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Feasibility study for an evaluation system of effect and side effect chemotherapy for oral cancer utilizing time PCR

Research Project

Project/Area Number 13672077
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

MORIKAWA Hidehiro  Tohoku uUniversity Granduate School of Dentistry, Asistant Professor, 大学院・歯学研究科, 助手 (60302155)

Co-Investigator(Kenkyū-buntansha) HIROTANI Hiroaki  Tohoku University, Dental Hospital, Asistant Professor, 歯学部附属病院, 助手 (90312595)
CHIBA Masatoshi  Tohoku University Graduate School of Dentistry, Asistant Professor, 大学院・歯学研究科, 助手 (70261526)
MORI Shiro  Yohoku University, Dental Hospital, Lecturer, 歯学部附属病院, 講師 (80230069)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsoral squamous cell carcinoma / cancer chemotherapy / cisplatin / 5-fluorouracil / side effect / apoptosis-related proteins / mRNA / RT-PCR / 骨髄抑制 / アポトーシス関連タンパク
Research Abstract

The aim of this project is establishment of a mere accurate evaluation system of effect and side effect of chemotherapy for oral cancer utilizing real time PCR. For this purpose, we attempted to examine the mRNA expression of apoptosisrelated proteins in peripheral blood cells and tumor cells of patients with oral squamous cell carcinoma obtained by fine needle aspiration during the course of anticancer chemotherapy. However. Since this kind of examination has not been established so started on a feasibility study using culture cells treated with or without anticancer agents. After the treatment wife cisplatin and/or 5-FU, mRNA expression of apoptosis-mediating proteins inducing Fas,Bax, Caspase-2,3,8,9 in the cultured peripheral blood cells was increased in some cases. On the other hand, in the cultured peripheral blood cells after the treatment with cisplatin and/or 5-FU, some cases also showed increased expression of mRNA of apoptosis-suppressing proteins incliding Bcl-2, Bcl-X_1, c-IAP2, XIAP. These findings suggest that peripheral blood cells may be susceptible to anticancer agents during the course of chemotherapy, and the side effect of the anticancer agents can be evaluated be evaluated by examination of mRNA expression of apoptosis-related proteins. At preaent, we anttempt to start on the clinical study using peripheral blood cells of patients with oral cancer in the course of anticancer chemotherapy. In this study, we will examine the relationship between the expression level of mRNA of apoptosis-related proteins and clinical manifestation of the side effects. Since the know-how that is estabished by feasibility study using peripheral blood cells is applicable to the examination using tumor cells obtained by fine needle aspiration, an evaluation system proposed in this project will yield useful information conceming the effect of anticancer agents.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Hitoshi Miyashita, Shiro Mori, (+3) Atsushi Sato, Hidehiro Morikawa, et al.: "Loss of heteroaygosity of Nucliotide Excision Repair Factors in Sporadic Oral Squamous Cell Carcinoma Using Microdissected Tisuuse"Oncology Reports. 8. 1133-1138 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 佐籐 敦, 友寄泰樹, 山崎慎司, 森 士朗, 森川秀広, 山口 泰: "口腔肩平上皮癌におけるcyclin D1, P16, cyclin dependent kinase 4, RB ptotein, Ki67およびα-cateninの発現"東北大学歯学雑誌. 20(1). 17-26 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 森川秀広, 森 士朗, 篠原文明, 他: "口腔扁平上皮癌細胞における癌化学療法によるアポトーシスの誘導"東北大学歯学雑誌. 20(1). 35-40 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 森川秀広, 森 士朗, 佐藤 敦, 松田耕策, 一迫 玲, 他: "上顎歯肉癌患者にみられた頸部リンパ節炎型トキソプラズマ症の1例"日本口腔外科学会雑誌. 47. 420-423 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hitoshi Miyashita, (2+) Atsushi Sato, Hidehiro Morikawa, (5+) Shiro Mori et al.: "Uridine Phosphorelase is a potential prognostic factor in oral squamous cell carcinoma"Cancer. 94. 