• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Isolation of human antibodies targeting to immunocytes from phage library and regulation of immune response

Research Project

Project/Area Number 13672325
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 医薬分子機能学
Research InstitutionKagoshima University

Principal Investigator

ITO Yuji  Kagoshima University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (60223195)

Co-Investigator(Kenkyū-buntansha) SUGIMURA Kazuhisa  Kagoshima University, Faculty of Engineering, Professor, 工学部, 教授 (80127240)
NAKASHIMA Toshihiro  The Chemo-Sero-Therapeutic Research Institute, Research group leader, 研究室長
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsCostimulatory molecules / human antibody / phage library / vaccine / antigen presenting cell / T cell / antagonist / targeting / 免疫応答制御 / 単鎖Fv抗体 / T細胞増殖 / アレルギー / 拒絶反応抑制 / 抗体医薬 / 遺伝子治療 / ワクチン開発 / ファージディスプレイ / 阻害抗体 / 免疫反応の制御 / DNAワクチン
Research Abstract

The aims of this study are the isolation of human antibodies directed to the costimulatory molecules on the lymphocytes, the establishment of the targeting and the control of the immune system by using antibodies, and their applications for DNA vaccine development. We succeeded in the isolation of anti B7RP-1 antibodies which could inhibit the costimulatory signal between ICOS and B7RP-1 by human antibody phage display library and are now applying for a patent. The obtained three antibodies could actually repress the T cell proliferation which was induced by anti CD3 antibody stimulation. We also developed other human antibodies that were specific to CD86, a costimulator ligand for CD28 and are now under preparation for a patent. The antibodies mentioned here can function as not only antagonist blocking the costimulatory signal but also targeting molecule to the antigen presenting cells (APC), because APC express abundantly such CD86 and B7RP-1 molecules, and such targeting ability is very important for the effective vaccine development to lead the antigens efficiently to the APC. We are now investigating the new vaccine developing studies by use of our human antibodies. As a preliminary experiment, we constructed a gene of fusion protein of extracellular domain of CTLA-4 (targeting molecule to APC) and human Fc (antigen) and immunized it through skin on mice as DNA vaccine. Consequently high antibody response to the antigen was obtained in the case of the administration of CTLA-4-Fc fusion, as compared with the administration of Fc (antigen) only. These results indicate that the targeting of the antigen to the APC give the promising effect in the vaccine efficacy.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (37 results)

All 2004 2003 2002 2001 Other

All Journal Article (20 results) Book (1 results) Patent(Industrial Property Rights) (1 results) Publications (15 results)

  • [Journal Article] Affinity maturation of IL-6 mimic peptide IL-6/37 isolated from phage library2004

    • Author(s)
      (7)田中孝一(他, 6名)
    • Journal Title

      Peptide Science 2003 2003

      Pages: 347-350

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Molecular characterization of a 10-kDa buckwheat molecule reactive to allergic patient's IgE2004

    • Author(s)
      (8)松本亮一(他, 8名)
    • Journal Title

      Allergy 59

      Pages: 533-538

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Molecular characterization of a 10-kDa buckwheat molecule reactive to allergic patient's IgE2004

    • Author(s)
      R.Matsumoto, K.Fujino, Y.Nagata, S.Hashiguchi, Y.Ito, Y.Aihara, Y.Takahashi, K.Maeda, K.Sugimura
    • Journal Title

      Allergy Vol.59

      Pages: 533-538

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Peptide Phage Library Based on The Tertiary Structure of Binding Region on Immuno-receptor2003

    • Author(s)
      (4)坂元孝太郎(他, 4名)
    • Journal Title

      Peptide Science 2002 2002

      Pages: 279-280

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Human FcεRIα-Specific Human Single-Chain Fv(scFv) Antibody with Antagonistic Activity to IgE/FcεRIα-Binding2003

    • Author(s)
      (5)橋口周平(他, 7名)
    • Journal Title

      J.Biochem. 133

      Pages: 43-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Human Hybridoma, Human single-chain Fv(scFv) antibody specific to human IL-6 with the inhibitory activity on IL-6-signaling2003

