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FUNCTION OF HUMAN TRNASCRIPTION FACTOR DREF IN CELL PRLIFERATION AND DEFFERENTATION

Research Project

Project/Area Number13680793
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionJAPAN WOMEN'S UNIVERSITY

Principal Investigator

MATSUKAGE Akio  Japan Women's University, Faculty of Science, Professor, 理学部, 教授 (90019571)

Project Period (FY) 2001 – 2002
Project Status Completed(Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost : ¥3,600,000)
Fiscal Year 2002 : ¥1,400,000 (Direct Cost : ¥1,400,000)
Fiscal Year 2001 : ¥2,200,000 (Direct Cost : ¥2,200,000)
KeywordsCell proliferation / transcription regulation factor / Drosophila / transcription of proliferation-related genes / chromatin structure / genome screening / 転写制御因子 / 転写プロモーター / 遺伝子導入バエ / DNAチップ / 分化 / クロマチン修飾因子 / ショウジョウバエゲノム
Research Abstract

We isolated the gene and cDNA encoding DREF (DNA replication-related element-binding factor), a Drosophila transcription factor required for the expression of many DNA replication- and proliferation-related genes. Using trangenic flies eexpressing this factor in tissue-specific manners, we confirmed that it is essential for normal cell proliferation and differentiation. We have identified the human homologue (hDREF) of DREF, whose expression is well correlated with cell proliferation. Thus, hDREF is suggested to be involved in transcription of mammalian proliferation-related genes. We have prepared specific antibodies to detect hDREF and confirmed its nuclear localization. We also identified several proteins which interact with DREF by genetic and biochemical methods. DREF interact with some proteins involved in construction and remoceling of chromatin structures such as polycomb group protein and Mi 2 DNA helicase, which suggests that DREF may activate gene expression by modfying chromatin structures. We also searched the Drosophila genome date to find out how many genes are possibly regulated by DREF, and about 150 genes are so far found. Interestingly, many of them are involved various processes of cell proliferation. Therefore, DREF may be one of the master keys for cell proliferation.

Report

(3results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report

Research Products

(31results)

