Effects of aging on IL-1β and PGE_2 production in the periodontal ligament cells with mechanical stress.
| Project/Area Number |
13672171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Nihon University |
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Principal Investigator |
GOSEKI Takemi Nihon University, School of dentistry at Matsudo, Lecturer (Full-Time), 松戸歯学部, 講師 (20225642)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | aging / Mechanical stress / Periodontal ligament cells / ヒト歯根膜細胞 / メニカルストレス / NF-kB |
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| Research Abstract |
Although the severity of periodontal disease is known to be affected by the age of the host, the pathological role of aging in periodontal disease, especially that attributable to trauma from occlusion, has not been well characterized. Prostaglandin (PG) E_2 and interleukin (IL)-1β are key mediators involved in periodontal diseases, potent stimulators of bone resorption, and are produced by human periodontal ligament (PDL) cells in response to mechanical stress. To investigate age-related changes in the biosynthetic capacity of PGE_2 and IL-1β in PDL cells, we examined the effects of in vivo aging with mechanical tension on PGE_2 and IL-1β expression by rat PDL cells. PDL cells obtained from the incisors of 6-week (young) and 60-week (old) rats were cultured on flexible-bottomed culture plates. The cells were deformed by causing a 9% or 18% increase in surface area at 6 cycles per minute for 1 to 5 days. We found an approximately twofold increase in PGE_2 and IL-1b production by old PDL cells subjected to mechanical tension compared with that by young cells, although the constitutive levels were similar in both. The expression of cyclooxygenase (COX)-2 and IL-1β mRNA was enhanced by mechanical tension as determined by use of reverse transcription-polymerase chain reaction (RT-PCR), whereas COX-1 and IL-1β-converting enzyme mRNA remained unchanged. It is possible that the large amount of PGE\2 and IL-1β produced by PDL cells from an aged host in response to mechanical force may be positively related to the acceleration of alveolar bone resorption.
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Report
(4 results)
Research Products
(6 results)
