M, Okada A: "Regulation of glutamine transport by the type I insulin-like growth factor receptor in a human neuroblastoma cell line"Cancer Lett. 168(1). 81-86 (2001)
• [Publications] Wasa M, Wang HS, Okada A: "Characterization of L-glutamine transport by a human neuroblastoma cell line"Am J Physiol-Cell Physiol. 282(6). 1246-1253 (2002)
• [Publications] Wang HS, WASA M, Okada A: "Amino Acid transport in a human neuroblastoma cell line is regulated by the type I insulin-like growth factor receptor"Life Sci. 71(2). 127-137 (2002)
• [Publications] M.Wasa, HS.Wang, A.Okada: "Characterization of L-glutamine transport by a human neuroblastoma cell line"Am. J. Physiol-Cell Physiol. (in press).
• [Publications] HS.Wang, M.Wasa, A.Okada: "Amino Acid Transport in a Human Neuroblastoma Cell Line is regulated by the type I Insulin-like Growth Factor Receptor"Life Science. (in press).