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Imprinted Potymer based Sensor Array Plate

Research Project

Project/Area Number 13672260
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionKobe University (2002)
Hiroshima City University (2001)

Principal Investigator

TAKEUCHI Toshifumi  Kobe University Graduate School of Science and Technology Professor, 自然科学研究科, 教授 (70179612)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsmolecular imprinting / molecular recognition / metallo-porphyrins / sensing / 鉄ポルフィリン
Research Abstract

1. A diastereoselective molecubrly imprinted polymer (MIP) for (-) cinchonidine, PPM (CD), was prepared by the combined use of methacrylic acid and vinyl-substituted metallo-porphyrin as functional monomers. Compared to MIPs using only methaciylic acid or metallo-porphyrin as a functional monomer, PM (CD) and PP (CD), respectively, PPM (CD) showed higher binding ability for (-) -cinchonidine in chromatographic tests using the MIPs-packed columns. The MIPs containing a metallo-porphyrin in the binding sites, PPM (CD) and PP (CD), showed spectroscopic change according to the binding of (-) -cinchonidine and it was significant in the low concentration range, suggesting that the high affinity binding sites contain the porphyrin residue. The correlation of the spectroscopic change against log of (-) -cinchonidine concentrations showed a linear relationship. These results revealed that the MIP having highly specific binding sites was assembled by the functional monomers, vinyl-substituted metallo-porphyrin and methacrylic acid, and they cooperatively worked to yield the specific binding. In addition, the metallo-porphyrin-based MIPs appeared to act as a sensor selectively responded by binding events of the template molecule.
2. A molecularly imprinted polymer library was prepared on a 96-well microtiter plate using a liquid-handing equipment that was programmed to prepare various breeds of cinchonidine-imprinted polymers. The resultant polymers immobilized on the bottom surface of the wells were screened for the binding capacity by fluorescence measurement using a microplate reader. Comparison with a conventional assessment by FIA demonstrated the reliability and the high-throughput performance of the microtiter plate-based combinatorial molecular imprinting.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Takeuchi, T.: "Molecularly Imprinted Polymers with Metalloporphyrin-Based Molecular Recognition Sites Coassembled with Methacrylic acid"Anal.Chem.. 73. 3869-3874 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeuchi, T.: "Molecularly Imprinted Polymer Library on a Microtiter Plate : High-throughput Synthesis and Assessment of Cinchona Alkaloid-imprinted Polymers"Instrument Sci.Technol.. 29. 1-9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kugimiya, A: "Synthesis of 5-Fluorouracil-Imprinted Polymers with Multiple Hydrogen Bonding Interaction"Analyst. 126. 772-774 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kugimiya, A.: "Preparation of Sterol-Imprinted Polymers with the Use of 2-(Methacryloyloxy)ethyl Phosphate"J.Chromatogr. A. 938. 131-135 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kugimiya, A: "Molecular Recognition in Indoleacetic Acid-Imprinted Polymers Effect of 2-Hydroxyrthyl Methacrylate Contents"Anal.Bioanal.Chem. 2002. 372. 305-307 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeuchi,T., Mukawa,T., Matsui,J., Higashi,M., Shimizu,K.D: "Molecularly Imprinted Polymers with Metalloporphyrin-Based Molecular Recognition Sites Coassembled with Methacrylic Acid"Anal. Chem. 73. 3869-3874 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeuchi,T., Seko A., Matsui,J., Mukawa,T: "Molecularly Imprinted Polymer Library on a Microtiter Plate : High-throughput Synthesis and Assessment of Cinchona Alkaloid-imprinted Polymers"Instrument. Sci. Technol. 29. 1-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kugimiya,A., Mukawa,T., Takeuchi,T: "Synthesis of 5-Fluorouracil-Imprinted Polymers with Multiple Hydrogen Bonding Interaction"Analyst. 126. 772-774 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kugimiya,A., Kuwada,Y., Takeuchi,T: "Preparation of Sterol-Imprinted Polymers with the Use of 2- (Methacryloyloxy) ethyl Phosphate"J. Chromatogr. A. 938. 131-135 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kugimiya,A., Takeuchi,T: "Molecular Recognition in Indoleacetic Acid-Imprinted Polymers : Effect of 2-Hydroxyrthyl Melhacrylate Contents"Anal. Bioanal. Chem. 372. 305-307 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary

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Published: 2001-04-01   Modified: 2016-04-21  

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