Analysis of C-terminal domain of ClassV myosin
| Project/Area Number |
13680707
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MATSUI Yasushi Graduate School of Sciences, Research Associate, 大学院・理学系研究科, 助手 (50229407)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | myosin / small GTPase / mitochondria / budding Yeast / クラスVミオシン / 極性 / 分配 / Myo2 / YTP11 |
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| Research Abstract |
We identified Ypt11p, a rab-type small GTPase, by its functional and two-hybrid interaction with Myo2p, a class V myosin of the budding yeast Saccharomyces cerevisiae. The tail domain of Myo2p was co-immunoprecipitated with Ypt11p, suggesting that Ypt11p forms a complex with Myo2p at its tail domain in vivo. Mutational analysis of YPT11 suggests that Myo2p is a putative effector of Ypt11p. Deletion of YPT11 induced partial delay of mitochondrial Transmission to the bud and overexpression of YPT11 resulted in mitochondrial accumulation in the bud, indicating that Ypt11p acts positively on mitochondrial distribution towards the bud. We isolated two myo2 mutants, myo2-338 and myo2-573, which showed genetic interactions with YPT11. The myo2-573 mutation, identified by synthetic lethal interaction with ypt11-null, induced a defect in mitochondrial distribution towards the bud, indicating that Myo2p plays a crucial role in polarized distribution of mitochondria. The myo2-338 mutation was identified as that abolished the effect of overexpressed YPT11, such as the Ypt11p-dependent accumulation of mitochondria in the bud, and was reduced the affinity of Myo2p for Ypt11p. These results indicate that complex formation of Ypt11p with Myo2p accelerates the Myo2p function for mitochondrial distribution towards the bud.
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Report
(3 results)
Research Products
(4 results)