2959-2966 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hitoshi Miyashita, Yasutaka Nitta, Shiro Mori, (+5) Atsushi Sato, Hidehiro Morikawa, et al.: "Expression of Copper-Transporting P-Type Adenosine Triphosphatase (ATP7B) as a Chemoresistance Marker in Human Oral Squamous Cell Carcinoma Treated with Cisplatin"Oral Oncology. 39(2). 157-162 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hitoshi Miyashita, Shiro Mori, Naoko Tanda, Kentaro Nakayama, Atsuko,Kanzaki, Atushi Sato, Hidehiro Morikawa, Katutoshi Motegi, Yuji Takebayashi, Manabu Fukumoto: "Loss of heterozygosity of Nucleotide Excixion Repair Factors in Sporadic Cral Squamcus Cell Carcinoma Using Microdissected Tisuuse"Oncology Reports. 8. 1133-1138 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Atsushi Sato, Taiki Tomoyose, Shinji Yamazaki, Shiro Mori, Hidehiro Morikawa, Tai Yamaguchi: "Expression of cyclim D1,P16,cyclin dependent Kinase 4, RB protein, Ki67, and α-catenin in cral squamous cell carcincma"Tohoku University Dental Journal. 20(1). 17-26 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hidehiro Morikawa, Shiro Mori, Humiaki Shinohara, Seishi Echigo: "Induction of apoptosis in oral squamous cell carcinoma by cancer chemctherapy"Tohoku University Dental Journal. 20(1). 35-40 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hidehiro Morikawa, Shiro Mori, Atsushi Sato, Kosaku Matsuda, Ryo Ichinohasama, Seishi Echigo: "A case of lymphadenitis type toxoplasmosis in the cervical lymph nodes of a patient with squtamous cell carcinoma of the upper gingiva"Japanese Joumal of Oral and Maxillofacial Surgery. 47(7). 420-423 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hitoshi Miyashita, Yuji Takebayashi, Yasutaka Nitta, Atsushi Sato, Hidehiro Morikawa, Fumihiro Fujimori, Arita Ohashi, Jatnes F. Fliason, Katutoshi Motegi, Manabu Fukumoto, Shiro Mori, Takafumi Uchida: "Uridine Phcsphctelase is a potential prognostic factor in cral Squamcus cell carcincma"Cancer. 94. 2959-2966 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kentaro Nakayama, Atsuko Kanzaki, Nouri Neamati, Kunihiko Terada, Masato Mutoh, Kenji Ogawa, Shiro Mori, Hitoshi Okamura, Kohji Miyazaki, Toshihiro Sugiyama, Manabu Fukumoto, Yuji Takebayshi: "Expression of Copper-Transporting P-type Adencsine Triphosphatase (ATP7B) as a Chemoresistance Marker in Human Ovarian Carcinoma Treated with Cisplatin"Oral Oncology. 39(2). 157-162 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Miyashita, H., Y., Mori, S., (+5)Sato, A., Morikawa, H., Motegi, K., Takebayashi, Y.: "Expression of Copper-Transporting P-type Adenosine Triphoshatase(ATP7B) as a Chemoresistance Marker in Human Oral Squamous cell carcinoma Treated with Cisplatin"Oral Oncology. 39(2). 157-162 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyashita.H, Mori.S, Tanda.N, Nakayama.K, Kanzaki.A, Sato, A, Morikawa.H, et al.: "Loss of heterozygosity of nucleotide excision repair factor in sporadic oral squamous cell carcinoma using microdissected tissue"Oncology Reports. 8. 1133-1138 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyashita.M, Takebayashi.Y, Nitta.Y, Sato.A, Morikawa.H, (+5), Mori.S, Uchida.F: "Uridine phosphorelase is a potential prognostic factor in oral squamous cell carcinoma"Cancer. In press.

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤敦, 友寄泰樹, 山崎慎司, 森士朗, 森川秀広, 山口泰: "口腔扁平上皮癌におけるCyclin D1. p16 cyclin dependent kinase 4. RBprotein, Ki67およびα-cateninの発現"東北大歯誌. 20(1). 17-26 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 森川秀広, 森士朗, 篠原文明, 他: "口腔扁平上皮癌細胞における癌化学療法によるアポトーシスの誘導"東北大歯誌. 20(1). 35-40 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 森川秀広, 森士朗, 佐藤敦, 松田耕策, 一迫玲, 他: "上顎歯肉癌患者にみられた頸部リンパ節炎型トキソプラズマ症の1例"日口外誌. 47. 420-423 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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