The Study of the Apoptosis of Osteocytes

• Project/Area Number: 13672157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 矯正・小児・社会系歯学
• Research Institution: The University of Tokushima
• Principal Investigator: MIKI Yoshiki The University of Tokushima, School of Dentistry, Assistant Professor, 歯学部, 助手 (50294707)
• Co-Investigator(Kenkyū-buntansha): MORIYAMA Keiji The University of Tokushima, School of Dentistry, Professor, 歯学部, 教授 (20262206) ; YOKOZEKI Masahiro The University of Tokushima, School of Dentistry, Associate Professor, 歯学部, 助教授 (10314866) ; HORIUCHI Shinya The University of Tokushima, School of Dentistry, Assistant Professor, 歯学部附属病院, 助手 (70263861)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: osteocytes / apoptosis / hypoxic condition / 低酸素濃度 / 単離骨細胞 / H_2O_2

Research Abstract

With respect to the biology of tooth movement, it is known that the periodontal ligament change into the hyaline degeneration at the pressure side and osteoclasts do the bone resorption at the area adjacent to the hyalinized periodontal ligament. Recently, osteocytes die via a mechanism that is similar to apoptosis at the first stage of the bone remodeling. It is known that the activated oxygen induced in the ischemia-reperfusion causes the apoptosis. When isolated rat osteocytes were cultured with hydrogen peroxide (H_20_2), there was a dose dependent increase in trypan blue, which is the marker of apoptosis, positive cells. Furthermore, we 'also examine the response of osteocytes in the hypoxic condition. When osteocytes were cultured in the hypoxic condition, there was a dose dependent increase of osteocytes apoptosis. Taken together, hydrogen peroxide and hypoxic condition play an important role of osteocytes apoptosis.