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Induction and detection of retroposition of SINEs and LINEs in animal genome

Research Project

Project/Area Number 13680759
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionTokyo Institute of Technology

Principal Investigator

OKADA Norihiro  Tokyo Institute of Technology, Graduate School of Bioscience & Biotechnology, Professor, 大学院・生命理工学研究科, 教授 (60132982)

Co-Investigator(Kenkyū-buntansha) OHSHIMA Kazuhiko  Tokyo Institute of Technology, Graduate School of Bioscience & Biotechnology, Lecturer, 大学院・生命理工学研究科, 講師 (60282852)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordstransposable elements / retroposons / retrotransposition / interspersed repetitive elments / genome / LINE / SW1 / レトロポジション / メダカ
Research Abstract

We characterized members of the LINE (UnaL2) and SINE (UnaSINE1) families from the eel genome and found that these LINE/SINE partners share similar 3' tails. A retrotransposition assay in HeLa cells demonstrated that the 3' conserved tail of UnaL2 is necessary for its retrotransposition. This 3' tail is recognized in trans by the UnaL2 reverse transcriptase at a surprisingly high rate, and that of UnaSINE1 can also be recognized, thus providing experimental evidence that a SINE can be mobilized by the retrotransposition machinery of a partner LINE. We also demonstrated that short repeats at the 3' end of UnaL2 are required for retrotransposition suggesting that UnaL2 retrotransposes in a manner reminiscent of the reverse transcriptase activity of telomerases.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Kajikawa, M., Okada, N.: "LINEs mobilize SINEs in the eel through a shared 3' sequence"Cell. 111. 433-444 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogiwara I, Miya M, Ohshima K, Okada N: "V-SINEs : a new superfamily of vertebrate SINEs that are widespread in vertebrate genomes and retain a strongly conserved segment within each repetitive unit"Genome Res.. 12. 316-324 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kajikawa, M., and Okada, N.: "LINEs mobilize SINEs in the eel through a shared 3' sequence"Cell. 111. 433-444 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogiwara I, Miya M, Ohshima K, Okada N.: "V-SINEs : a new superfamily of vertebrate SINEs that are widespread in vertebrate genomes and retain a strongly conserved segment within each repetitive unit"Genome Res.. 12. 316-324 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kajikawa, M., Okada, N.: "LINEs mobilize SINEs in the eel through a shared 3' sequence"Cell. 111. 433-444 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikuo Ogiwara, Masaki Miya, Kazuhiko Ohshima, Norihiro Okada: "V-SINEs : A New Superfamily of Vertebrate SINEs That Are Widespread in Vertebrate Genomes and Retain a Strongly Conserved Segment within Each Repetitive Unit"Genome Research. 12. 316-324 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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