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Development of Tissue Engineered Skin Equivalent Composed of Autologous Cells and Acellular Allogeneic Dermal Matrix.

Research Project

Project/Area Number 13671883
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionKyorin University

Principal Investigator

TAKAMI Yoshihiro  Kyorin University School of Medicine, Department of Plastic Surgery, Associate Professor, 医学部, 助教授 (30201601)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsTissue-engineered skin / Composite skin substitute / acellular allogeneic dermal matrix / Clinical application / Successful transplantation / Tissue Engineering皮膚 / 複合培養皮膚 / 無細胞真皮マトリックス / 混合培養
Research Abstract

The transplantable acellular allogeneic dermal matrix (ADM), which is effective as a scaffold of a tissue engineered skin was developed. By basic studies, following findings were obtained.
1 The preparation of ADM without protease treatment is better for cellular attachment.
2 The sterilization of ADM with high concentration of glycerol is acceptable for improving safety of ADM transplantation.
3 A tissue-engineered skin equivalent based on ADM is homologous to the normal human skin.
4 The cultivating methods without BPE and feeder layers have been established for the regenerative skin surgery.
Based on these findings, I have developed a transplantable tissue-engineered skin equivalent composed of autologous cultured keratinocytes and fibroblasts and decellularized allogeneic dermis. In a 3-week process, cultured autologous keratinocytes from burn patients were incubated with and became firmly bound to decellularized allogeneic dermis obtained from cadavers. The tissue-engineered autologous skin equivalents were transplanted in a one-stage procedure to the excised-third degree burn wounds of 3 patients. The graft survival rates in the 3 patients were 93%, 96%, and 100%, without delayed graft loss or graft fragility.
Conclusion : These results suggest that tissue-engineered skin equivalents based on autologous transformation of decellularized allogeneic dermis can be used for permanent repair of full-thickness skin defects.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] 高見佳宏: "熱傷治療における人工皮膚の現段階"先端医療27、救急医療の最先端. 第12章-5. 311-316 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 郭静平, 高見佳宏: "消毒・滅菌処理による無細胞真皮マトリックスの特性変化について"杏林医学雑誌. 35(1). 10-21 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高見佳宏ほか: "無細胞真皮マトリックスを担体としたTissue Engineering Skin(複合型培養皮膚)の臨床応用"形成外科. 47(7)(in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshihiro Takami et al.: "Temperature modification in maintenance of 3T3 cells for keratinocytes culture."Skin Surgery. 13(2)(in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高見佳宏, 尾郷賢, 山口亮: "創収縮抑制からみた無細胞真皮マトリックスの製作法の検討"第9回ケロイド・肥厚性瘢痕研究会記録集. No9,2003. 95-103 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Dong Jiaoyun, Yoshihiro Takami: "Protective Effect of radical scavenger, MCI-186 on high-glucose Induced endothelial dysfunction."Wound repair and regeneration. 12:Oct(in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshihiro Takami, YaoMin, Zhao Xiao Yu, Guo JinPing, Ken Ogo, Mika Enoki: "Role of fibroblast-contained collagen sponge on cutaneous wound healing"Proceedings of Keloid Research, 2001, Medical Tribune. 125-131 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideharu Tanaka, Yoshihiro Takami, Seiki Sakaki, Takako Wada: "Choice and clinical effects of various skin substitutes."Igakuno Ayumi. 200(3). 237-242 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideharu Tanaka, Yoshihiro Yamaguchi, Takako Wada, Yoshihiro Takami, et al.: "Choice of fluid resuscitation formula on major thermal injury : Clinical effectiveness of vitamin C therapy."Japanese Journal of Plastic and Reconstructive Surgery. 45(8). 721-731 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideharu Tanaka, Yoshihiro Takami, Hideaki Goto, et al.: "Improved Management in Burn Center of Kyorin University Hospital."Hospitals. 6(3). 232-238 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoyuki Ito, Kazuki Nagayama, Naoko Iino, Isamu Saito, Yoshihiro Takami: "Frontal Sinus Repair with Free Autologous Bone Grafts and Fibrin Glue."Surgical Neurology. 60(2). 155-158 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Guo JingPing, Yoshihiro Takami, Ken Ogo: "Biological behavior of fibroblast in a negative atomospheric pressure environment."Japanese Journal of Plastic and Reconstructive Surgery. 46(11). 