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Regulation of Cbfa1 and IHH expression by retinoid signaling during encondral ossification

Research Project

Project/Area Number 13671935
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOsaka University

Principal Investigator

KISHINO Mitsumobu (2002)  Osaka University Graduate School of Dentistry, Research Associate, 大学院・歯学研究科, 助手 (60346161)

岩本 資己 (2001)  大阪大学, 大学院・歯学研究科, 講師 (80203644)

Co-Investigator(Kenkyū-buntansha) HIRAGA Toru  Osaka University Graduate School of Dentistry, Assistant Professor, 大学院・歯学研究科, 講師 (70322170)
岩本 容泰  大阪大学, 大学院・歯学研究科, 講師 (30223431)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsRetinoid / Cbfa1 / Indian Hedgehog / Enchondral Ossification / Chondrocyte / IHH
Research Abstract

The aim of this study is to prove our hypothesis that retinoid signaling plays a central role in regulation of endochondral ossification by control of expression and function of Indian hedgehog (lhh) and Cbfa1 transcriptionactor. We have found that (1) retinoid signal is activated in the penchondrium close to hypertrophic zone, but not * the other parts of cartilage including the penchondrium, (2) Implantation of beads soaked with retinoids in the deleveloping cartilage induces cbfa1 expression, and implantation of beads soaked with antagonist of retinoids inhibits hypertrophy of chondrocytes, (3) stimulation of hypertrophy of chondrocytes induced by retinoid is completely inhibited by overexpression of dominant negative form of Cbfa1 in vitro, but retinoid antagonist does not disturb the stimulatory action of Cbfa1 on hypertrophy of chondrocytes and (4) retinoid up-regulates expression of lhh in immature chondrocytes while it down-regulates expression of lhh in mature cells. It is suggested that retinoid signal stimulates chondrocyte maturation through up-regulation of cbfa1 expression and that retinoid signal down-regulates lhh expression, and thereby diminish lhh-negative control in chondrocyte maturation. Retinoid signalnight be a key in regulation of the balance between suppression and induction of chondrocyte hypertrophy during endochondral ossification.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Ueta C: "Skeletal malformations caused by overexpression of Cbfa1 or its dominant negative form in chondrocytes"J Cell Biol. 153. 87-99 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Enomoto-Iwamoto M: "Participation of Cbfa1 in regulation of chondrocyte differentiation"Osteoarthritis and Cartilage. 9. S76-S84 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwamoto M: "Runx2 expression and action in chondrocytes are regulated by retinoid signaling and parathyroid hormone-related peptide (PTHrP)"Osteoarthritis Cartilage. 11. 6-15 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koyama E: "Development of stratum intermedium and its role as a Sonic hedgehog-signaling structure during odontogenesis"Dev Dyn. 222. 178-191 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwamoto M: "The role of erg (ets related gene) in cartilage development"Osteoarthritis and Cartilage. 9. S41-S47 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Enomoto-Iwamoto M: "The Wnt antagonist Frzb-1 regulates chondrocyte maturation and long bone development during limb skeletogenesis"Dev Biol. 1;251. 142-156 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueta. C.: "Skeletal malformations caused by overexpression of Cbfa-1 or its dominant negetive form in chondrocytes."J Cell Biol. 153. 87-99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Enomoto-Iwamoto, M.: "Participation of cbfa-1 in regulation of chondrocyte differentiation."Osreoarthrifts and Cartilage. 9. S76-S84 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwamoto, M.: "Runx2 expression and action in chondrocytes are regulated by retinoid signaling and paratbyroid hormone-related peptide (PTHrP)."Osteoarthritis Cartilage.. 11(1). 6-15 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koyama, E.: "Development of stratum intermedium and its role as a Sonic hedgehog-signaling structure during odontogenesis."Dev Dyn. 222. 178-191 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwantoto, M.: "The role of erg (ets related gene) in cartilage development."Osteoarthritis and Cartilage. 9. S41-47 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Enomoto-Iwamoto, M.: "The Wnt antagonist Frzb-1 regulates chondrocyte maturation and long bone development during limb skeletogenesis."Dev Biol.. 1;251(1). 142-156 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueta C: "Skeletal malformations caused by overexpression of cbfa1 or its dominant negative form in chondrocytes"J Cell Biol. 153. 87-99 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Enomoto-Iwamoto M: "Participation of cbfa-1 in regulation of chondrocyte maturation"Osteoarthritis and Cartilage. 9. S76-S84 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwamoto M: "Runx2 expression and action in chondrocytes are regulated by retinoid signaling and parathyroid hormone-related peptide (PTHrP)"Osteoarthritis Cartilage. 11. 6-15 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koyama E: "Development of stratum intermedium and its role as a Sonic hedgehog-signaling structure during odontogenesis"Dev Dyn. 222. 178-191 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwamoto M: "The role of ERG (ets related gene) in cartilage development"Osteoarthritis and Cartilage. 9. S41-S47 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Enomoto-Iwamoto M: Dev Biol. 1;251. 142-156 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueta, C. et al.: "Sekeletal malformations Caused by overexpression of Cbfa1 or its doiminant negative form in chondrocytes"J. Cell Biol.. 153. 87-99 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Enomoto-Iwamoto, M. et al.: "Participation of Cbfa1 in regulation of chondrocyte maturation"Osteoarthritis Cartilage. 9. S76-S84 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Iwamoto, M. et al.: "Ther role of ERG (ets related gene) in cartilage development"Osateoarthiritis Cartilage. 9. S41-S47 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 岩本容泰 et al.: "関節発生と関節軟骨分化制御"現代医療. 33. 37-42 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 岩本資己 et al.: "Wnt可溶型受容体Frzb-1による軟骨細胞の分化制御"実験医学. 19. 1210-1214 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 岩本資己: "軟骨発生機序からみた軟骨の再生"骨・関節・靭帯. 14. 801-808 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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