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The effect of antioxidants on sarcoplasmic reticulum function during muscle fatigue

Research Project

Project/Area Number 13680030
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 体育学
Research InstitutionHiroshima University

Principal Investigator

WADA Masanobu  Hiroshima University, Faculty of Integrated Arts and Sciences, Associate Professor, 総合科学部, 助教授 (80220961)

Co-Investigator(Kenkyū-buntansha) HASEGAWA Hiroshi  Hiroshima University, Faculty of Integrated Arts and Sciences, Research Associate, 総合科学部, 助手 (70314713)
YASUDA Toshihiro  Fukushima University, Faculty of Education, Associate Professor, 教育学部, 助教授 (50323184)
MATSUNAGA Satoshi  Osaka City University, Institute for Health and Sports Sciences, Assistant Professor, 体育学研究室, 講師 (70221588)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordssarcoplasmic reticulum / fatigue / antioxidant / reactive oxygen species / calcium pump / oxidation / カルシウム / 筋収縮 / 運動 / 酸化 / Ca^<2+>取り込み速度 / Ca^<2+>放出速度
Research Abstract

The purpose of the first experiment was to examine a cause of exercise-induced decreases in Ca^<2+>-sequestering capacity by sarcoplasmic reticulum (SR) using the superficial portions of the gastrocnemius and vastus lateralis muscles of the rat after treadmill running to exhaustion. Exercise led to 16% and 34% decreases in SR Ca^<2+>-ATPase activity and Ca^<2+> uptake rate, respectively. The carbonyl group content in SR Ca^<2+>-ATPase protein was increased by 127%. In the second experiment, the hypothesis was tested that N-acetylcysteine (NAC) that is one of antioxidants may influence diaphragmic fatigue by modulating SR Ca^<2+> handling capacity. In the absence or presence of NAG, rat diaphragm was electrically stimulated with titanic trains (350 msec) delivered at 0.5 train/sec for various durations. At 60 sec after the onset of stimulation and beyond, force developed by NAC-treated muscle was significantly greater that that of non-treated muscle. In muscles where force was reduced to 50-80% of the initial level, treatment of NAG inhibited reductions in SR function. These results suggest that oxidation of SR Ca^<2+> -ATPase protein may account for decreases in Ca^<2+> -sequestering capacity by the SR and that antioxidant-induced inhibition of a decline in SR function may be responsible, at least partly, for a delay of the rate of fatigue development in the middle phase of muscular fatigue.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 三島隆章: "N-acetylcysteineが筋疲労時の横隔膜筋小胞体に及ぼす影響"広島体育学研究. 29. 27-34 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoshi Matsunaga: "Oxidation of SR Ca^<2+>-ATPase induced by high-intensity exercise"Pflugers Archives. 446. 334-399 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takaaki, Mishima: "Influence of N-acetylcysteine on sarcoplasmic reticulum function in fatigued diaphragm"Hiroshima Journal of Physical Education. 27-34 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoshi, Matsunaga: "Oxidation of sarcoplasmic reticulum Ca^<2+>-ATPase induced by high-intensity exercise"Pflugers Archives. 394-399 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 三島隆章ほか: "H-acetylcysteineが筋疲労時の横隔膜筋小胞体に及ぼす影響"広島体育学研究. 29. 27-34 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsunaga et al.: "Oxidation of SR Ca^<2+>-ATPase induced by high-intensity exercise"Pflugers Archiv. 446. 394-399 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsunaga, S. et al.: "Oxidative inactivation of sarcoplasmic reticulum Ca^<2+>-ATPase induced by high-intensity exercise"Pflugers Archiv. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 和田正信ほか: "筋小胞体の構造および筋活動に伴うその機能の変化"体育学研究. 46・5. 443-459 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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