111-115 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideharu Tanaka, Yoshihiro Takami, Takeo Koizumi, et al.: "Regenarative medicine in burn treatment."Surgery Frontier. 10(2). 40-47 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akira Nomura, Yoshihiro Takami, Tetsuo Sakai, et al.: "Analysis of gene expression levels in fibroblasts obtained from hypertrophic scar by cDNA expression array."Journal of the Kyorin Medical Society. 35(1). 51-60 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Guo JingPing, Yoshihiro Takami: "Characterization of acellular allogeneic dermal matrix treated with various disinfecting and sterilizing methods."Journal of the Kyorin Medical Society. 35(1). 85-96 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshihiro Takami, Shuji Shimazaki, Ryo Yamaguchi, et al.: "Transplantation of tissue engineered skin equivalent composed of autologous cells and acellular allogeneic dermal matrix."Japanese Journal of Plastic and Recostructive Surgery. 47(8)(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshihiro Takami: Burn Care of particular site. 15(6)(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshihiro Takami, Zhao Xiaoyu, Min Yao, Kiyonori Harii: "Temperature modification in maintenance of 3T3 cells for keratinocytes culture."Skin Surgery. 13(2)(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryo Yamaguchi, Yoshihiro Takami, Guo Jingping, et al.: "The development of the acellular dermal matrix (ADM) for tissue engineered skin."Japanese Journal of Burn Injury. 30(3)(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Dong Jiaojun, Yoshihiro Takami, Qing Chun, Hideharu Tanaka, Ryo Yamaguchi, Guo Jingping, Ken Ogo, Lu ShuLiang: "Effect of A Radical Scavenger, MCI 186 on Glucose Induced Cellular Injury of Cultured Human Dermal Microvascular Endothelial Cells."Journal of Shanghai Second Medical University. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takuya Iida, Yoshihiro Takami, Ryo Yamaguchi, Kiyonori Harii: "Cell damage caused by cell-permeable ceramide and the effect of hepatocyte growth factor and fibroblast growth factor on human dermal fibroblast."Journal of Japan Society of Plastic and Reconstructive Surgery. 24(8)(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Dong Jiaoyun, Yoshihiro Takami, Hideharu Tanaka, Ryo Yamaguchi, Guo Jingping, Qing Chun, Lu Shuliang, Syuji Shimazaki, Ken Ogo: "Protective Effect of a radical scavenger, MCI-186 on high-glucose Induced endothelial dysfunction."Wound repair and regeneration. 12(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshihiro Takami, Ken Ogo, Ryo Yamaguchi: "Preparation of acellular dermal matrix resisting to wound contraction."Proceedings of Keloid Research, 2003, Medical Tribune. 95-103 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Emergency and critical care medicine(Japanese). 16(6). 685-687 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高見佳宏: "熱傷治療における人工皮膚の現段階"先端医療27、救急医療の最先端. 第12章-5(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 郭静平, 高見佳宏: "消毒・滅菌処理による無細胞真皮マトリックスの特性変化について"杏林医学雑誌. 35(1). 10-21 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 高見佳宏ほか: "無細胞真皮マトリックスを担体としたTissue Engineering Skin(複合型培養皮膚)の臨床応用"形成外科. 47(7)(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshihiro Takami et al.: "Temperature modification in maintenance of 3T3 cells for keratinocytes culture."Skin Surgery. 13(2)(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] YOSHIHIRO TAKAMI et al.: "Gene expression of fibroblasts derived from hypertrophic scar using DNA chip"Wound Repair and Regeneration. 10.6. A18 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] MIN YAO, YOSHIHIRO TAKAMI et al.: "Identification, isolation and sorting of human keratinocyte stem cells"Wound Repair and Regeneration. 10.2. A63 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 高見佳宏 他: "表皮細胞と線維芽細胞からなる混合培養シートの創傷治癒における効果"熱傷. 28.4. 273 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshihiro Takami: "Primitive neuroectodermal tumor of the plantar skin"Skin Surgery. 10(1). 17 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Min Yao, Yoshihiro Takami: "Effect of cultured dermal substitute composed of collagen sponge seeded with fibroblasts in simultaneous skin graft overlay"J.Kyorin Medical Society. 32(1). 59-69 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高見佳宏: "繊維芽細胞含有コラーゲンスポンジの創傷治癒に対する効果:同時皮膚移植における影響について"ケロイド・肥厚性瘢痕研究会配録集. 17. 125-131 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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