    • Author(s)
      (6)源島龍(他, 6名)
    • Journal Title

      Human Antibodies 11

      Pages: 121-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 抗体エンジニアリングの最前線:ヒト抗体エンジニアリングと分子標的医療2003

    • Author(s)
      (10)伊東祐二(他, 3名)
    • Journal Title

      BIOindustry 20(7)

      Pages: 34-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] ファージディスプレイ法2003

    • Author(s)
      (11)杉村和久(他, 2名)
    • Journal Title

      Molecular Medicine 40(10)

      Pages: 1150-1158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Human Fc ε RI α - Specific Human Single-Chain Fv (scFv) Antibody with Antagonistic Activity toward IgE/Fc ε RI α -Binding2003

    • Author(s)
      S.Hashiguchi, T.Nakashima, A.Nitani, T.Yoshihara, K.Yoshinaga, Y.Ito, Y.Maeda K.Sugimura
    • Journal Title

      J.Biochem. 133

      Pages: 43-49

    • NAID

      50000738710

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Human single-chain Fv (scFv) antibody specific to human IL-6 with the inhibitory activity on IL-6-signaling2003

    • Author(s)
      R.Gejima, K.Tanaka, T.Nakashima, S.Hashiguchi, Y.Ito, K.Yoshizaki, K.Sugimura
    • Journal Title

      Human Antibodies 11

      Pages: 121-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Affinity maturation of IL-6 mimic peptide IL-6/37 isolated from phage library2003

    • Author(s)
      K.Tanaka, A.Miyazaki, K.Yoshinaga, S.Hashiguchi, Y.Ito, K.Yoshizaki, K.Sugimura
    • Journal Title

      Peptide Science 2003

      Pages: 347-350

    • NAID

      10012654364

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Structure-activity Relationship of Cytokine-mimic Peptide from Phage Library2002

    • Author(s)
      (3)伊東祐二(他, 6名)
    • Journal Title

      Peptide Science 2001 2001

      Pages: 113-116

    • NAID

      10009660265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] ファージディスレイライブラリ法〜原理から最新技術まで〜2002

    • Author(s)
      (9)伊東祐二
    • Journal Title

      バイオベンチャー 2(4)

      Pages: 51-58

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Enhanced immunogenicity of a single-dose antigen based on nanospheres with antigen presenting cell-targeting function2002

    • Author(s)
      Y.Ito, Y.Kajiwara, A.Kimura, S.Hashiguchi, M.Akashi, K.Sugimura
    • Journal Title

      Peptides 2002

      Pages: 520-521

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Peptide phage library based on the tertiary structure of binding region on immuno-receptor2002

    • Author(s)
      Kotaro Sakamoto, Yuji Ito, Keisuke Yoshinaga, Shuhei Hashiguchi, Kazuhisa Sugimura
    • Journal Title

      Peptide Science 2002

      Pages: 279-280

    • NAID

      80015974583

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Peptide mimics of monocyte chemoattractant protein-1(MCP-1) with an antagonistic activity2001

    • Author(s)
      (1)加治正知(他, 7名)
    • Journal Title

      Journal of Biochemistry 129

      Pages: 577-583

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] DnaA protein Lys-415 is close to the ATP-binding site : ATP-pyridoxal affinity labeling2001

    • Author(s)
      (2)窪田敏夫(他, 4名)
    • Journal Title

      Biochemical and Biophysical Research Communication 288

      Pages: 1141-1148

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Peptide Mimics of Monocyte Chemoattractant Protein-1 (MCP-1) with an Antagonistic Activity2001

    • Author(s)
      Masatomo Kaji, Masaomi Ikari, Shuhei Hashiguchi, Yuji Ito, Ryo Matsumoto, Teizo Yoshimura, Jun-ichi Kuratsu, Kazuhisa Sugimura
    • Journal Title

