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Studies on the mechanisms underlying renal tubular deficiency due to the lack of the expression and function of CLCN5, a gene responsible for Dent's disease

Research Project

Project/Area Number 13672287
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

TAKANO Mikihisa  Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (20211336)

Co-Investigator(Kenkyū-buntansha) NAGAI Junya  Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (20301301)
MURAKAMI Teruo  Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (20136055)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsDent's disease / chloride channel / ClC-5 / endocytosis / proteinuria / hypercalciuria / receptor / renal tubules / 高カルシウム尿症 / 培養腎上皮細胞 / エンドソーム内酸性化 / 蛋白尿 / 遺伝性腎疾患
Research Abstract

Dent's disease is a X-linked recessive nephrolithiasis associated with various renal dysfunctions such as proteinuria and hypercalciuria. Recently, CLCN5, a gene encoding chloride channel ClC-5, was found to be responsible for Dent's disease, though the precise role of ClC-5 is not known at this moment. ClC-5 is highly expressed in the kidney, where the channel is involved in the acidification of endosomes, an important step for receptor-mediated endocytosis. On the other hand, proteins filtered through glomerulus are efficiently taken up by tubular cells by receptor-mediated endocytosis. In the present study, I examined the role of ClC-5 in renal handling of proteins and calcium.
Cultured renal epithelial cells OK were used as a model system, which I confirmed the expression of ClC-5. When cells were treated with chloride channel inhibitors, the endocytosis of FITC-labeled albumin was inhibited. In addition, the uptake of calcium by OK cells was inhibited by chloride channel inhibitors as well as by anti-ClC-5 antibody. Therefore, C1C-5 would have an important role for the endocytosis of filtered protein and calcium uptake in the kidney. These results may explain the proteinuria and hypercalciuria observed in Dent's disease, in which ClC-5 is lacking.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Sasaki, Y. et al.: "Expression of chloride channel, ClC-5, and its role in receptor-mediated endocytosis of albumin in OK cells"Biochem. Biophys. Res. Commun.. 282. 212-218 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagai, J. et al.: "Role of megalin in the renal handling of aminoglycosides"Am. J. Physiol.. 281. F337-F344 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Murakami, T. et al.: "Factors affecting the expression and function of P-glycoprotein in rats : drug treatments and diseased states"Pharmazie. 57. 102-107 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, M. et al.: "Cisplatin-induced inhibition of receptor-mediated endocytosis of protein in the kidney"Kidney Int.. 62. 1707-1717 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagai, J. et al.: "Effect of gentamicin on pharmacokinetics of lysozyme in rat : interaction between megalin substrates in the kidney"J. Pharm. Pharmacol.. 54. 1491-1496 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, Y. et al.: "Expression of chloride channel, ClC-5, and its role in receptor-mediated endocytosis of albumin in OK cells"Biochem.Biophys.Res.Commun.. 282. 212-218 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagai, J. et al.: "Role of megalin in the renal handling of aminoglycosides"Am.J.Physiol.. 281. F334-F344 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Murakami, T. et al.: "Factors affecting the expression and function of P-glycoprotein in rats : drug treatments and diseased states"Pharmazie. 57. 102-107 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, M. et al.: "Cisplatin-induced inhibition of receptor-mediated endocytosis of protein in the kidney"Kidney Int.. 62. 1707-1717 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagai, J. et al.: "Effect of gentamicin on pharmacokinetics of lysozyme in rat : interaction between megalin substrates in the kidney"J.Pharm.Pharmacol.. 54. 1491-1496 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Maeda, Y. et al.: "Possible mechanism for pharmacokinetic interaction between lidocaine and mexiletine"Clin. Pharmacol. Ther.. 71・5. 389-397 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano, M. et al.: "Cisplatin-induced inhibition of receptor-mediated endocytosis of protein in the kidney"Kidney Int.. 62. 1707-1717 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Murakami, T. et al.: "Interaction of endogenous compounds in human and rat urine with P-glycoprotein"Drug Metabol. Pharmacokin.. 17・3. 245-252 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagai, J. et al.: "Effect of gentamicin on pharmacokinetics of lysozyme in rat : interaction between megalin substrates in the kidney"J. Pharm. Pharmacol.. 54. 1491-1496 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sasaki, Y. et al.: "Expression of chloride channel, ClC-5, and its role in receptor-mediated endocytosis of albumin in OK cells"Biochem. Biophys. Res. Commun. 282・1. 212-218 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagai, J. et al.: "Role of megalin in the renal handling of aminoglycosides"Am. J. Physiol. 281・2. F337-F344 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Huang, Z.-H.et al.: "Expression and function of P-glycoprotein in rats with carbon tetrachloride-induced acute hepatic failure"J. Pharm. Pharmacol. 53・6. 873-881 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Murakami, T. et al.: "Factors affecting the expression and function of P-glycoprotein in rats : drug treatments and diseased stares"Pharmazie. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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