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Analysis of bacterial biofilms as a virulence factor for peri-implantitis

Research Project

Project/Area Number 13672184
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka University

Principal Investigator

NOIRI Yuichiro  Osaka University, Graduate School of Dentistry, Research Associate, 大学院・歯学研究科, 助手 (50218286)

Co-Investigator(Kenkyū-buntansha) EBISU Shigeyuki  Osaka University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (50116000)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsperi-implantitis / bacterial biofilm / implant sulcus / immunohistQchemical stain / Porphyromonas gingivalis / periodontopathic bacteria / chlorhexidine gluconate / cloning / Porphyromonas gingivallis / 走査型電子顕微鏡 / 共焦点レーザー顕微鏡 / 蛍光染色
Research Abstract

We investigated morphologically initial biofilm formation in the human implant sulcus using healing abutments. After obtaining informed consent from 7 patients, twenty healing abutments were removed carefully on day 10, 30, 90 and 150 after insertion and treated for a scanning electron microscopy. On day 10, mono-layer biofilm have partially formed on the abutment surfaces. So called early colonizers: cocci and filaments, were observed. After 30 days, biofilms developed, and long and short rods were predominantly observed in human implant sulcus
Next, human periimplantitis associated bacteria have been examined immunohistochemically. Eight implants obtained from 4 voluntary patients were extracted and processed into semithin sections They were stained BrownBrenn modified Gram stain and streptavidinbiotin method using 4 kinds of antibodies with periodontopathic bactria. Gram positive bacteria were shown not only on the implant surface but also at the inner site of abutment screw, Porphyromonas gingivalis and Campylobacter rectus labeled with speciesspecific antibody were not found in the human implant sulcus
By use of the in vitro model with P. gingivalis biofilm, 1) the biological (ATP) activities, 2) the susceptibility to antimicrobial agents, and 3) the essential gene for development of biofilm (the homologue for polyphosphate kinase (ppk)) were examined. The rate of increase of ATP contents in the biofilm was quite low, and 1/3.0x10^5 compared with that of planktonic cells. Chlorhexidine gluconate was effective at reducing the viability of P. gingivalis biofilm, while minocycline hydrochloride and metronidazole did not have a variable effect. Identity of P. gingivalis ppk gene that was carried out cloning to Esherihia coli ppk gene was 35.8%, including \hat ppk from P. gingivalis was highly conserved

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Noiri Y. et al.: "The localization of periodontal disease-associated bacteria in human periodontal pockets"Journal of Dental Research. 80・10. 1930-1934 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 成松雅博: "歯周病原性細菌Porphyromonas gingivalisバイオフィルムの生物学的活性の評価およびバイオフィルム関連遺伝子の検索"Bacterial Adherence & Biofilm. 15. 8-13 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Noiri Y. et al.: "Effects of chlorhexidine, minocycline and metronidazole on Porphyromonas gingivalis strain 381 in bioflim"Journal of Periodontology. 74(印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 偲比須繁之(分担執筆): "バイオフィルム(Bacterial Biofilm):最近話題の用語 -知っておきたい豆知識 -"小児科. 44・4(増刊号)(印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Noiri,Y: "The localization of periodontal disease-as sociated bacteria in human periodontal pockets"J Dent. Res. 80(10). 1930-1934 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Narimatsu,M: "Evaluation of baiological activity of periodontopathic bacterium Porphyromonas ging ivalis biofilm and Characterization of the biofilm-related genes"Bacterial adherence & Biofilm. 15. 8-13 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Noiri,Y: "Effects of chlorhexidine, minocycline and metronidazole on Porphyromonas gingivalis strain 381 in biofilm"J Periodcntal. 74 In press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ebisu,S: "Bacterial biofilm"Jpn J Pediatrics. Special Issue In press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 成松雅博: "歯周病原性細菌Porphyromonas gingivalisバイオフィルムの生物学的活性の評価およびバイオフィルム関連遺伝子の検索"Bacterial Adherence & Biofilm. 15. 8-13 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Noiri Y., et al.: "Participation of bacterial biofilm in refractry and chronic periapical periodontitis"Journal of Endodontics. 27・10. 679-683 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 成松雅博: "Porphyromonas gingivalisのputative glycosyltransferase geneのクローニングおよびバイオフィルム形成における役割解析"Bacterial Adherence & Biofilm. 16(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Noiri Y., et al.: "Effects of chiorhexidine, minocycline and metronidazole on Porphyromonas gingivalis strain 381 in biofilm"Journal of Periodontology. 74(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 恵比須繁之(分担執筆): "バイオフィルム(Bacterial Biofilm):最近話題の用語-知っておきたい豆知識-"小児科. 増刊号特集(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 恵比須繁之: "バイオフィルム感染症としての歯周病の特徴"炎症・再生. 21. 141-147 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Noiri Y. et al.: "The localization of periodontal disease-associated bacteria in human periodontal pockets"Journal of Dental Research. 80. 1930-1934 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Noiri Y. et al.: "Participation of bacterial biofilm in refractory and chronic periapical periodontitis"Journal of Endodontics. 28(印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2018-12-05  

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