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Overexpression of antimicrobial peptides in cells makes them resistant bacterial infection

Research Project

Project/Area Number 13671907
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

KAKU Toru  Health Sciences University of Hokkaido, School of Dentistry, Professor, 歯学部, 教授 (60133253)

Co-Investigator(Kenkyū-buntansha) ARAKAWA Toshiya  Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor, 歯学部, 講師 (40306254)
NAKASHIMA Keisuke  Health Sciences University of Hokkaido, School of Dentistry, Associate Professor, 歯学部, 助教授 (80227785)
ABIKO Yoshihiro  Health Sciences University of Hokkaido, School of Dentistry, Associate Professor, 歯学部, 助教授 (90260819)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsDefensin / Transfection / Bacterial infection / Periodontitis / Macrophage / βディフェンシン / 細胞死 / アポトーシス / 抗細菌性タンパク
Research Abstract

Antimicrobial peptides such as β defensins, LL-37, hCAP-18 are involved in defensive function of oral epithelium. It is still unclear how strong protective effect of the peptides on the bacterial infection. The present study examined whether overexpression of the peptides in macrophage cell lines, J774.1, made them resistance bacterial infection.
Two vectors showing β defensin-1 (hBD-1) and -2 (hBD-2) expressions were transfected into J774.1 cell lines by lipofection method. Overexpressions of hBD-1 and -2 in J774.1 cell lines were confirmed by RT-PCR and western blot methods. The cell lines hBDs overexpressed (hBD-1/J774.1, hBD-2/J774.1) and non transfected cell line were used. For bacterial infection in vitro, Actinobacillus actinomycetemcomitance (Aa) was used. The bacterial infections were evaluated as cell death of the cell lines by standard quantitative, MTT and DNA fragmentation assays. The ration of cell death in hBD-1/J774.1 or hBD-2/J774.1 is less than that in J777.1. The results indicate that the overexpression of hBDs make them resistant bacterial infection.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Abiko Y, Jinbu Y, Noguchi T, Nishimura M, Kusano K, Amaratunga P, Shibata T, Kaku T: "Upregulation of human beta-defensin 2 peptide expression in oral lichen planus, leukoplakia and candidiasis. An Immunohistochemical study"Pathol.Res.Pract.. 198. 537-542 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ito Y, Abiko Y, Tanaka Y, Rahemtulia F, Kaku T: "mmunohistochemivcal localization of large chondroitin sulfate proteoglycan in odontogenic tumor"Med Electron Microsc. 35. 173-177 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koyama T, Nakade O, Takada Y, Kaku T, et al.: "Melatonin pharmacologic dose increase bone mass by suppressing resorption through down-regulation of the RANKL-mediated osteoclast formation and activation"J Bone Miner Res. 17. 1219-1229 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abiko Y, Nishimura M, Kusano K, Nakashima K, Okumura K, Arakawa T, Takuma T, Mizoguchi I, Kaku T: "Expression of MIP-3α/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma"Archiv Oral Biol. (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abiko, Y, Suraweera, AK, Nishimura, M, Arakawa, T, Takuma, T, Mizoguchi, I, Kaku, T: "Differential expression of human beta-defensin 2 in keratinized and non-keratinized oral epithelial lesions; immunohistochemistry and in situ hybridization"Virchows Arch.. 438. 248-253 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abiko, Y, Nishimura, M, Rahemtulla, F, Mizoguchi, I, Kaku, T: "Immunohistochemical localization of large chondroitin sulphate proteoglycan in porcine gingival epithelia"European Journal of Morphology. 39. 99-104 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Liu, F, Abiko, Y, Nishimura, M, Kusano, K, Shi, S, Kaku, T: "Expression of inflammatory cytokines and beta-defensin 1 mRNAs in porcine epithelial rests of Malassez in vitro"Med Electron Microse.. 34. 174-178 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abiko, Y, Murata, M, Ito, Y, Taira, T, Nishimura, M, Arisue, M, Inoue, T, Shimono, M, Kuboki, Y, Kaku, T: "Immunohistochemical localization of amelogenin in human odontogenic tumors, using a polyclonal antibody against bovine amelogenin"Med Electron Microsc.. 34. 185-189 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hamaya, M, Mizoguchi, M, Sakakura, Y, Yajima, T, Abiko, Y: "Cell death of osteocytes occurs in rat alveolar bone during experimental tooth movement"Calcif Tissue Int.. 31. 117-126 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koyama, Y, Nakade, O, Takada, Y, Kaku, T, Lau, WK-H: "Melatonin at pharmacologic dose increase bone mass by suppressing resorption through down-regulation of the RANKL-mediated osteoclast formation and activation"J Bone Miner Res.. 17. 1219-1229 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abiko, Y, Jinbu, Y, Noguchi, T, Nishimura, M, Kusano, K, Amaratumga, P, Shibata, T, Kaku, T: "Upregulation of human beta-defensin 2 peptide expression in oral lichen planus, leukoplakia and candidiasis"Pathology. 198. 537-542 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abiko, Y, Nishimura, M, Kusano, K, Nakashima, K, Okumura, K, Arakawa, T, Takuma, T, Mizoguchi, I, Kaku, T: "Expression of MIP-3Eo/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma"Archives of Oral Biol.. 48. 171-175 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abiko, Y, Nishimura, M, Kusano, K, Yamazaki, M, Arakawa, T, Takuma, T, Kaku, T: "Upregulated expression of human E* defensin-1 and -3 mRNA differentiation of keratinocyte immortalized cell lines, HaCaT and PHK16-0b"Journal Dermatol Science. 31. 225-228 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abiko Y, Jinbu Y, Noguchi T, Nishimura M, Kusano K, Amaratunga P, Shibata T, Kaku T: "Upregulation of human beta-defensin 2 peptide expression in oral lichen planus, leukoplakia and candidiasis. An Immunohistochemical study"Pathol. Res. Pract.. 198. 537-542 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ito Y, Abiko Y, Tanaka Y, Rahemtulla F, Kaku T: "mmunohistochemivcal localization of large chondroitin sulfate proteoglycan in odontogenic tumor"Med Electron Microsc. 35. 173-177 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kayama T, Nakade O, Takada Y, Kaku T, et al.: "Melatonin pharmacologic dose increase bone mass by suppressing resorption through down-regulation of the RANKL-mediated osteoclast formation and activation"J Bone Miner Res. 17. 1219-1229 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abiko Y, Nishimura M, Kusano K, Nakashima K, Okumura K, Arakawa T, Takuma T, Mizoguchi I, Kaku T: "Expression of MIP-3α/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma"Archiv Oral Biol. 48(2). 171-175 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshihiro Abiko: "Immunohistochemical localization of amelogenin in human odontogenic tumors, using a polyclonal antibody against bovine amelogenin"Med Electron Microsc. 34. 185-189 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fang Liu: "Expression of inflammatory cytokines and beta-defensin 1 mRNAs in porcine epithelial rests of Malassez in vitro"Med Electron Microsc. 34. 174-178 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogasawara, A.: "Fluid shear stress-induced cycloxygenase-2 expression is mediated by C/EBPb, CREB, and AP-1 in osteoblastic MC3T3-E1 cell."J.Biol.chem.. 276. 7045-7048 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nonaka.K.: "Involvement of caspases in apoptotic cell death of murine macrophages infected with Actinobacillus actinomycetemcomitans."J.Periodont, Res.. 36. 40-47 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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