• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Chemical analysis and immunohistology research on non-collagen protein that takes part in the formation of calcospherites

Research Project

Project/Area Number 13671914
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNihon University

Principal Investigator

MISHIMA Hiroyuki  School of Dentistry at Matsudo, Lecturer (Full-Time), 松戸歯学部, 講師 (30112957)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsGlobular calcification / Linear calcification / Calcospherite / Orientation of apatite crystal / Collagen fibers / Mode of calcification / Crown dentin / Root deuntin / プロテオグリカン / 成長線
Research Abstract

On the mammal, the mineralization of mantle dentin is formed with the mineralization of the matrix vesicle. But the mineralization of circumpulpal dentin is formed by the mineralization that relates to collagen fibers. The spherical calcospherites were observed in the predentin of crown dentin. In the apical region of root dentin, the calcospherites were not observed and the calcified collagen fibers were observed. The species specificity was detected in the development and distribution of calcospherites. As compared with in the cementum covered lingual dentin of continuously growing teeth such as rat and rabbit incisors, and in the cementum covered root dentin uncontinuously growing teeth such as humans and bovine incisors, the form and the size of calcospherites were greatly different. In the marsupial opossum dentin, the calcospherites were only observed at the cervical region of crown. In the reptile crocodilian dentin, the size of the calcospherites was smaller than that of mammal … More , and the fiber-like structure was observed on the surface of calcospherites. On the Ca/P ratio of calcospherites in human, bovine and opossum, the significant difference had been found between the crown and the root of dentin. The difference was not found on the Ca/P ratio of calcospherites in the continuously growing teeth such as rat and rabbit, and polyphyodont crocodilian. It is considered that the difference of the mechanisms of teeth formation related to that of calcospherites formation. With the results of the analytical electron microscopy, except the main element of calcium and phosphorus, elements of Mg S, K, and Al were detected in the stage of calcospherite formation. It was clarified that Mg especially had the key role the formation of the calcospherites among small amount elements from the observation of the analysis electron microscope A lot of Mg was detected in the first stage of the formation of calcospherites. Mg may contribute to physicochemical stability of the apatite crystal. It seems that Mg takes part in the nuclear formation on the initial stage of calcospherites formation. Moreover, it is considered that the localization of S means the existence of the proteoglycan that is non-collagen protein in the calcospherites. It is judged that S of the sulfuric acid radical in the proteoglycan was detected. The calcospherites of crocodilian were small and S was distributed from the cervical region to the root region of dentin. It was considered that the proteoglycan controlled the formation of calcospherites because it took part in the formation of collagen fibers and has closer affinity with Ca. When a human molar was stained with the antiheparan sulfate antibody, the immune reaction attitude was different between the crown pulp and the root pulp. It is considered that this opinion is related to the difference in the form and development of calcospherites between the crown and the root dentin. The reaction was seen well in the region with which dentin was being firmed in the predentin of crown. The calcospherites existed in the mineralization front of dentin, and it was suggested that the form of calcospherites was related to the localization of the heparan sulfate. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 大久保厚司: "糖尿病により口腔インプラント長期経過に伴い撤去に至った-症例-全身疾患の発症と増悪を予知するための血液検査の必要性-"日本歯科先端技術研究所学術会誌. 8・2. 64-69 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takashi Ozaki: "Incremental lines in the dentin of Alligator teeth before and after haching"Nihon University. Journal oral Science. 28・2. 143-151 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大久保厚司: "Ti, Ti 合金インプラント埋入側の口腔底に扁平上皮癌を観た一例-インターネットで検索するTi, Ti合金による癌化の可能性-"日本歯科先端技術研究所学術会誌. 8・3. 118-125 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagai, M.: "Invitro study of collagen coating of titanium implants for intial cell attachment"Dental material Journal. 21・3. 250-260 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大久保厚司: "管理された糖尿病患者の上顎インプラントの長期経過観察"日本歯科先端技術研究所学術会誌. 8・4. 154-160 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Soeda: "Functional characterization of calcium channels localized on the growth cones of cultures rat root ganglion cells"Neuroscience Letters. 325 2002. 5-8 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeru Akazawa: "Neanderthal Burials Excavations of the Dederiyeh Cave Afrin, Syria"University of Tokyo Press Production Center. 394 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kakei, M.: "High-resolution electron microscopy of the crystallites of fossil enamels obtained from various geological ages"JOURNAL OF DENTAL RESEARCH. 80・6. 1560-1564 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kozawa, Y.: "Dental formula of elephant by the development of tooth germ"The world of Elephants, Proceeding of the 1^<st> International Congress. 639-642 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hayakawa, T.: "Influence of bovine dentin site on the bond strength of resin cement"Journal of Hard Tissue Biology. 10・2. 96-102 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 田中譲治: "単純な形態の歯の象牙質における石灰化球形成に関する研究"日大口腔科学. 27・1. 43-53 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 三島弘幸: "マイクロフォーカスX線CT装置を用いたヒト小臼歯の三次元再構築像の観察"日大口腔科学. 26・3. 244-248 (2000)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi