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ESTABLISHMENT OF QUANTITATIVE MEASUREMENT OF FRAGMENTED RED CELLS USING RETICULOCYTE ANALYZER

Research Project

Project/Area Number 13672424
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKOBE UNIVERSITY

Principal Investigator

SAIGO Katsuyasu  Kobe University, University Hospital Lecturer, 医学部附属病院, 講師 (20304107)

Co-Investigator(Kenkyū-buntansha) KUMAGAI Shunichi  Kobe University, Graduate School of Medicine Professor, 医学部附属病院, 教授 (00153346)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsfragmented red cells / automated hematology analyzer / XE-2100 / hemolytic uremic syndrome / thrombotic thrombocytopenic purpura / LD isoenzyme / bone marrow transplantation-relaed thrombotic microangiopathy / 自動血球測定装置 / 溶血性尿毒症症候群 / 血栓性血小板減少性紫斑病症 / 移植後血栓性微少血管障害 / 血管炎 / トロンボモジュリン
Research Abstract

Purpose : The purposes were to establish a method of fragmented red cells (FRC) quantification using automated hematology analyzer, XE-2100 and to study about the clinical usefulness of this method.
Research ptocess : Experimentary produced FRC by heating were used and new program which shows positive correlation with the results of manual counting has been established. As the patients having small red cells such as iron deficiency anemia shows false pocitive data, complementary gate to revise the results was also developed. Now, clinical study to evaluate its efficacy of the diagnosis of bone marrow transplantation-related thrombotic microangiopathy (BMT-TMA) in collaboration with three other hospitals in Hyogo.
Results :
1) Reticulocyte analyzer (RET channel) equipped to XE-2100 analyzes cells by flowcytometry after staining with fluorescent dye. In the RET channel, small red cells were considered as FRC, and gate 1 was set to positively refiect the manual counting data (r=0.986). Ninety clinical samples also showed significantly positive correlation between them (r=0.876, p<0.001). However, 17 samples obtained from patients with small MCV had falsely positive FRC data due to detection nomal small cells in gate 1. Then, we have developed complementary gate 2 and the following formula was obtained. Revised FRC=calculated FRC/e 0.43^<gate2/total>
2) The usefulness of this method was evaluated in the 14 patients with hemolytic uremic syndrome or thrombotic thrombocytopenic purpura. FRC did not correlated with total LD on admission. As increase in LD isoenzyme 5 was observed, we have checked the relationship between them (r=0.732, p=0.008), indicating that FRC detected by our method reflects hemolysis.
3) The increase in FRC % in the patients treated with bone marrow transplantation was detected in several conditions such as infection, VOD and so, on. Now, weare evaluating the efficacy of this method to detect BMT-TMA.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Jiang M., Saigo, K., et al.: "Quantification of red cell fragmentation by automatic hematology analyzer XE-2100"Clinical and Laboratory Haematology. 23. 167-172 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saigo, K., Kumagai, S., et al.: "RANTES and p-selectin in peripheral blood stem cell harvest"Therapeutic Apheresis. 5. 517-518 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asahara, S., Saigo, K., et al.: "Acute lymphoblastic leukemia accompanied by chromosomal abnormality of translocation (12;17)"Haematologia. 31. 209-213 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saigo, K., Sugimoto, T., et al.: "Fluctuations in plasma macrophage colony-stimulating factor levels during autologous peripheral blood stem cell transplantation for hematologic diseases"Clinical and Laboratory Haematology. 24. 37-42 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saigo, K., Jiang, M., et al.: "Usefulness of automatic detection of fragmented red cells using a hematology analyzer for diagnosis of thrombotic microangiopathy"Clinical and Laboratory Haematology. 24. 347-351 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saigo, K., Kogi, M et al.: "Acute B-cell lymphoblastic leukemia with translocation (14;18) : Establishment of a new cell line"Cancer Genetics and Cytogenetics. 