| Project/Area Number |
13672281
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
YAMAMOTO Hiroshi Osaka University, Graduate School of Pharmaceutical Sciences, Professor, 薬学研究科, 教授 (50127312)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIKAWA Kazutake Osaka University, Graduate School of Pharmaceutical Sciences, Research Associate, 薬学研究科, 助手 (10207376)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | fetal liver / thymus / stromal cells / hematopoiesis / immature thymocytes / growth factor / 胸腺間質細胞 |
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| Research Abstract |
Development, growth, and maintenance of hematopoietic cells in the fetal liver or lymphocytes in the thymus are regulated various kinds of cellular interaction molecules. To investigate these molecules, we developed novel co-culture methods, i.e, co-culturing fetal liver adherent cells and non-adherent cells. Applying this method, we established a novel mAb detecting CD13 molecule on the fetal liver adherent cells. CD13 is also acting on the development of fetal thymocytes. On the other hand, we identified that saposin, a molecule regulating sphingomyelin metabolism, is acting as a growth factor of immature thymocytes.
(1) A novel mAb, Ndk-10, inhibited the c-kit+ cell proliferation in the fetal liver. Amino acid sequence study revealed that the Ndk-10 reacts with CD 13.
(2) CD13 is also expressed in the early thymus and thymic medullary epithelial cells of adult mice. CD13 expression was enhanced by the addition of IL-4.
(3) Ndk-10 (anti-CD13) also inhibited c-kit+ cell proliferation in the fetal liver organ culture system.
(4) We purified immature thymocyte growth factor from Con-A stimulated splenocyte culture, and its partial amino acid sequence was found to be identical to that of saposin.
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