| Project/Area Number |
13671883
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kyorin University |
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Principal Investigator |
TAKAMI Yoshihiro Kyorin University School of Medicine, Department of Plastic Surgery, Associate Professor, 医学部, 助教授 (30201601)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Tissue-engineered skin / Composite skin substitute / acellular allogeneic dermal matrix / Clinical application / Successful transplantation / Tissue Engineering皮膚 / 複合培養皮膚 / 無細胞真皮マトリックス / 混合培養 |
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| Research Abstract |
The transplantable acellular allogeneic dermal matrix (ADM), which is effective as a scaffold of a tissue engineered skin was developed. By basic studies, following findings were obtained.
1 The preparation of ADM without protease treatment is better for cellular attachment.
2 The sterilization of ADM with high concentration of glycerol is acceptable for improving safety of ADM transplantation.
3 A tissue-engineered skin equivalent based on ADM is homologous to the normal human skin.
4 The cultivating methods without BPE and feeder layers have been established for the regenerative skin surgery.
Based on these findings, I have developed a transplantable tissue-engineered skin equivalent composed of autologous cultured keratinocytes and fibroblasts and decellularized allogeneic dermis. In a 3-week process, cultured autologous keratinocytes from burn patients were incubated with and became firmly bound to decellularized allogeneic dermis obtained from cadavers. The tissue-engineered autologous skin equivalents were transplanted in a one-stage procedure to the excised-third degree burn wounds of 3 patients. The graft survival rates in the 3 patients were 93%, 96%, and 100%, without delayed graft loss or graft fragility.
Conclusion : These results suggest that tissue-engineered skin equivalents based on autologous transformation of decellularized allogeneic dermis can be used for permanent repair of full-thickness skin defects.
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