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Synthesis of Peptide Mimetics of RNA that Regulate the Activity of Teromerase

Research Project

Project/Area Number 13672209
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical pharmacy
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

KAJIMOTO Tetsuya  Tokyo Univ. of Agr. & Tech., Department of Biotechnology, Associated Professor, 工学部, 助教授 (80185777)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsteromerase / primer RNA / peptidic RNA / L-threonine aldolase / β-hydroxy-α-L-amino acid / uracil / adenine / cytosine / 逆転写酵素 / RNA / ペブチド性RNA / テロメラーゼ阻害剤 / アデニン(A) / ウラシル(U) / DCC / HOBt / NMM法
Research Abstract

Teromerases, a family of reverse transcription enzymes, show potent activity in tumor and generative cells, and utilizes RNA as a mold (referred to teromerase RNA) on elongating the repeating DNA sequences, which feature the teromea structure, at the 3'-terminus of DNA. After elongating the several times of the repeating DNA sequences, the complement RNA is synthesized and works as a primer and duplication of DNA proceeds. As a result, the duplicated DNA is no shorter than the original DNA, and both the primer RNA and the teromerase RNA should have the same base sequences "UAACCCUAA". Therefore, mimetics of primer RNA (equivalent to teromerase RNA) could be an excellent candidate of novel medicines that regulates teromerase activities to ssuppress the endless growth of tumor cells, of which teromerase activity is high.
Now, we designed a peptide mimetic of the primer RNA (peptidic RNA) as an inhibitor of teromerases, and embarked on the synthetic study. At first, the acetaldehyde derivatives having uracil (U), adenine (A), or cytosine (C) residue at α-carbon were respectively treated with glycine in the presence of L-threonine aldolase to afford β-hydroxy-α-L-ammo acids carrying the RNA bases at γcarbon (The amino acids are abbreviated as γU-Thr, γA-Thr, γC-Thr). The amino acids were derived to benzyl esters, and followed by the reaction with Boc-Gly-Osu to give dipeptides, Boc-Gly-γUThr(Obn), Boc-Gly-γAThr(Obn), and Boc-Gly-γCThr(Obn). The conventional peptide synthesis using DCC/HOBt/NMM, deprotection of Boc with HC1, and cleavage of benzyl ester by alkaline hydrolysis or hydrogenation were repeated, and this synthetic procedure linking the dipeptides correctly gave two identified hexa-peptides having UAA or CCC codes on the residues. Final stage for the synthesis of the target peptidic RNA coding UAACCCUAA bases is in progress and will be accomplished in the near future.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] T.Miura, T.Kajimoto: "Application of L-Threonine Aldolase-Catalyzed Reaction to the Preparation of Protected 3R,5R-Dihydroxy-L-homoproline as a Mimetic of Idulonic Acid"Chirality. 13. 577-580 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Ikeda, H.Miyashita, T.Kajimoto, T.Nohara: "Synthesis of neosaponins having an α-L-rhamnopyranosyl-(1-4)-[α-L-rhamnopyranosyl-(1-2)]-D-glucopyranosyl glyco-linkage"Tetrahedron Lett.. 42. 2353-2356 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Demura, M.Noda, T.Kajimoto, T.Uchiyama, et al.: "Structure of sialyl Lewis X mimics studied by two-dimensional NMR"J.Mol.Structure. 602-603. 215-222 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Kajimoto, N.Takanashi, T.Kajimoto, M.Xu, J.Cao, et al.: "Sophoranone, Extracted from a Traditional Chinese Medicine Shan Dou Gen, Induces Apoptosis in Human Leukemia U937 Cells via Formation of Reactive oxygen species and opening of mitochondrial permeability transition pores"Int.J.Cancer. 99. 