      J.Biochem. 129

      Pages: 577-583

    • NAID

      10007854595

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] DnaA protein Lys-415 is close to the ATP-binding site : ATP-pyridoxal affinity labeling2001

    • Author(s)
      Toshio Kubota, Yuji Ito, Kazuhisa Sekimizu, Mitsuo Tagaya, Tsutomu Katayama
    • Journal Title

      Biochem Biophys Res Commun 288

      Pages: 1141-1148

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Structure-activity Relationship of Cytokine-mimic Peptide from Phage Library2001

    • Author(s)
      Yuji Ito, Ryu Gejima, Satoko Okuhara^*, Ryo Matsumoto, Shuhei Hashiguchi, Kazuyuki Yoshizaki^*, Kazuhisa Sugimura
    • Journal Title

      Peptide Science 2001

      Pages: 113-116

    • NAID

      10009660265

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Book] 蛋白質研究のための実験マニュアル:ヒト抗体をディスプレイするバクテリオファージ(分担執筆)2004

    • Author(s)
      伊東祐二(他, 2名)
    • Total Pages
      194
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Patent(Industrial Property Rights)] ヒト抗ヒトB7RP-1抗体、該抗体フラグメント、及びその利用方法2004

    • Inventor(s)
      伊東祐二, 他3名
    • Industrial Property Rights Holder
      内野 矜自
    • Industrial Property Number
      2004-198224
    • Filing Date
      2004-07-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 田中孝一: "Affinity maturation of IL-6 mimic peptide IL-6/37 isolated from phage library"Peptide Science 2003. 347-350 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 伊東祐二: "ヒト抗体エンジニアリングと分子標的医療"バイオインダストリー. 20巻7号. 34-42 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 杉村和久: "ファージディスプレイ法"Molecular Medicine. Vol.40(10). 1150-1158 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 伊東祐二: "抗体エンジニアリングの最前線(分担)"シーエムシー出版. 194(9) (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 伊東祐二: "タンパク質研究のための抗体実験マニュアル(分担)"羊土社. 122(7) (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 橋口周平(他、7名): "Human FcεRIα-Specific Human Single-Chain Fv (scFv) Antibody with Antagonistic Activity to IgE/FcεRIα-Binding"J.Biochem.. 133. 43-49 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 源島龍(他、6名): "Human Hybridoma, Human single-chain Fv (scFv) antibody specific to human IL-6 with the inhibitory activity on IL-6-signaling"HUMAN ANTIBODIES & HYBRIDOMAS. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] 伊東祐二: "本格化する抗体医療〜ファージディスプレイライブラリ法〜"バイオベンチャー. 2・4. 51-58 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 坂元孝太郎(他、4名): "Peptide Phage Library Based on The Tertiary Structure of Binding Region on Immuno-receptor"Peptide Science 2002. 2002(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 加治正知 (他, 7名): "Peptide mimics of monocyte chemoattractant protein-1(MCP-1)with an antagonistic activity"Journal of Biochemistry. 129. 577-583 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 杉村和久 (他, 7名): "IL-6-binding motif peptides inhibiting the signaling of human IL-6 to human 1L-6 receptor but not murine IL-6 receptor"Scandinavian Journal of Immunology. 54. S105 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 橋口周平 (他, 4名): "Human FcεRIα-specific human single chain Fv(scFv)antibody"Scandinavian Journal of Immunology. 54. S103 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 窪田敏夫 (他, 4名): "DnaA protein Lys-415 is close to the ATP-binding site : ATP-pyridoxal affinity labeling"Biochemical and Biophysical Research Communication. 288. 1141-1148 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 杉村和久 (他, 8名): "Peptide mimics of monocyte chemoattractant protein-1(MCP-1)with an antagonistic activity"Scandinavian Journal of Immunology. 54. S105 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 伊東祐二 (他, 6名): "Structure-activity Relationship of Cytokine-mimic Peptide from Phage Library"Peptide Science 2001. 2001. 113-116 (2002)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi