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Study on clinical significance of a new acute inflammatory protein (MRP8/14) as a useful marker for inflammatory responses associated with transplant rejection

Research Project

Project/Area Number 13672419
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

IKEMOTO Masaki  College of Medical Technology, Kyoto University, Associated professor, 医療技術短期大学部, 助教授 (80144385)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsArginase / MRP8 / 14 / Inflammation / Rejection / Leukocytes / Transplantation / Liver / Macrophage / 抗菌作用 / MRP / 小腸
Research Abstract

We reported so far that human liver-type arginase leaked out of liver cells upon hepatocellular damage. Recently, we first found that the liver-type arginase binds with myeloid-related protein (MRP8/14) composed of two different type subunits, MRP8 and MRP14. To assess the clinical significance of MRP8/14, we purified the MRP8/14 protein in human leukocytes and then prepared some monoclonal antibodies for the MRP8/14 as described previously. Using these antibodies, we developed a specific and sensitive ELISA system for MRP8/14 and applied it to sera from recipients of liver and small intestine transplantation, and further examined its usefulness as a new acute inflammatory marker. We observed a marked increase in serum MRP8/14 postoperatively in most recipients of transplants, followed by an increase in C-reactive protein (CRP) 1-7 days after the increase in the complex. The increase in serum MRP8/14 occurred simultaneously wit permeation of lymphocytes into the transplanted tissues as a result of rejection of the graft tissues. Thus, serum MRP8/14 seemed to be more sensitive for inflammatory responses associated with transplant rejection than was CRP. Changes in the serum level of MRP8/14 should be clinically useful for detecting acute inflammation occurred somewhere in the body. In conclusion, MRP8/14 is better than CRP as a acute inflammatory marker

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 池本正生, 恒川昭二, 笠井泰成, 田中紘一, 上田國寛, 戸谷誠之: "肝移植後のアルギナーゼ抗体の意義"臨床病理. Vol.49(7). 651-656 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Ikemoto, S.Tsunekawa, Y.Toda, M.Totani: "Liver-type arginase is a highly sensitive marker for hepatocellular damage in rats"Clinical Chemistry. Vol.47(5). 946-948 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Ikemoto, S.Tsunekawa, M.Awane, Y.Fukuda, et al.: "A useful ELISA system for human liver-type arginase, and its utility in diagnosis of liver diseases"Clinical Biochemistry. Vol.34. 455-461 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Miyamoto, N.Nagaya, M.Ikemoto, K.Tambara, et al.: "Elevation of matrix metalloproteinase-2 level in pericardial fluid is closely associated with left ventricular remodeling"The American Journal of Cardiology. Vol.89(1). 49-53 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Kimura, K.Tasumi, H.Tada, Y.Izumi, A.Kaneko, et al.: "Anti-CYP2D6 antibodies detected by quantitative radiolig and assay and relation to antibodies to liver-specific arginase in Japanese patients....."Clinica Chimica Acta. Vol.316. 155-164 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Ikemoto, T.Tanaka, Y.Takai, H.Murayama, K.Tanaka, M.Fujita: "A New ELISA System for Myeloid-Related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory....."Clinical Chemistry. Vol.49(4). 594-600 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaki,Ikemoto et al.: "Clinical Significance of Anti-Liver-Type Arginase Autoantibody in Blood of Recipients after Partial Liver Transplantation (in Japanese)"Rinsho Byori. vol. 49-7. 651-656 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M,Ikemoto et al.: "Liver-type arginase is a highly sensitive marker for hepatocellular damage in rats"Clinical Chemistry. vol. 47-5. 946-948 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M,Ikemoto et al.: "A useful ELISA system for human liver-type arginase, and its utility in diagnosis of liver diseases"Clinical Biochemistry. vol.34. 455-461 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S,Miyamoto et al.: "Elevation of matrix metalloproteinase-2 level in pericardial fluid is closely associated with left ventricular remodeling"The American Journal of Cardiology. vol. 89-1. 49-53 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M,Kimura et al.: "Anti-CYP2D6 antibodies detected by quantitative radioligand assay and relation to antibodies to liver-specific arginase in patients with autoimmune hepatitis"Clinica Chiimica Acta. vol. 316. 155-164 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M,Ikemoto et al.: "New ELISA System for Myelold-related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory Responses Associated with Transplant Rejection"Clinical Chemistry. vol. 49-4. 594-600 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 池本正生, 恒川昭二, 笠井泰成, 田中紘一, 上田國寛, 戸谷誠之: "肝移植後のアルギナーゼ抗体の意義"臨床病理. Vol.49(7). 651-656 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Ikemoto, S.Tsunekawa, Y.Toda, M.Totani: "Liver-type arginase is a highly sensitive marker for hepatocellular damage in rats"Clinical Chemistry. Vol.47(5). 946-948 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Ikemoto, S.Tsunekawa, M.Awane, Y.Fukuda, et al.: "A useful ELISA system for human liver-type arginase, and its utility in diagnosis of liver diseases"Clinical Biochemistry. Vol.34. 455-461 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Miyamoto, N.Nagaya, M.Ikemoto, K.Tambara, et al.: "Elevation of matrix metalloproteinase-2 level in pericardial fluid is closely associated with left ventricular remodeling"The American Journal of Cardiology. Vol.89(1). 49-53 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Kimura, K.Tasumi, H.Tada, Y.Izumi, A.Kaneko, et al.: "Anti-CYP2D6 antibodies detected by quantitative radiolig and assay and relation to antibodies to liver-specific arginase in Japanese patients....."Clinica Chimica Acta. Vol.316. 155-164 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Ikemoto, T.Tanaka, Y.Takai, H.Murayama, K.Tanaka, M.Fujita: "A New ELISA System for Myeloid-Related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory...."Clinical Chemistry. Vol.49(4)(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 池本正生, 恒川昭二, 笠井泰成, 田中紘一, 上田國寛, 戸谷誠之: "肝移植後のアルギナーゼ抗体の意義"臨床病理. 49・7. 651-656 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Ikemoto, S Tsunekawa Y.Toda, M.Totani: "Liver-type arginase is a highly sensitive marker for hepatocellular damage in rats"Clinical Chemistry. 47・5. 946-948 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Ikemoto, S.Tsunekawa M.Awane, Y.Fukuda, et al.: "A useful ELISA system for human liver-type arginase, and its utility in diagnosis of liver diseases"Clinical Biochemistry. 34. 455-461 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Miyamoto, N.Nagaya, M.Ikemoto, K.Tambara, et al.: "Elevation of matrix metalloproteinase-2 level in pericardial fluid is closely associated with left ventricular remodeling"The American Journal of Cardiology. 89・1. 49-53 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Kimura, K.Tasumi, H.Tada, Y.Izumi, A.Kaneko, et al.: "Anti-CYP2D6 antibodies detected by quantitative radiolig and assay and relation to antibodies to liver-specific arginase in Japanese patients"Clinica Chimica Acta. 316. 155-164 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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