| Project/Area Number |
13672189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
YONEDA Masahiro Faculty of Dental Science, Research Associate, 大学院・歯学研究院, 助手 (10253460)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Koji Nagasaki University Professor, 歯学部, 教授 (80150473)
HIROFUJI Takao Fukuoka Dental College Professor, 口腔歯学部門, 教授 (10189897)
MAEDA Katsumasa Faculty of Dental Science, Professor, 大学院・歯学研究院, 教授 (00117243)
HAMACHI Takafumi Dental Hospital Associate Professor, 歯学部附属病院, 講師 (80198811)
ANAN Hisashi Dental Hospital Associate Professor, 歯学部附属病院, 講師 (80158732)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Porphyromonas gingivalis / Bacteroides forsythsus / mixed infection / murine abscess model / gingipain / T cell / cytokine / chemokine / システインプロテアーゼ |
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| Research Abstract |
We examined the effect of mixed infection of Porphyromonas gingivalis and Bacteroides forsythus on abscess formation using murine abscess model. When these two bacteria were injected together, they exhibited synergistic effect comparing to the mono-infection by each bacterium. The mutant strains of P. Gingivalis devoid of gingipains exhibited reduced abscess forming abilities comparing to the wild strain. When these mutant strains were used instead of the wild strain in the mixed infection experiments, they only showed additive effects. These results indicate that periodontopathogens exhibit synergism in the periodontal environment and gingipains are playing an important role in the mixed infection.
We also examined the effect of P. gingivalis on T cells. The culture supernatant of this bacterium was found to have CD4-cleaving activity. And it was revealed that gingipains were playing an important role in this activity.
We also revealed that cytokines, chemokines, and receptors for these are playing an important role in the marginal and periapical periodontitis. They were found to be associated with the progression of periodontitis in Papillon-Leffevre Syndrome, too. We also reported the function of cytokines in the rat experimental periapical periodontitis.
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