879-890 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Tanaka, M.Ozawa, T.Miura, T.Inazu, S.Tsuji, T.Kajimoto: "Synthesis of Novel Mimetics of CMP-Sialic Acid as the Inhibitors of Sialyltransferases"Synlett. 9. 1487-1490 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 中村洋編 梶本哲也共著: "試料の前処理ハンドブック"丸善. 1053 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Miura, T. Kajimoto: "Application of L-Threonine Aldolase-Catalyzed Reaction to the Preparation of Protected 3R, 5R-Dihydroxy-L-homo-proline as a Mimetic of Idulonic Acid"Chirality. 13. 577-580 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Ikeda, H. Miyashita, T. Kajimoto, T. Nohara: "Synthesis of neosaponins having an α-L-rhamnosyl-(1-4)-[α-L- rhamnosyl-(1-2)]-D-glucopyranosyl glyco-linkage"Tetrahedron Lett.. 42. 2353-2356 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Demura, M. Noda, T. Kajimoto, T. Uchiyama, K. Umemoto, C.-H. Wong, T. Asakura: "Solution structure of sialyl Lewis X mimics studied by two-dimensional NMR"J. Mol. Structure. 215-222 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Kajimoto, N. Takanashi, T. Kajimoto, M. Xu, J. Cao, Y. Masuda, T. Aiuchi, S. Nakajo, Y. Ida, K. Nakaya: "Sophoranone, Extracted from a Traditional Chinese Medicine Shan Dou Gen, Induces Apoptosis in Human Leukemia H937 Cells via Formation of Reactive Oxygen Species and Opening of Mitochondrial Pearmiability Trarisition Pores"Int. J. Cancer. 99. 879-890 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Tanaka, M. Ozawa, T. Miura, T. Inazu, S. Tsuji, T. Kajimoto: "Synthesis of CMP-sialic Acid Analogues as the Inhibitors of Sialyltransferases"Synlett. 1487-1490 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Tanaka, T.Miura, T.Inazu, S.Tsuji, T.Kajimoto: "Synthesis of Mimetics of CMP-Sialic Acid as the Inhibitors of Sialyltransferases"Synlett. No.9. 1487-1490 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Demura, M.Noda, T.Kajimoto, T.Uchiyama, K.Umemoto, C.-H.Wong, T.Asakura: "Solution structure of sialyl Lewis X mimics studied by two-dimensional NMR"J.Mol.Structure. 602-603. 215-222 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Kajimoto, N.Takanashi, T.Kajimoto, M.Xu, J.Cao, Y.Masuda, T.Aiuchi et al.: "Sophoranone,?extracted from a traditional Chinese medicine Shan Dou Gen, induces apoptosis in?human leukemia U937 cells via formation of reactive oxygen species and opening of?mitochondrial permeability transition pores"Int.J.Cancer. 99. 879-890 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中村洋 編, 梶本哲也 共著: "試料の前処理ハンドブック"丸善. 1053 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T. keda, H.Miyashita, T.Kajimoto, T.Nohara: "Synthesis of neosaponins having an α-L-rhamnosy1-(1, 4)-[α-L-rhamnopyranosy1-(1, 2)]-D-glucopyranosyl glyco-linkage"Tetrahedron Lett.. 42. 2353-2356 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Miura, T.Kajimoto: "Application of L-Threonine Aldolase Catalyzed Reaction to the Preparation of the Protected 3R, 5R-Dihydroxy-L-homo-proline as a Mimetic of Idulonic Acid"Chirality. 13. 577-580 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Demura, M.Noda, T.Kajimoto, T.Uchiyama, K.Umemoto, C. H. Wong, T.Asakura: "Solution structure of sialyl Lewis X mimics studied by two-dimensional NMR"J. Mol. Structure. 602. 215-222 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Kajimoto, N.Takanashi, T.Kajimoto, M.Xu, J.Kao, Y. Masuda, T. Aiuchi, et al.: "Sophoranone, extracted from a traditional Chinese Medicine, Shan Dou Gen, induced apoptosis in human leukemia U937 cells via formation of reactive oxygen species and opening of ---"Int. J. Cancer. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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