|Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
To isolate novel transcripts expressed specifically in this gland, identified three novel pituitary-specific transcripts which encode putative proteins: pituitary gland specific factor 1a (PGSF1a), PGSF1b and PGSF2 through generating pituitary BodyMap database. To understand the pathogenesis of lymphocytic hypophysitis and to diagnose this disease efficiently, we developed laboratory testing methods. By developing radioligand assay using recombinant human 35S-labeled protein, presence of autoantibodies against three pituitary-specific proteins, GH, PGSF1a and PGSF2, and 5 enzymes expressed in the pituitary gland, prohormone convertase (PC) 1/3, PC2, carboxypeptidase E (CPE), alpha enolase, and PC2 regulatory protein, 7B2 were analyzed. Anti-human GH, anti-PGSF1a, and anti-PGSF2 antibodies (Ab) were detected in patients with lymphocytic hypophysitis and other hypopituitarism, but were not detected in patients with non-functioning pituitary macroadenoma. Patients positive for either anti-PC1/3 or anti-7B2 Ab were significantly frequent among patients with nonfunctioning pituitary macroadenoma than in other pituitary diseases and healthy controls. None of the patients was positive for anti-PC2 Ab or anti-CPE Ab. Anti-alpha-enolase Ab were not suitable for specific diagnosis of lymphocytic hypophysitis or pituitary non-functioning adenoma. Detection of antibodies against GH, PGSF1a, PGSF2, PC1/3 and 7B2 may be useful for the diagnosis of lymphocytic hypophysitis.