Effects of endurance training with hypoxic stimulation induced by Co^<2+> treatment on oxygen transport system in rat skeletal muscles
| Project/Area Number |
13680011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
体育学
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| Research Institution | Hokkaido University of Education |
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Principal Investigator |
SUZUKI Junichi Research and Education Center for Winter Sports, Associate Professor, 冬季スポーツ教育研究センター, 助教授 (80261379)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | angiogenesis / training / hypoxia / cobalt / enzyme activity / skeletal muscle / endurance capacity / 酸素活性 |
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| Research Abstract |
This study was designed to examine the changes in the capillary geometry and expression of angiogenic growth factor in skeletal muscles after endurance training with and without chronic CoCl_2 administration. The Co^<2+>-treated rats drank water containing 0.01% CoCl_2. In the first experiment, training and Co^<2+> administration lasted for 5 weeks. Chronic Co^<2+> administration significantly increased the blood hemoglobin concentration by approximately 25% with and without training. Only in the diaphragm, the Co^<2+> treatment alone significantly increased total capillary density and the capillary-to-fiber ratio (C:F). Both training groups with and without Co^<2+> administration significantly increased the C:F in soleus (SOL) and plantaris (PL) muscles. In PL, the increase was significantly greater in the Co^<2+>-treated training group than in the non-Co^<2+>-treated training group. The densities of VEGF-positive capillaries (VEGF-cap) remained unchanged in all muscle portions examin
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ed after either Co^<2+> administration or exercise training. The second experiment was designed to examine the time course changes in VEGF expression and capillary geometry in the early stage of the treatment. After 6 and 10 days of the Co^<2+> administration, the VEGF-cap was significantly increased by 27% and 65%, respectively in SOL. After 10 days of Co^<2+> administration, the C:F was significantly increased. The training with Co^<2+> significantly increased the VEGF-cap by 69%, 44% and 60%, respectively, after 3, 6 and 10 days in SOL, while it significantly increased the VEGF-cap after 10 days in the mixed fiber portion of plantaris muscle (PLm). Training with or without Co^<2+> significantly increased the C:F after 6 and 10 days in the SOL. Training with Co^<2+>, but training alone did not, significantly increased the C:F in PLm after 10 days. In condition, the present results suggest that activation of the cellular oxygen-sensing mechanism induced by Co^<2+> administration facilitates microvascular remodeling in hind-leg muscles by facilitating an expression of VEGF. Less
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Report
(3 results)
Research Products
(12 results)
