| Project/Area Number |
13672108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
ETSUKI Shinno University of the Ryukyus, University of the Ryukyus, Faculty of Medicine, instructor (60325842)
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| Co-Investigator(Kenkyū-buntansha) |
YOKO Aniya University of the Ryukyus, Graduate Schools of Medicine, Professon (90045055)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | temporomandibular joint / synovial fluid / free radicals / sodium hyaluronate / temporomandibular joint disorder |
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| Research Abstract |
The painful, dysfunctional temporomandibular joint (TMJ) is caused by stress-related, prolonged microtrauma. The mechanical stress leads to the accumulation of free radicals in affected articular tissues of susceptible individuals. The main component of synovial fluid (SF) is said to be sodium hyaluronate (SH). Free radical affects SH followed by TMJ injury. We investigate antioxidant action of sodium SH and SF. 【Materials and methods】 SF samples were taken from 7 TMJs of 7 patients who had been diagnosed as nonreducing disk displacement (NDD) and 3 TMJs of asymptomatic volunteers by injection of 1.5 ml
lidocaine into the intra-articular of TMJ. In all patients NDD was confirmed by magnetic resornance imaging. NO content in SF was determined by the NO analyzer. Radical scavenging action of SF, SH and 1% lidocaine were evaluated by ESR spectrometer, using ?OH generated by Fenton reaction (0.01mM FeSO4 and 1mM H202). 【Results】 NO was detected in 2 TMJs of 7 patients, not in 3 TMJs of 3 asymptomatic volunteers. Inhibition rate for? OH of SF from patients, volunteers and 1% lidocaine was 46.7±9.2%, 64.5±2.6% and 41.4±4.1%, respectively. Thus it was observed that the radical scavenging action of SF from patients was lower than that of asymptomatic volunteers, suggesting consumption of antioxidant with radicals in TMJ of patients.
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