140. 174-175 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jiang,M., Saigo,K., Kumagai,S., Imoto,S., Kosaka Y., Matsumoto,H., Fujimoto,K: "Quantification of red cell fragmentation by automatic hematology analyzer XE-2100"Clinical and Laboratory Haematology. 23(3). 167-172 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saigo,k., Kumagai,S., Sugimoto,T., Matsui,T., Ryo,R: "RANTES and p-selectin in peripheral blood stem cell harvest"Therapeutic Apheresis. 5. 517-518 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asahara,S., Saigo,K., Hasuike,N., Tamura,M., Maeda,Y., Tomofuji,Y., Chinzei,T., Tatsumi,E: "Acute lymphoblastic leukemia accompanied by chromosomal abnormality of translocation (12 ; 17)"Haematologia. 31. 209-213 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saigo,K., Sugimoto,T., Matsui,T., Ryo,R., Kumagai,S: "Fluctuations in plasma macrophage colony-stimulating factor levels during autologous peripheral blood stem cell transplantation for hematologic diseases"Clinical and Laboratory Haematology. 24. 37-42 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saigo,K., Jiang,M., Tanaka,C., Fujimoto,K., Kobayashi,A., Nozu,K., Iijima,K., Ryo,R., Sugimoto,T., Imoto,S., Kumagai,S: "Usefulness of automatic detection of fragmented red cells using a hematology analyzer for diagnosis of thrombotic microangiopathy"Clinical and Laboratory Haematology. 24. 347-351 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saigo,K, Kogi,M, Imoto,S, Hamano,K, Morita,S, Chinzei,T, Koizumi,T, Tatsumi,E, Kumagai,S: "Acute B-cell lymphoblastic leukemia with translocation (14 ; 18) : Establishment of a new cell line"Cancer Genetics and Cytogenetics. 140. 174-175 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaike, N., Saigo, K., et al.: "Autoimmune hepatitis accompanied by idiopathic thrombocytopenic purpura and Sjogren's syndrome"Internal Medicine. 41. 72 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamamoto, M., Saigo, K., et al.: "Inhibition of natural killer cytotoxicity in vitro by clinical grade serine protease inhibitors"Haematologai. 32. 103-112 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saigo, K., et al.: "Usefulness of automatic detection of fragmented red cells using a hematology analyzer for diagnosis of thrombotic microangiopathy"Clinical and Laboratory Haematology. 24. 347-351 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saigo, K., et al.: "Acute B-cell lymphoblastic leukemia with translocation (14;18) : Establishment of a new cell line"Cancer Genetics and Cytogenetics. 140. 174-175 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saigo, K., et al.: "Platelet and RANTES contamination in peripheral blood stem cell (PBSC) products : comparison of three different instruments for PBSC harvesting"Vox Sanguinis. (In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 森田志保, 西郷勝康ほか: "芽球に顆粒を有する急性リンパ性白血病(ALL)の2例"臨床病理. 50. 625-629 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Jiang, M.: "Quantification of red cell fragmentation by automatic hematology analyzer XE-2100"Clinical and Laboratory Haematology. 23. 167-172 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saigo.k.: "RANTES and p-selectin in peripheral blood stern cell harvest"Therapeutic Apheresis. 5. 517-518 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tatsumi, E.: "Induction of CD13 antigen in in vitro culture and assessment of negatine or positive in AML cases"American Journal of Clinical Pathology. 116. 945-947 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saigo, k.: "Fluctuations in plasma macrophage colony-stimulating factor levels during autologous peripheral blood stem cell transplantation for hematologic diseases"Clinical and Laboratory Haematology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 野村努: "多項目自動血球分析装置SE-9000のIMIチャンネルを用いた造血幹細胞測定条件の検討"日本輸血学会雑誌. 47. 639-645 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 東口佳苗: "全身性エリテマトーデス症例に出現した一過性の偽性白血球減少症"日本検査血液学会雑誌. (印刷中).

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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