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Docosahexaenoic acid intake potentiates antioxidative potential in vivo

Research Project

Project/Area Number 13680176
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食生活
Research InstitutionIncorporated Administrative Agency, National Institutes of Health and Nutrition

Principal Investigator

SAITO Morio  Incorporated Administrative Agency, National Institute of Health and Nutrition, Division of Food Science, Division Director, 食品機能研究部, 部長 (80196013)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsDocosahexaenoic acid / Lipid peroxides / Peroxidizability index / Glutathione / Acetaminophen / Detoxification / Phosphatidylethanolamine / Liposome / メチオニン / アスコルビン酸 / ビタミンE / 抗酸化作用 / フォスファチジルエタノールアミン
Research Abstract

In previous studies, we showed that docosahexaenoic acid (DHA) ingestion enhance the susceptibility of rat liver and kidney to lipid peroxidation but did not increase lipid peroxide formation to the level expected from the relative peroxidizability index (P-index) of the total tissue lipids. The results suggested the existence of some suppressive mechanisms against DHA-stimulated tissue lipid peroxide formation, as observed increased tissue ascorbic acid (AsA) and glutathione (GSH) levels. Furthermore, we have proposed that incorporation of docosahexaenoic acid (DHA) into phosphatidylethanolamine (PE) might enhance resistance to lipid peroxidation in vivo.
In this study, therefore, we focused initially on the roles of AsA and/or GSH for the suppressive mechanisms and secondly on the relationship between the transbilayer distribution of PE and oxidative stability of DHA combined with PE.
We examined the influences of different levels of dietary AsA and methionine, the latter of which is r … More equired for GSH-synthesis, on the liver lipid peroxide and antioxidant levels in vitamin C-requiring ODS rats. We found that physiological levels of AsA and methionine were required for suppressing DHA-stimulated lipid peroxide formation and consumption of vitamin E, but the dietary AsA and methionine over physiological levels did not suppress further the formation and consumption. In addition, when rats ingested a low level of methionine, liver lipid peroxide formation and glutathione-S-transferase activity were increased. These results suggested a limited antioxidant ability potentiated by AsA and GSH. Therefore, other factor such as detoxification of lipid peroxides by glutathione-S-transferase, may be related to the suppressive mechanisms.
In addition, to modulate the transbilayer distribution of PE (PE-DHA) obtained from the liver of rats fed DHA, we used phosphatidylcholine (PC) with two types of acyl chain moiety: dipalmitoyl (PC 16 : 0) and dioleoyl (PC 18 : 1). The proportion of PE-DHA in the liposomal external layer was significantly higher in liposomes containing PC18 : 1 than in those containing PC16 : 0. This tendency was more pronounced in liposomes extruded using a polycarbonate filter with smaller pore sizes. Additionally, PE-DHA in the external layer of liposomes prepared using a filter with smaller pore sizes could protect DHA itself from 2, 2' -azobis (2-amino-propane) dihydrochloride -mediated lipid peroxidation. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Wang JY, et al.: "Dietary supplementation of n-3 fatty acids and hydroperoxide levels in rat retinas"Free Radical Research. 35. 367-375 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saito M, et al.: "An assessment of docosahexaenoic acid intake from the viewpoint of safety and physiological efficacy in matured rats"Annals of Nutrition & Metabolism. 46. 176-181 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saito M, et al.: "Relationship between tissue lipid peroxidation and peroxidizability index after α-linolenic, eicosapentaenoic, or docosahexaenoic acid intake in rats"British Journal of Nutrition. 89. 19-28 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kubo K, et al.: "Docosahexaenoic acid-containing phosphatidylethanolamine in the external layer of liposomes protects docosahexaenoic acid from 2,2'-azobis(2-aminopropane)dihydrochloride-mediated lipid peroxidation"Archives of Biochemistry and Biophysics. 410. 141-148 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wang JY, et al.: "Effect of docosahexaenoic acid and ascorbate on peroxidation of retinal membranes of ODS rats"Free Radical Research. 37. 419-424 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wang JY et al.: "Dietary supplementation of n-3 fatty acids and hydroperoxide levels in rat retinas"Free Radical Research. 35. 367-375 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saito M et al.: "An assessment of docosahexaenoic acid intake from the viewpoint of safety and physiological efficacy in matured rats"Annals of Nutrition & Metabolism. 46. 176-181 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saito M et al.: "Relationship between tissue lipid peroxidation and peroxidizability index after α-linolenic, eicosapentaenoic, or docosahexaenoic acid intake in rats"British Journal of Nutrition. 89. 19-28 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kubo K et al.: "Docosahexaenoic acid-containing phosphatidylethanolamine in the external layer of liposomes protects docosahexaenoic acid from 2,2'-azobis(2-aminopropane)dihydrochloride-mediated lipid peroxidation"Archives of Biochemistry and Biophysics. 410. 141-148 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wang JY et al.: "Effect of docosahexaenoic acid and ascorbate on peroxidation of retinal membranes of ODS rats"Free Radical Research. 37. 419-424 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wang JY et al.: "Dietary supplementation of n-3 fatty acids and hydroperoxide levels in rat retinas"Free Radical Research. 35. 367-375 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saito M et al.: "An assessment of docosahexaenoic acid intake from the viewpoint of safety and physiological efficacy in matured rats"Annals of Nutrition & Metabolism. 46. 176-181 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saito M et al.: "Relationship between tissue lipid peroxidation and peroxidizability index after α-linolenic, eicosapentaenoic, or docosahexaenoic acid intake in rats"British Journal of Nutrition. 89. 19-28 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kubo K et al.: "Docosahexaenoic acid-containing phosphatidylethanolamine in the external layer of liposomes protects docosahexaenoic acid from 2,2'-azobis (2-aminopropane) dihydrochloride-mediated lipid peroxidation"Archives of Biochemistry and Biophysics. 410. 141-148 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Wang JY et al.: "Effect of docosahexaenoic acid and ascorbate on peroxidation of retinal membranes of ODS rats"Free Radical Research. 37. 419-424 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Wang JY, Saito M: "Dietary supplementation of n-3 fatty acids and hydroperoxide levels in rat retinas"Free Radical Res. 35. 367-375 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saito M, Kubo K: "An assessment of docosahexaenoic acid (DHA) intake from the viewpoint of safety and physiological efficacy in matured rats"Ann. Nutr. Metab.. 46(in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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