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Identification and functional analysis of new ribonuclease activated by death-receptor engagement

Research Project

Project/Area Number 13680727
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionNagoya University

Principal Investigator

NADANO Daita  Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (00228074)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsapoptosis / death receptor / ribonuclease / ribosomal RNA / ribosome / protein synthesis / ribosomal protein / cancer / 28SリボソームRNA / 腫瘍マーカー / ノックアウトマウス
Research Abstract

This project started at the purification of ribonuclease activity, which is activated by death-receptor engagement, cleaves 28S ribosomal RNA specifically, inhibits protein synthesis, and finally promotes apoptosis. Using the lysate of apoptotic Jurkat cells obtained by the stimulation of a death receptor, Fas, and the normal, isotope (^<32>P)-labelled ribosome, conditions for cell-free analysis system were examined, and thus it was succeeded to reproduce in vitro the in vivo observation of 28S ribosomal RNA degradation and to analyze extensively the properties of the ribonuclese. Then, this work faced on a serious problem that the ribonuclease activity was inseparable from other intracellular non-specific ribonuclease activities by conventional biochemical, chromatographic methods. To solve this problem and analyze the relationship between apoptosis and ribosome further, new strategy has been adopted to identify proteins interacting the ribosome specifically in apoptotic cells by 1)isolation of ribosome fraction from apoptotic and normalcells,2)electrophoresis of proteins in both fractions to reveal a difference in patterns of protein bands,3)analysis of apoptosis-specific protein bands using mass spectrometry. This approach is comprehensive and is expected to enable us to identify not only the ribonuclease cleaving 28S ribosomal RNA but also other proteins which specifically interact with the ribosome in apoptotis.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Nadano, D.(第一著者): "Electrophoretic characterization of ribosomal subunits and proteins in apoptosis : Specific down-regulation of S11 in human breast carcinoma treated with staurosporine."Biochemistry. 40. 15184-15193 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saburi, S.(第一著者): "The trophinin gene encodes a novel group of MAGE proteins, Magphinins, and regulates cell proliferation during gametogenesis in the mouse"Journal of Biological Chemistry. 276. 49378-49389 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura, M. T.(第一著者): "14-3-3 is involved in p75 neurotrophin receptor (p75^<NTR>) mediated signal transduction."Journal of Biological Chemistry. 276. 17291-17300 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nadano, D.(第一著者): "Significant differences between mouse and human trophinins are revealed by their expression patterns and targeted disruption of mouse trophinin gene."Biology of Reproduction. 66. 313-321 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishida, J.(第一著者): "Structural change of ribosomes during apoptosis : degradation and externalization of ribosomal proteins in doxorubicin-treated Jurkat cells."Journal of Biochemistry. 131. 485-493 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nadano, D.(第一著者): "A human gene encoding a protein homologous to ribosomal protein L39 is normally expressed in the testis and derepressed in multiple cancer cells."Biochimica et Biophysica Acta. 1577. 430-436 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nadano, D.(第一著者): "Human tastin, a proline-rich cytoplasmic protein, associates with the microtubular cytoskeleton."Biochemical Journal. 364. 669-677 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kasai, H.(第一著者): "Differential expression of ribosomal proteins in human normal and neoplastic colorectum."Journal of Histochemistry & Cytochemistry. 51. 567-573 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakayama, J.(第一著者): "Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies : possible role of human chorionic gonadotrophin on ectopic pregnancy."American Journal of Pathology. 163. 2211-2219 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nadano, D., Aoki, C., Yoshinaka, T., Irie, S., Sato, T.: "Electrophoretic characterization of ribosomal subunits and proteins in apoptosis : Specific down-regulation of S11 in human breast carcinoma treated with staurosporine."Biochemistry. 40(50). 15184-15193 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saburi, S., Nadano, D., Akama, T.O., Hirama, K., Yamanouchi, K., Naito, K., Tojo, H., Tachi, C., Fukuda, M.N.: "The trophinin gene encodes a novel group of MAGE proteins, Magphinins, and regulates cell proliferation during gametogenesis in the mouse."Journal Biological Chemistry. 276(52). 49378-49389 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura, M.T., Irie S., Shoji-Hoshino S., Mukai J., Nadano D., Oshimura M., Sato, T.: "14-3-3 is involved in p75 neurotrophin receptor (p75^<NTR>)-mediated signal transduction."Journal of Biological Chemistry. 276(20). 17291-17300 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nadano, D., Sugihara, K., Paria, B.C., Saburi, S., Copeland, N.G., Gilbert, D.J., Jenkins, N.A., Nakayama, J., Fukuda, M.N.: "Significant differences between mouse and human trophinins are revealed by their expression patterns and targeted disruption of mouse trophinin gene."Biology of Reproduction. 66(2). 313-321 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishida, J., Shiratsuchi, A., Nadano, D., Sato, T., Nakanishi, Y.: "Structural change of ribosomes during apoptosis : degradation and externalization of ribosomal proteins in doxorubicin-treated Jurkat cells."Journal of Biochemistry. 131(3). 485-493 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nadano, D., Notsu, T., Matsuda, T., Sato, T.: "A human gene encoding a protein homologous to ribosomal protein L39 is normally expressed in the testis and derepressed in multiple cancer cells."Biochimica et Biophysica Acta. 1577(3). 430-436 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nadano, D., Nakayama, J., Matsuzawa, S., Sato, T., Matsuda, T., Fukuda, M.N.: "Human tastin, a proline-rich cytoplasmic protein, associates with the microtubular cytoskeleton."Biochemical Journal. 364(3). 669-677 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kasai, H., Nadano, D., Hidaka, E., Higuchi, K., Kawakubo, M., Sato, T., Nakayama, J.: "Differential expression of ribosomal proteins in human normal and neoplastic colorectum."Journal of Histochemistry & Cytochemistry. 51(5). 567-573 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakayama, J., Aoki, D., Suga, T., Akama, T.O., Ishizone, S., Yamaguchi, H., Imakawa, K., Nadano, D., Fazleabas, A.T., Katsuyama, T., Nozawa, S., Fukuda, M.N.: "Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies : possible role of human chorionic gonadotrophin on ectopic pregnancy."American Journal of Pathology. 163(6). 2211-2219 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kasai, H.(第一著者): "Differential expression of ribosomal proteins in human normal and neoplastic colorectum"Journal of Histochemistry and Cytochemistry. 51・5. 567-573 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakayama, J.(第一著者): "Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies : possible role of human chorionic gonadotrophin on ectopic pregnancy"American Journal of Pathology. 163・6. 2211-2219 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nadano, D.(第一著者): "A human gene encoding a protein homologous to ribosomal protein L39 is normally expressed in the testis and derepressed in multiple cancer cells"Biochimica Biophysica Acta. 1577・3. 430-436 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nadano, D.(第一著者): "Human tastin, a proline-rich cytoplasmic protein, associates with the microtubular cytoskeleton"Biochemical Journal. 364・3. 669-677 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nadano, D. (第一著者): "Electrophoretic characterization of ribosomal subunits and proteins in apoptosis: Specific down-regulation of S11 in human breast carcinoma treated with staurosporine"Biochemistry. 40・50. 15184-15193 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nadano, D. (第一著者): "Significant differences between mouse and human trophinins are revealed by their expression patterns and targeted disruption of mouse trophinin gene"Biology of Reproduction. 66・2. 313-321 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishida, J. (第一著者): "Structural change of ribosomes during apoptosis: degradation and externalization of ribosomal proteins in doxorubicin-treated Jurkat cells"Journal of Biochemistry. 131・3. 485-493 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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