All Other

All Publications

  • [Publications] Kwon, E.-J., Oh, E.-J., Kim, Y.-S., Hirose, F., Ohno, K., Nishida, Y., Matsukage, A., Yamaguchi, M., Yoo.M.-A.: "Transcriptional regulation of the Drosophila raf proto-oncogene by the transcription"Nucleic Acids Res.. 29. 1808-1814 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaguchi, M., Yoshida H., Hirose, H., Inoue, Y.H., Hayashi, Y., Yamagishi, M., Nishi, Y., Tamai, K., Sakaguchi, K., Matsukage, A.: "Ectopic expression of BEAF32A in the Drosophila eye imaginal disc inhibits differentiation of photoreceptor cells and induces apoptosis"Chromosoma. 110. 313-321 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshida, H., Inoue, Y.H., Hirose, F., Sakaguchi, K., Matsukage, A., Yamaguchi, M.: "Overexpression of DREF in the Drosophila wing imaginal disc induces apoptosis and a notching wing phenotype"Genes to Cells. 6. 1-11 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirose, F., Ohshima, N., Shiraki, M., Inoue, Y.H., Taguchi, O., Nishi, Y., Matsukage, A., Yamaguchi, M.: "Ectopic expression of DREF induces DNA synthesis, apoptosis and unusual morphogenesisi in the Drosophila eye imaginal disc : Possible interaction with Polycomb and trithorax group proteins"Mol.Cel.Biol.. 21. 7231-7242 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizushina, Y., Sugiyama, Y., Yoshida, H., Hanashima, S., Yamazaki, T., Kamisuki, S., Ohta, K., Takemura, M., Yamaguchi, T., Matsukage, A., Yoshida, S., Saneyoshi, M., Sugawara, F., Sakaguchi, K.: "Galacosyldiacylglycerol, a mammalian DNA polymerase a-specific inhibitor from a sea algae, Petalonia bingbamiae"Biol.Pharm.Bull.. 24. 982-987 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizushina, Y., Kamisuki, S., Kasai, N., Shimazaki, N., Takemura, M., Asahara, H., Linn, S., Yoshida, S., Matsukage, A., Koiwai, O., Sugawara, F., Yoshida, H., Sakaguchi, K.: "A plant phytotoxin, salanapyrone A, is an inhibitor of DNA polymerase and λ"J.Biol.Chem.. 277. 630-638 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 松影昭夫: "DNAポリメラーゼβ"生化学. 74. 203-207 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 松影昭夫: "転写制御因子DREFは細胞増殖のマスターキーか?"日本女子大学理学部紀要. 10. 73-79 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Maruyama, M, Inoue, M, Koike, A., Shuto, T., Ikeda, A., Yoshida, H, Yamaguchi, M., Hirose, F., Matsukage, A.: "DREF, a transcriptional regulatory factor related to cell proliferation"Natural Science Report of the Ochanomizu University. 53. 55-62 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 松影昭夫, 平井久雄(共著編): "ゲノムの複製と分配"シュプリンガー・フェアラーク東京. 234 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kwon, E.-J., Oh, E.-J., Kim, Y.-S., Hirose, F., Ohno, K., Nishida, Y., Matsukage, A., Yamaguchi, M. and Yoo. M.-A.: "Transcriptional regulation of the Drosophila raf proto-oncogene by the transcription factor dE2F."Nucleic Acids Res.. 29. 1808-1814 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaguchi, M., Yoshida, H., Hirose, F., Inoue, Y. H,. Hayashi, Y., Yamagishi, M., Nishi, Y., Tamai, K., Sakaguchi, K. and Matsukage, A.: "Ectopic expression of BEAF32A in the Drosophila eye imaginal disc inhibits differentiation of photoreceptor cells and induces apoptosis."Chiomosoma. 110. 313-321 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshida, H., Inoue, Y.H., Hirose, F., Sakaguchi, K., Matsukage, A. and Yamaguchi, M.: "Overexpression of DREF in the Drosophila wing imaginal disc induces apoptosis and a notching wing phenotype."Genes to Cells. 6. 1-11 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirose, F., Ohshima, N., Shiraki, M., Inoue, Y. H., Taguchi, O., Nishi, Y., Matsukage, A. and Yamaguchi, M.: "Ectopic expression of DREF induces DNA synthesis, apoptosis and unusual morphogenesisi in the Drosophila eye imaginal disc: Possible interaction with Polycomb and trithorax group proteins."Mol. Cel. Biol.. 21. 7231-7242 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizushina, Y., Sugiyama, Y., Yoshida, H., Hanashima, S., Yamazaki, T., Kamisuki, S., Ohta, K., Taskemura, M., Yamaguchi, T., Matsukage, A., Yoshida, S., Saneyoshi, M., Sugawara, F. and Sakaguchi, K.: "Galacosyldiacylglycerol, a mammalian DNA polymerase a-specific inhibitor from a sea algae, Petalonia bingbamiae."Biol. Pharma. Bull.. 24. 982-987 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizushina, Y., Kamisuki, S., Kasai, N., Shimazaki N., Takemura, M., Asahara, H., Linn, S., Yoshida, S., Matsukage, A., Koiwai, O., Sugawara, F., Yoshida, H. and Sakaguchi, K.: "A plant phytotoxin, salanapyrone A, is an inhibitor of DNA polymerase β and λ."J. Biol. Chem.. 277. 630-638 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsukage, A.: "DNA polymerase β"Biochemistry. 74. 203-207 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsukage, A.: "Is the Transcription Regulatory Factor DREF a Master Key for Cell Proliferation?"J Japan Women's Univ., Fac. of Science. 10. 73-79 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Maruyama, M., Inoue, M., Koike, A., Shuto, T., Ikteda, A., Yoshida, H., Yamaguchi, M., Hirose F. and Matsukage, A.: "DREF, a transcriptional regulatory factor related to cell proliferation."Natural Science Report of the Ochanomizu University. 53. 55-62 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsukage, A. and Masai, Y. eds.: "Genome, Replication and Differentiation."Springer-Verlag Tokyo. 234 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Mizushina他: "A plant phytotoxin, salanapyrone A, is an inhibitor of DNA polymerase β and λ"J.Biol.Chem.. 277. 630-638 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Higashiyama他: "Identification of ter94, Drosophila VCP, as a Modulator of polyglutamine-indeeced neurodegeneration"Cell Death and Differentiation. 9. 364-373 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Maruyama他: "DRDF, a transcriptional regulatory factor related to cell proliferation"Natural Science Report of the Ochanomizu University. 53. 55-62 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松影昭夫: "転写因子DREFは細胞増殖のマスターキーか?"日本女子大学紀要理学部. 10. 73-79 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松影昭夫他: "ゲノムの複制と分配"シュプリンガー・フェアラーク東京. 234 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kwon, E.-J.: "Transcriptional regulation of the Drosophila rat proto-oncogene by the transcription, factor dEZF"Nucleic Acids Res.. 29. 1808-1814 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaguchi, M.: "Ectopic expression of BEAF32A in the Drosophila eye imaginal disc inhibits differentiation of photoreceptor cells and induces apoptosis"Chromosoma. 110. 313-321 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshida, H.: "Overexpression of DREF in the Drosophila wing imaginal disc induces apoptosis and a notching wing phenotype"Genes to Cells. 6. 1-11 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirose, F.: "Ectopic expression of DREF induces DNA synthesis, apoptosis and unusual morphogenesis in the Drosophila eye imaginal disc"Mol. Cel. Biol.. 21. 7231-7242 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mizushina, Y.: "A plant phytotoxin, salanapyrone A, is an inhibitor of DNA polymerase β and λ"J. Biol. Chem.. 277. 630-638 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松影昭夫: "DNAポリメラーゼβ"生化学. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published : 2001-04-01   Modified : 2016-04-21  

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